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RIPA and UltraRIPA Lysis Buffers - Ready-to-Use 1x Protein Extraction Buffers

Product#: Buffers-RIPA-UltraRIPA-LysisBuffers
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Lysis & Extraction Buffers

RIPA and UltraRIPA Lysis Buffers Ready-to-Use 1x Protein Extraction Buffers

Two chemistries. One of them reaches the pellet.

Ready-to-use 1x lysis reagents from Funakoshi, distributed by Diagnocine. Which one you want comes down to a single question: does your target stay behind in the RIPA-insoluble fraction?

FOR RESEARCH USE ONLY — NOT FOR USE IN HUMANS
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Two chemistries, three sizes

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RIPA Buffer
One formulation, two sizes. Same Tris / NaCl / detergent system in both bottles.
100 mL FNK-F015A-100
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250 mL FNK-F015A-250
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UltraRIPA™ Buffer
Non-denaturing chemistry that reaches the RIPA-insoluble fraction.
100 mL FNK-F015B
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Not sure which chemistry fits your target? The comparison below takes about a minute.

Quotes, purchase orders, and institutional procurement portals: contact support@diagnocine.com or call 551-245-1033. Certificates of Analysis are available on request with your lot number.
What each buffer recovers

Solubilization reach, side by side

Standard RIPA clears the cytosol and most membrane-associated protein. Detergent-resistant membrane (DRM) and lipid raft material stays in the pellet. You can force it out with 2% SDS — but the protein comes out denatured.

 
Cytosolic
Membrane-associated
DRM / lipid raft
RIPA Buffer FNK-F015A
Native state kept
UltraRIPA™ Buffer FNK-F015B
Native state kept   >70% of pellet
2% SDS buffer reference only
Denaturing — no activity readout

Reach is shown by fraction, not by yield. The UltraRIPA™ figure is protein recovered from a pellet that standard RIPA buffer had already left behind.

Choose

Pick by what you need to recover

If your target is soluble or membrane-associated

RIPA Buffer

The everyday choice for whole-cell and tissue lysis feeding Western blot, immunoprecipitation, and ELISA sample prep. Fully disclosed formulation, no protease inhibitors added, so you pick the cocktail.

100 mL 250 mL
If your target sits in the RIPA-insoluble pellet

UltraRIPA™ Buffer

Recovers more than 70% of protein from that pellet without denaturing it — so lipid raft and other DRM-associated proteins stay usable for immunoprecipitation and enzyme activity assays.

100 mL
Running both is common: lyse with RIPA Buffer, spin, then re-extract the pellet with UltraRIPA™ Buffer to get the fraction the first pass left behind.
Specifications

Chemistry and handling, compared

Both sizes of RIPA Buffer share one formulation, so the two chemistries below are the real decision.

Parameter RIPA Buffer FNK-F015A UltraRIPA™ Buffer FNK-F015B
Composition 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1% NP-40 alternative, 0.1% SDS, 0.5% sodium deoxycholate Proprietary non-denaturing detergent system; itemized breakdown not published
pH 8.0 Not published
Solubilization conditions Limits protein denaturation Non-denaturing
DRM / lipid raft recovery Not solubilized >70% of protein from the RIPA-insoluble fraction
Enzyme activity retained (LDH) Equivalent to Triton X-100 Equivalent to Triton X-100 and RIPA buffer
Protease inhibitors Not included — add your own Not included — add your own
Format 1x, ready to use 1x, ready to use
Sizes 100 mL, 250 mL 100 mL
Storage / shelf life 4°C / 1 year from receipt 4°C / 1 year from receipt
Typical use Western blot, IP and co-IP, ELISA sample prep, total protein extraction DRM and lipid raft recovery, IP, enzyme activity assays
UNSPSC 12161703 12161703
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Before you lyse

Working notes

Add inhibitors yourself

Neither buffer ships with protease inhibitors. Add your cocktail — and phosphatase inhibitors if you are reading phosphorylation state — immediately before use.

Check assay compatibility

RIPA Buffer contains SDS and deoxycholate. Confirm detergent tolerance for your protein quantitation method and for any antibody-based step that needs native conformation.

No dilution step

Both products arrive as 1x working solutions and are used as received. There is nothing to reconstitute, so there is no preparation step to vary between users.

Store at 4°C on arrival

Both buffers are stored at 4°C and carry a 1 year shelf life from the date of receipt. Neither is intended for clinical, diagnostic, or therapeutic use.

FAQ

Frequently asked questions

Start with RIPA Buffer for routine total protein extraction. Move to UltraRIPA™ Buffer when your target is membrane-raft associated, when it keeps showing up in the insoluble pellet, or when the readout downstream depends on the protein staying functional.
Yes. A sequential extraction is the standard way to separate the two fractions: lyse in RIPA Buffer, clear by centrifugation, then re-extract the remaining pellet with UltraRIPA™ Buffer to recover the DRM / lipid raft material.
No. Both are supplied without protease inhibitors so you can match the cocktail to your target proteins. Add inhibitors immediately before use.
No. UltraRIPA™ Buffer is a proprietary detergent formulation and its pH, molarity, and component breakdown are not published. RIPA Buffer, by contrast, is fully specified: 50 mM Tris-HCl at pH 8.0, 150 mM NaCl, 1% NP-40 alternative, 0.1% SDS, and 0.5% sodium deoxycholate.
Volume only. FNK-F015A-100 is 100 mL and FNK-F015A-250 is 250 mL; the formulation, storage, and shelf life are identical.
No. All three products are labeled For Research Use Only and are not intended for clinical, diagnostic, or therapeutic use in humans.
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