Tris-HCl Buffer [1X]
Cat No.: DCP-THB1X
Size: 100 mL, 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
Description:
Tris-HCl buffer is widely used in molecular biology techniques like gel electrophoresis, nucleic acid work, protein extraction and purification. It is used for preparing SDS-PAGE gel solutions, electrophoresis running buffers, and cell culture media. It is a mild buffer that does not significantly interfere with or inhibit most biochemical processes and protein-protein interactions. Its buffering capacity prevents abrupt changes in pH during purification steps, which could denature or degrade proteins.
Advantages:
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Tris-HCl provides good buffering capacity in the pH range of around 7.0 to 9.0, which is compatible with many biological molecules, enzymes, and cellular processes.
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Tris-HCl does not significantly interfere with or inhibit most biochemical reactions or enzymes or interact with common ions like calcium and magnesium.
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Tris-HCl buffers are widely used in various techniques, such as gel electrophoresis, nucleic acid work, protein extraction/purification, cell culture media, and more.
Application:
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It can be used to prepare cell lysis buffers for cell culture.
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It is used in various protein purification techniques, and purified proteins are stored in it.
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It is used in electrophoresis buffers.
Composition:
- 1M Tris base
- HCl to adjust the pH
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8 ± 0.04
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Sterility: Filtered 0.1 micron Twice + 0.04 micron twice in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration two times. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
