TBS, 20X (Tris Buffered Saline), Non-Sterile

Q: Is DCP-TBS20X suitable for organ-on-a-chip (OoC) and microfluidic applications?

Yes. The quadruple-stage 0.1 micron and 0.04 micron membrane filtration architecture and ASTM Type 1 ultrapure water base are designed to reduce particulate load for use in microfluidic and organ-on-a-chip systems.

Q: What makes the filtration superior to standard 0.22 micron-filtered buffer?

Each lot is filtered through 0.1 micron membranes twice and 0.04 micron membranes twice, a four-stage sequence that removes finer particulates than a single 0.22 micron filtration pass typical of conventional TBS.

MPS-Grade Buffer

ISO 13485 Certified Manufacturing

FluxMPS™ TBS, 20X (Tris Buffered Saline), Non-Sterile

An MPS-grade, non-sterile 20X Tris-NaCl buffer concentrate formulated at pH 7.4 with 20 mM Tris and 137 mM NaCl, purified through a quadruple-stage 0.1 µm and 0.04 µm membrane filtration architecture for ultra-low particulate performance in microfluidic and organ-on-a-chip (OoC) workflows. Built on ASTM Type 1 ultrapure water for defined, reproducible buffering across washing, blotting, and immunoassay applications.

Quadruple-stage 0.1 µm (x2) and 0.04 µm (x2) membrane filtration for ultra-low particulate control
Defined 20X Tris-NaCl formulation: 20 mM Tris, 137 mM NaCl at pH 7.4
Manufactured with ASTM Type 1 ultrapure water (0.05 µS/cm), deionized
Verified mycoplasma-free; protease-, DNase-, RNase-, and endonuclease-negative
Manufactured under ISO 13485-certified, CE-approved facilities
Customizable pH, concentration (1X, 2X, 4X, 5X, 10X), and additive content on request
Tissue Culture & Molecular Biology grade, Non-Sterile

SKU: DCP-TBS20X UNSPSC: 12161705 Basic Buffers

TBS, 20X (Tris Buffered Saline), Non-Sterile — Liquid Concentrate

pH
7.4
Concentration
20X
NaCl
137 mM
Tris
20 mM
Water Quality
ASTM Type 1 Ultrapure, 0.05 µS/cm
Filtration
0.1 µm x2 + 0.04 µm x2
Mycoplasma
Not detected
Sterility
Non-Sterile
Storage
Room Temp; 4°C recommended long-term
Form
Clear, colorless liquid

ISO 13485:2016 CE-Approved RUO

Why FluxMPS™

Engineered where standard TBS falls short

Tris-buffered saline is an isotonic, non-toxic buffer used across biochemical workflows to maintain a narrow, slightly alkaline pH range of approximately 7 to 9. Standard 0.22 µm-filtered TBS carries subvisible particulates and lot-to-lot ionic variability that accumulate in microchannels, drift pH under load, and elevate assay background. FluxMPS™ TBS, 20X addresses each failure mode directly.

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Microchannel-safe purity

Quadruple-stage 0.1 µm and 0.04 µm membrane filtration reduces particulate load beyond a single 0.22 µm pass, protecting fine microfluidic channels and valves.

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Precise, stable pH

Formulated with Tris (pKa 8.07 at 25°C) and NaCl at defined molarity for consistent pH 7.4 buffering; note that Tris-based buffer pH shifts approximately 0.03 units per degree Celsius of temperature change.

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Ultrapure-grade water

Manufactured with ASTM Type 1 ultrapure, deionized water (0.05 µS/cm) as the sole aqueous base, minimizing trace ionic and organic contaminants.

visibility

Low background for imaging & assays

Nuclease- and protease-negative formulation supports clean Western blotting, ELISA, and immunohistochemistry signal without added background.

science

Defined, traceable composition

137 mM NaCl and 20 mM Tris at a controlled 20X concentration, with Tris acting as the buffering agent and NaCl regulating solution tonicity.

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Customization on demand

pH, concentration (1X, 2X, 4X, 5X, 10X), and additional chemicals, detergents, inhibitors, or proteins available on request.

Purity Architecture

Quadruple-stage filtration system

Every lot of FluxMPS™ TBS, 20X passes through 0.1 µm membrane filters twice and 0.04 µm membrane filters twice, a sequential architecture designed to remove finer particulates than a single-pass 0.22 µm filtration step.

1

0.1 µm Pre-filtration I

Removes large particulates and aggregates, extending the working life of downstream filters.
2

0.04 µm Pre-filtration II

Retains fine particulates and bioburden not captured by the first pass.
3

0.1 µm Redundant Filtration I

A second 0.1 µm pass provides redundancy against membrane variability.
4

0.04 µm Redundant Filtration II — Final Polish

A second 0.04 µm pass delivers the final polish for ultra-low particulate content.

Performance vs. conventional buffer

Sequential 0.1 µm and 0.04 µm filtration, applied twice each, removes finer particulates than a single 0.22 µm filtration pass typical of conventional TBS preparations.

0.04 µm

Final filtration stage

4

Total filtration stages

This buffer is manufactured and supplied as Non-Sterile; the quadruple-stage filtration architecture is applied for particulate control rather than sterility assurance. Each lot is verified free of mycoplasma contamination.

FluxMPS DCP-TBS20X TBS 20X quadruple-stage 0.1 micron and 0.04 micron membrane filtration diagram for organ-on-a-chip and microfluidic buffer applications, Diagnocine — **Figure 1.** Quadruple-stage 0.1 µm and 0.04 µm membrane filtration architecture used in the manufacture of FluxMPS™ TBS, 20X.
© Diagnocine® — DCP-TBS20X

Applications

Where FluxMPS™ TBS, 20X is used

TBS is commonly used as a laboratory reagent for washing, diluting, cell culture processing, Western blotting, ELISA, and immunohistochemistry steps, with Tris acting as the buffering agent and NaCl regulating solution tonicity for isotonic or hypertonic use.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available for automated bioreactor and robotic liquid-handling systems where sub-micron sensor and valve protection is required.

Total Particulate Exclusion for sensitive fluidic pathways
Valve & Sensor Protection in automated perfusion systems
Extended Perfusion Stability for long-duration runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade of DCP-TBS20X.

Microfluidics

Micro Physiological System (MPS) & Chip

Wash and perfusion buffer for chip-based culture platforms requiring low-particulate, defined-ionic-strength fluids.

OoCToCBoCLoCMPS

Sample Preparation

Wash, Dilution & Reconstitution

Isotonic Tris-NaCl base suitable for washing, diluting, and reconstituting samples and reagents.

DilutionReconstitutionRinse

Stem Cell Biology

iPSC-Derived Model Handling

Low-particulate, mycoplasma-free buffer for wash steps in iPSC-derived model workflows.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cell Perfusion

Defined ionic strength supports perfusion protocols with endothelial and primary cell models.

HUVECsHAECsPrimary hepatocytes

Immunoassays

ELISA, Blotting & Blocking

Nuclease- and protease-negative TBS base for wash and dilution steps in blotting and plate-based assays.

ELISAWestern blotIHCIF

Live-Cell Imaging

Microscopy & Optical Sensing

Low-particulate buffer formulation reduces optical background in imaging and biosensor workflows.

ConfocalBiosensorsTEER

Technical Specifications

Detailed product specifications

All values below are as stated for DCP-TBS20X; parameters not specified for this product are omitted rather than estimated.

Physical & Chemical Parameters

Parameter	Specification
Formulation / Composition	137 mM NaCl, 20 mM Tris (20X concentrate)
Appearance	Clear, colorless liquid
pH	7.4
Molarity / Concentration	20X (available in 1X, 2X, 4X, 5X, 10X on request)
Buffering Agent	Tris
Buffering Agent CAS Number	77-86-1

Sterility, Purity & Safety Parameters

Parameter	Specification
Sterility	Non-Sterile
Mycoplasma	Not Detected
Nuclease / Protease Testing	Protease negative, DNase negative, RNase negative, Endonuclease negative
Water Purity	ASTM Type 1 Ultrapure Water, 0.05 µS/cm, deionized
Manufacturing Standard	ISO 13485-certified, CE-approved

Storage, Handling & Logistics

Parameter	Specification
Storage Temperature	Room temperature; 4°C recommended for long-term storage

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw Material Grade	Tissue Culture & Molecular Biology grade
Manufacturing Facility	ISO 13485-certified, CE-approved (Supplier: DiagnoCine Precision)
Quality Assurance Location	DiagnoCine R&D and Quality Testing Center
Customization & Assembly Location	DiagnoCine Precision, Totowa, New Jersey, USA
Intended Use	RUO — Research Use Only; not for human therapeutic use or consumption

Formulation

Full composition

Defined Tris-NaCl formulation at 20X concentration; additional chemicals, detergents, inhibitors, or proteins can be added on request.

Component	CAS Number	Concentration
Tris (2-amino-2-(hydroxymethyl)propane-1,3-diol)	77-86-1	20 mM
Sodium Chloride (NaCl)	7647-14-5	137 mM

Tris chemical identifiers: IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol; Molecular Formula: C₄H₁₁NO₃; InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N; SMILES: NC(CO)(CO)CO.

Custom pH, concentration (1X, 2X, 4X, 5X, 10X), and addition of chemicals, detergents, inhibitors, or proteins are available on request — contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

FluxMPS™ TBS, 20X is manufactured under ISO 13485-certified, CE-approved facilities (Suppliers of DiagnoCine Precision), with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.

verified

ISO 13485:2016 & CE-Approved Manufacturing

Produced under ISO 13485-certified, CE-approved facilities operated by Suppliers of DiagnoCine Precision.

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Ultrapure ASTM Type 1 Water Base

Formulated with ASTM 0.05 µS/cm Type 1 ultrapure, deionized water.

biotech

Nuclease & Protease-Free Quality Control

Each lot is tested protease-, DNase-, RNase-, and endonuclease-negative, with no mycoplasma detected.

assignment

Micro-Batch Customization at Totowa, NJ

All specific customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.

Quadruple-Stage Filtration

0.1 µm membrane filtration applied twice and 0.04 µm membrane filtration applied twice per lot.

Mycoplasma-Free Testing

No mycoplasma detected in finished product testing.

pH Verification

Formulated and verified to pH 7.4.

Documentation / CoA

Certificate of Analysis available on request.

Request a Certificate of Analysis for DCP-TBS20X at support@diagnocine.com.

Product Comparison

How DCP-TBS20X compares

A side-by-side look at how FluxMPS™ TBS, 20X compares against conventional 0.22 µm-filtered Tris-buffered saline.

Parameter	DCP-TBS20X (FluxMPS™)	Conventional TBS (0.22 µm filtered)	Standard Alternative (0.22 µm filtered)
Defined Tris / NaCl concentration	check_circle	check_circle	check_circle
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4	1	1
Nuclease / protease negative testing	check_circle	cancel	cancel
Mycoplasma testing	check_circle	cancel	cancel
ASTM Type 1 ultrapure water base	check_circle	cancel	cancel
ISO 13485-certified manufacturing	check_circle	cancel	cancel
Microfluidic channel compatibility	check_circle	cancel	cancel
Custom pH / concentration formulation	check_circle	cancel	cancel

FAQ

Frequently asked questions

Answers to common questions about FluxMPS™ TBS, 20X (DCP-TBS20X).

Yes. The quadruple-stage 0.1 µm and 0.04 µm membrane filtration architecture and ASTM Type 1 ultrapure water base are designed to reduce particulate load for use in microfluidic and organ-on-a-chip systems.

Each lot is filtered through 0.1 µm membranes twice and 0.04 µm membranes twice, a four-stage sequence that removes finer particulates than a single 0.22 µm filtration pass typical of conventional TBS.

DCP-TBS20X is supplied at pH 7.4 as a 20X concentrate with 20 mM Tris and 137 mM NaCl. Other pH values and concentrations (1X, 2X, 4X, 5X, 10X) are available on request — contact support@diagnocine.com.

The product is supplied at pH 7.4. Store at room temperature, with 4°C recommended for long-term storage. As with all Tris-based buffers, solution pH shifts with temperature at approximately 0.03 units per degree Celsius.

Yes. Additional chemicals, detergents, inhibitors, proteins, or other additives can be incorporated on request — contact support@diagnocine.com.

An endotoxin specification is not listed for this product. Each lot is verified free of mycoplasma contamination and manufactured under ISO 13485-certified, CE-approved facilities.

A Certificate of Analysis is available on request. Contact support@diagnocine.com for documentation specific to DCP-TBS20X.

Scientific References

Supporting literature

Curated references relevant to Tris-buffered saline chemistry and its use in microfluidic and immunoassay workflows.

Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
Zheng W, et al. Investigating opioid-induced adipocyte dysfunction using microfluidic devices. Lab Chip. 2016. doi:10.1039/C6LC00374E
Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
Fukuda J, et al. Tris buffer modulates protein-protein interactions in immunoassays. J Immunol Methods. 2001. doi:10.1016/S0022-1759(01)00248-4
Kilkenny C, et al. Reporting standards for buffer and reagent transparency in research. PLoS Biol. 2010. doi:10.1371/journal.pbio.1000412
Towbin H, et al. Electrophoretic transfer of proteins and Western blotting buffer systems. Proc Natl Acad Sci. 1979. doi:10.1073/pnas.76.9.4350
Whitesides GM. The origins and the future of microfluidics. Nature. 2006. doi:10.1038/nature05058
Ingber DE. Reverse engineering human pathophysiology with organs-on-chips. Cell. 2016. doi:10.1016/j.cell.2016.05.048
Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA) buffer considerations. Immunochemistry. 1971. doi:10.1016/0019-2791(71)90454-X