Diagnocoine Protocols Resources

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FLOW CYTOMETRY (source: UCSD web pages)

Cell Sorting Checklist

Fluorescent Detection of Non-Viable Cells in Fixed Cell Preparations

DNA Content of Adherent Cells

Basic Antibody Staining

Detection of Apoptosis by Propidium Iodide Only

Cell Cycle Analysis by DNA Content (Propidium Iodide)

DNAse Treatment for Clumping Cells

Ethidium Monoazide (EMA) Staining for Detecting Viable Cells

Measurement of GFP and DNA Content in Fixed Cells

Isolated Nuclei DNA Staining

Nuclear Antigens and DNA

Surface Antigens and DNA: Hypotonic Propidium Iodide Staining

Antibody Titration

Yeast Cell Cycle with SYTOX Green

Yeast Cell Cycle with SYBR Green I

20X Phosphate Buffered Saline (PBS)

Erythrocyte Lysis

Dextran Sedimentation of Erythrocytes

Paraformaldehyde Fixative


MICROBIOLOGY (source: ASM Science web pages)

ACID-FAST STAIN

ANIMAL CELLS IN CULTURE

BACTERIAL AGGLUTINATION

BACTERIAL FLAGELLA STAIN

BACTERIOLOGICAL EXAMINATION OF WATERS: MEMBRANE FILTRATION

BLOOD AGAR PLATES AND HEMOLYSIS

CAMP TEST

CAPSULE STAIN

CARBOHYDRATE FERMENTATION

CATALASE TEST

CITRATE TEST

COAGULASE TEST

COLONY MORPHOLOGY

CYTOPATHIC EFFECTS OF VIRUSES

DECARBOXYLASE BROTH

ELISA (Microbiology)

ENDOSPORE STAIN

EOSIN-METHYLENE BLUE AGAR PLATES

GELATIN HYDROLYSIS TEST

GRAM STAIN

HEKTOEN ENTERIC AGAR

INDOLE TEST

KIRBY-BAUER DISK DIFFUSION SUSCEPTIBILITY TEST

LURIA BROTH (LB) AND LURIA AGAR (LA) MEDIA

MACCONKEY AGAR PLATES

MANNITOL SALT AGAR PLATES

METHYL RED AND VOGES-PROSKAUER TEST

MOTILITY TEST MEDIUM

NITRATE AND NITRITE REDUCTION TEST

OXIDASE TEST

OXIDATIVE-FERMENTATIVE TEST

PHENYLETHYL ALCOHOL AGAR

PLAQUE ASSAY

POLYMERASE CHAIN REACTION (microbiology)

PREPARING SPREAD PLATES

SABOURAUD AGAR FOR FUNGAL GROWTH

SERIAL DILUTION

STARCH AGAR

THE STREAK PLATE

TRANSFORMATION OF ESCHERICHIA COLI MADE COMPETENT BY CALCIUM CHLORIDE

TRIPLE SUGAR IRON AGAR

UREASE TEST

USE OF EC-MUG MEDIA TO CONFIRM ESCHERICHIA COLI CONTAMINATION IN WATER SAMPLES


ANIMAL WORK (source: Iruela-Arispe Lab of UCLA web pages)

Xenograft Tumor Assay Protocol

Tamoxifen Protocol

X-Gal Staining for Expression of LacZ in Cultured Cells

WHOLE MOUNT IN SITU HYBRIDIZATION PROCEDURE FOR CHICK (or MOUSE) EMBRYOS

Protocol for evaluating vascular permeability in wildtype and PRTg mice

Prot_Animl_superovulation

OVARIAN TRANSPLANT

Mouse Bone Marrow Transplantation

Modified Matrigel Assay

STAINING PROTOCOL FOR FACS

Protocol for isolation of EC from adult liver/heart (AL)

Cutaneous Wound Healing Assay (from Monika Krampert)


BIOCHEMISTRY (source: Iruela-Arispe Lab of UCLA web pages)

Protocol for Zymogram

VEGF Western on Tricine Gel

Tricine Gel after VEGF Digestion

Silver staining

Stripping Membrane with Luisa’s Protocol

Membrane Protein Labeling

Protocol for LacZ Staining

Immunoprecipitation

Biotinylated VEGF Digestion


GENOTYPING (source: Iruela-Arispe Lab of UCLA web pages)

VE-Cadherin Cre PCR Protocol

Sex Determination Protocol

ROSA PCR

PCR genotyping ADAMTS1 KO mice

PCR Protocol for PR-CRE Recombinase Genotyping

NOTCH LOX PCR PROTOCOL

Genotyping of mice by lacZ staining

ILK PCR

DNA Genotyping Protocol

CRE PCR

CIVE PCR Protocol

BIF PCR

PCR genotyping for ADAMTS1 KO mice


IMMUNOHISTOCHEMISTRY (source: Iruela-Arispe Lab of UCLA web pages)

Whole Mount Retina IHC

VE-Cadherin Protocol

Immunocytochemistry for Myosin Light Chain For Cells in Suspension

QH-1 Antibody Staining Protocol (quail only)

PECAM Protocol (cell chamber slides)

Modified LacZ staining

HAMSTER ANTI MOUSE CD31 MONOCLONAL ANTIBODY

LacZ staining Protocol

Immunohistochemistry Protocol for Whole-Mount or Vibratome-Sectioned Tissue PECAM Antibody Staining

IHC CD31 PARAFFIN SECTIONS

STAINING PROTOCOL FOR FACS

Citrate Buffer Epitope Retrieval Method


MOLECULAR BIOLOGY (source: Iruela-Arispe Lab of UCLA web pages)

Blue/White Color Screening

Annealing of Adapters

Whole mount In Situ Hybridization protocol

LIGATIONS

RNA Protection Assay (RPA)

RNA GEL

RNA EXTRACTION SOLUTIONS

RNA EXTRACTION FROM CELLS

Real Time PCR protocol

MINI-PREP DNA

Genotyping TGF ??Transgenic Mice

Nanostring Counter Sample Collection SOP


PROTEIN CHEMISTRY (source: Iruela-Arispe Lab of UCLA web pages)

Protocol for making 3D vitrogen gel

Protocol for coating 100 ng/mL VEGF bound plate

Transfection of [T47D] Cells

Soft Agar Colony Formation Assay

Isolation of Ovarian Epithelial Cells

Labeling Cells with Live Dyes

Isolation of Ovarian Epithelial Cells

Iodination and Injection of 125I-Protein

Immunoprecipitation

ISOLATION OF HUMAN DERMAL ENDOTHELIAL CELLS

Crystal Violet (CV) Staining of Cells and Clone counting

CELL SURFACE BIOTINYLATION

BAEC Isolation

Adhesion Asays

ADAMTS-1 purification


SUBCLONING GUIDE (source: Iruela-Arispe Lab of UCLA web pages)

Easy Subcloning


TISSUE CULTURE (source: Iruela-Arispe Lab of UCLA web pages)

X-Gal Staining for Expression of LacZ in Cultured Cells

Polyoma middle T PRODUCTION FROM THE PACKAGING CELL LINE GgP+E.

KDR Phosphorylation Assay

Media Recipes

MAEC ISOLATION (adapted from Core Lab Cardiology)

Lentiviral Infection of Endothelial Cells (HDMEC)

ISOLATION OF ENDOTHELIAL CELLS FROM MOUSE EMBRYOS

Protocol for isolation of Endothelial cells from embryos

Isolating endothelial cells from bovine aortas

Integrin Receptor Ligand Binding Assays

Immunocytochemistry

STAINING PROTOCOL FOR FACS

CaPO4 Transfection

35 S-Methionine Metabolic Labeling


VASCULAR IMAGING (source: Iruela-Arispe Lab of UCLA web pages)

Whole-mount Lectin Staining using Vector Elite ABC Kit

ImmunohistochemistryProtocolforWhole-Mount orVibratome-SectionedTissue PECAMAntibodyStaining

ImmunohistochemistryProtocolforParaffin-Embedded Sections PECAMAntibodyStaining

Labeling Cells with Live Dyes

Immuno-Staining For CD-31

Immunoperoxidase of Paraffin Embedded Sections

Protocol for ICC from Tom Murphy

BAD Immuno-Staining For CD-31

Immunohistochemistry Protocol for Whole-Mount or Vibratome-Sectioned Tissue Notch Antibody Staining

Vibratome Protocol


Animal Techniques

Experimental Surgery

Isolation of bone marrow

Isolation of colonic epithelium

Mouse Spleenectomy

Principles of Rodent Surgery for the New Surgeon-converted

Establishing Intracranial Brain Tumor Xenografts

Isolating Xenograft Tumor Cells for Tissue Culture or Transplantation

Xenograft Tumor Model Protocol

Biochemistry: Amino Acids & Protein

Basic Protein Chemistry Techniques

Acetylation (or Succinylation) of Amino Groups on Proteins

Amicon Stirred Ultrafiltration Cells

Protein arginine methylation

Permeabilization of gram-negative bacteria with KPi-hexane for the release of L-asparaginase

Purification of acidic phosphatase from mustard seedlings

Radioiodination


Biochemistry : Lipid

Estimation of Fat in Milk Samples

Isolation of LDL

Lipoprotein Isolation

Lipid extraction

Lipid Transmethylation

Liposome Preparation

Liposome Swelling Assay

Methylation of Fatty Acids (Kropinski Method)


Bioinformatics and Statistics

PolyPro - Polynucleotide probe design

Clone Library Dereplicator

GenBank to Fasta Converter


Cell Biology

Apoptosis A Laboratory Manual of Experimental Methods

Induced Apoptosis Using Anti-Fas mAb

JC-1 Stain of Apoptotic Cells

Staining Methods for cell death Z

Tissue Culture Methods

Cell Culture Protocols

Generating Induced Pluripotent Stem Cells in Mouse Embryonic Fibroblast Cells

Pheromone Halo Assay

Annexin V Staining Protocol for Flow Cytometry

BrdU Staining Protocol for Flow Cytometry

Cell Preparation for Flow Cytometry Protocols

Colorimetric FFPE?High pH Antigen Retrieval

Colorimetric FFPE?Low pH Antigen Retrieval

Colorimetric FFPE_Trypsin Digestion

OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry

ICC Formaldehyde Fixed Cells Direct Method

ICC Formaldehyde Fixed Cells Indirect Method

ICC Formaldehyde Fixed, Permeabilized Cells Direct

ICC Formaldehyde Fixed, Permeabilized Cells Indirect Method

ICC Methanol Fixed Cells Direct Method

ICC Methanol Fixed Cells Indirect Method

ICC Unfixed Cells Direct Method

IHC Frozen Tissue Direct Method

Immunofluorescent Staining of Intracellular Antigens on Cultured Cells

IHC FFPE Tissue Proteolytic-Induced Epitope Retrieval

Immunohistochemical Staining of Formalin-Fixed Paraffin-Embedded Tissues

Pharmacological Induction of Apoptosis with Camptothecin

Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers

Staining Cell Surface Targets for Flow Cytometry

Staining Cells with eFluor Proliferation Dyes for Flow Cytometry

Staining Intracellular Antigens for Flow Cytometry

IHC Frozen Tissue Indirect Method

IHC FFPE Tissue High pH Antigen Retrieval Direct Method

IHC FFPE Tissue Low pH Antigen Retrieval Direct Method

IHC Frozen Tissue Indirect Method Purified

IHC FFPE Tissue High pH Antigen Retrieval Indirect Method

IHC FFPE Tissue Low pH Antigen Retrieval Indirect Method

IHC FFPE Tissue Trypsin Digestion Antigen Retrieval Indirect Method

Viability Staining Protocol for Flow Cytometry

ELISPOT Ready-SET-Go! Protocols

Enzyme Linked Immunosorbent Assay (ELISA)

Immunoprecipitation Protocol

Instructions for Human Assays (384 tests)

Instructions for Human High Sensitivity Assays

Instructions for Mouse Assays (384 tests)

Instructions for Mouse High Sensitivity Assays

Instructions for Platinum Human Multiplex Assays

Instructions for Platinum Human Simplex Assays

Instructions for Rat Assays (384 tests)

Preparation of Antigen Standard

ProcartaPlex Multiplex Immunoassay (Rat isotyping assays)

ProcartaPlex Multiplex Immunoassay (Human coagulation panels)

ProcartaPlex Multiplex Immunoassay (Mouse isotyping assays)

ProcartaPlex Multiplex Immunoassay (Mouse IFN alpha-IFN beta assays)

ProcartaPlex Multiplex Immunoassay For Convenience and Mix&Match Panels (Mouse and rat assays)

ProcartaPlex Multiplex Immunoassay For Convenience and Mix&Match Panels (Human, NHP, and canine assays)

ProcartaPlex Multiplex Immunoassay For Mix&Match Panels (Porcine Assays)

Western Blotting Protocol (Immunoblotting)


Developmental Biology

Lab Protocols in Developmental Biology

Lineage Analysis Using Retrovirus Vectors

In vitro Culture of Early Chick Embryos

Shell-less cultures of chick embryos

Alcian green stain for cartilage

Shell-less Cultures of Chicken Embryos

Sea Urchin Fertilization

Sea Urchin Gamete collection


Essential Data

Molecular Weight Marker

DNA Molecular Weight Markers


General Lab Techniques

Beckman Rotor Converter Tool

Centrifugation Basis

Rotor Equilibration without Counterbalance

Protocol for Trichloroacetic Acid (TCA) Precipitation

Quantification made easy


Genetics & Genomics

In situ hybridisation to alpha satellite sequences (chromosome specific)

Micronucleus Assay in Blood Lymphocytes

Microarray Protocol

cDNA Microarray Protocol

Microarray Protocol_Genetics and Genomics

Modified Eberwine (antisense) RNA Amplification Protocol

DNA methyltransferase-based single-molecule (MAPit) assay for mapping protein-DNA interactions

Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors


Histology

Histotechniques for Pathology

Histological Fixatives

Histological Staining Techniques

Processing of Microdissected Tissue for DNA Analysis

Processing of Microdissected Tissue for RNA Analysis

Slide Preparation for Laser Capture Microdissection (LCM)

Slide Preparation for Manual Microdissection


Imaging Techniques

Blood Vessel Dihydroethidium (DHE) Quantification Using Image J

Microscopy Methods


Immunology

Antibody Storage

Antigen Design and Sera Purification Tech Sheet

Preparation Of Peptide-KLH Conjugates For Immunization

Protocol for Heat-induced Epitope Retrieval (HIER)

Testing Rabbit Bleeds By Elisa

Basic ELISA Protocol

Cellular ELISA Protocol

Competitive ELISA

ELISA (Enzyme-Linked ImmunoadSorbent Assay)

ELISA Inhibition Assay

ELISA Procedure for Measuring Serum Antibody Titer

ELISA with Platelet

Gangliosides ELISA protocol

Live Cell ELISA Protocol

Peptide Inhibition ELISA Protocol

Periodate Treatment of Fixed Cell Plates


Media & Solutions

Compound solutions

How to Make Simple Solutions and Dilutions

Salt solutions

Single component solutions

Buffers

Antibiotic Concentration in Media

Buffer Listing

LB medium

Stock Solutions And Media

Molecular Biology Solutions

Phenol Equilibration

Phosphate (Sodium) buffer Chart

Preparation of Solutions

Reto s Buffers and Media Book

RNAlater

Stock Solution

Tissue culture stock solutions and media


Microbiology Protocols

Hydrolytic Activity of Bacterial Supernatants for Fungal Suppression

Probiotic Attributes of Lactic Acid Bacteria Isolates

A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites

Creation and Use of your Infectious Vector

Generation of recombinant baculoviruses by co-transfection

Retroviral infection of primary human keratinocytes (PHKs)


Animal Modeling, C. Elegans & Other Organisms

RNA Extraction from Arabidopsis

Dauer Pheromone Preparation

EMS MutagenesisM

Freezing Worm Stocks

Gene Knockout with Conventional Mutagens

Hypochlorite treatment

Integrating extrachromosomal arrays

Making Dauers with dauer pheromone

PCR on Worms

Worm Injections

Preparation of single-stranded probes from cloned cDNA


Molecular Biology ProtocolsM

Molecular Biology

Recommended Cycle Sequencing Protocols For ABI 3100

Isolation of Plasmid DNA

How to Extract DNA from Anything Living

Lance's Subcloning procedures

cDNA Library Construction Using the Gateway Technology

Clone Genes From Phage Libraries

Cloning of small RNAs with 5 Phosphate and 3 OH ends

DNA Cloning

PJBs hints for sub-cloning

miRNA Mimics

Home-made Taq Polymerase Purification

PCR clean up using 3M sodium acetate and chilled absolute ethanol

PCR reaction components

Preventing carry-over contamination with uracil-DNA glycosylase

primer extension

Setting up the laboratory to avoid contamination

Thermal Cycling Profile for Standard PCR

dsRNA synthesis Protocol

RNA interference protocol

in vivo RNAi Protocols


Neuroscience Protocols

Neuromelanin isolation and identification

Studying the Neural Basis of Adaptive Locomotor Behavior in Insects

Dissociated Cultures of Cerebellar Neurons


Physiology Protocols

Bleeding Time Test

Blood Smear Preparation and Staining

Clot Retraction Test

CMFDA Labeling of Platelet

Coating of Platelets with Antibody in vitro

Examining a Mammalian Blood Smear

Erythrophagocytosis

Platelet Preparation

Platelet-associated Ig (PAIg) Elution

Serum Separation from Whole Blood

Thrombophagocytosis

Western Blot with Platelet Protein


Plant Biology Protocols

Plant Molecular Biology Protocols

Accumulation and Fixation of Plant Metaphase Chromosomes

Isolation of Retroelement from Plant Genomic DNA

Lyophilization of cotton leaves

Measurement of Carbon Fixation Rates in Leaf Samples

Determination of Chlorophyll Content in Chloroplasts

Isolation of Chloroplasts from Spinach Leaves

Collection and Storage of Cotton Leaf Tissue

Plant Crosses


Research Tools

BioTool


Laboratory Protocols (source: Sloan Kettering Institute web pages)

Anti-En Ab Immunofluorescent Staining of Mouse Frozen Sections

Cryoprotection-and-x-gal

Double In Situ Hybridization

Free-Floating Immunohistochemistry

Immunofluorescent Staining of Mouse Frozen Sections

Immunohistochemistry on Whole Mouse Embryos

Non-Radioactive Section In Situ Hybridization for Embryos

Tamoxifen Preparation

Western Blotting

Whole Mount RNA In Situ


Cell Assay protocols (source: ITN Protocols)

Whole Blood Basophil Activation Assay

Performing Alloreactive ELISPOT Assays

Lymphocyte Proliferation Assay Using 3H-Thymidine Incorporation

Detection of IFNg and IL-10 Secreting Cells by ELISPOT


Cell/Tissue Isolation and Prep Protocols (Source: ITN Protocols)

Recovery of Frozen Mononuclear Cells

Separation and Freezing Technique for Spleen Mononuclear Cells

Thawing Frozen Mononuclear Cells

Collection and Preservation of Kidney or Liver Transplant Biopsy

Separation and Freezing Technique for Lymph Node Mononuclear Cells

Isolation and Cryopreservation of PBMC ? CPT without Plasma Collection

Separation of Mononuclear Cells from Whole Blood Using Ficoll Gradients


Whole Blood Washing and Flow Protocols (Source: ITN Protocols)

Whole Blood Washing to Remove Components that Interfere with Ab Binding


Flow Cytometry Panels Protocols (Source: ITN Protocols)

APC 1 Flow Panel

APC 2 Flow Panel

B Cell Flow Panel

Modified X-Trial T Cell Flow Panel

T Cell Exhaustion Flow Panel

Tfh-lin 1 Flow Panel

Tfh-lin 2 Flow Panel

Treg Flow Panel

X-Trial T Cell Flow Panel


RNA and DNA Protocols (Source: ITN Protocols)

Synthesizing cDNA Using ThermoScript RT-PCR Kit in 96 Plate Format

Synthesizing cDNA Using ThermoScript RT-PCR Kit

Assessing the quality of RNA using the Agilent 2100 Bioanalyzer


RNA and DNA Protocols (Source: IOWA State University Laboratory Protocols)

Golden Rice Case Study

Bt Corn vs the European Corn Borer (ECB) Activity

Chymosin Demonstration

DNA Extraction Bacteria

DNA Extraction Kiwi

DNA Extraction Onion

DNA Extraction fruit cup

DNA Extraction Smoothie

DNA Fingerprinting

DNA Transformation Ampicilin Resistance

DNA transformation Red Colony

Recombinant DNA Dual Antibiotic-Resistance Genes

DNA TRANSFORMATION Bacteria-Green Fluorescent Protein (GFP)

Evolution of Antibiotic Resistant Bacteria

High Sucrose Soybeans Invertase Teacher

High Sucrose Soybeans Invertase Student

HSSB-Thin Layer Chromatography Teacher

HSSB-Thin Layer Chromatography Student

Marker Assisted Selection of the K-Casein Allele

Pipettor Practice submitted by Jen Koenen, Hampton-Dumont High School, Hampton, Iowa

Plant Micropropagation Using African Violet Leaves

Plasmid Isolation and Analysis

Polymerase Chain Reaction PCR_Activity

Polymerase Chain Reaction PCRSimulation>

Polymerase Chain Reaction PCR-Alu-Sequence-Analysis-Beads

QuickStix™ Strip Test-Corn Leaf Tissue

QuickStix Strip Test-Corn Seed

QuickStix Strip Test-Roundup Ready Soybeans

Soybean Flavor Demonstration

Soy Drink Protocol


Cell Culture Protocols (Source: Bissell Lab, LBL-Programs)

1-HMT-3522 medium composition

2-HMT-3522 density

3-Splitting and passing HMT-3522 Cells

5-Freezing cells

9- On Top cells culture

EHS 3D Culture

Methyl cellulose

Anchorage Independence Assay using Methylcellulose

Invasion Assay

Soft Agar Assay for Colony Formation

Protein Lysis of 3D Cell Culture in Matrigel Imbedded or On Top using PBS

Isolation of T4 cells from xenograft tumors


Immunohistochemistry/Immunofluorescence Protocols (Source: Bissell Lab, LBL-Programs)

BrdU for human cells

Propidium Iodide Staining for DNA Ploidy

Occludin staining

In Situ Hybridization Method on Mammary Sections Using


Molecular and Biochemical Assays (Source: Bissell Lab, LBL-Programs)

FACS Analysis

RNA Extraction with TRIZOL

Chromatin immunoprecipitation assay


Mouse Mammary Gland Biology Protocols (Source: Bissell Lab, LBL-Programs)

Mouse mammary gland wholemounts

Primary Mammary Epithelial Cell Isolation


PCR and Sequencing Protocols (Souce: Barrick Lab, UT Austin)

Sequencing-Genotyping Primer Design>

Escherichia coli REL606 Genome Resources

Ordering Primers

Resuspending Primers

Standard PCR Polymerase Chain Reaction

Standard PCR Dpn I digestion

Overlap PCR

Agarose Gel Electrophoresis

DNA Concentration Determination

Sanger DNA Sequencing

16S rRNA Sequencing to Identify Unknown Microbes

Megaprimer whole plasmid cloning


Genetic Modification and Cloning Protocols (Souce: Barrick Lab, UT Austin)

Site-directed mutagenesis

Transformation Electrocompetent Cell

Transformation Chemically Competent Cells

Transformation General Conjugation

Assessing Transformation Efficiency

UV mutagenesis of Bacteria

Selecting Arabinose Marker Revertants

Colony Transformation

Restriction Enzyme Cloning

Gene Gorging Marker Mutations

Gene Gorging Evolved Alleles

FLP-FRT Recombination

Genome Modification

Gene replacement using pKOV vector

Gibson Cloning Assembly

Lambda red mediated ssDNA gene modification

pSLTS genomic editing for E coli

Golden Gate Assembly

Bee Microbiome Toolkit

P1 Transduction


Mutation Rates, Growth Rates and Evolutionary Stability (Souce: Barrick Lab, UT Austin)

Fluctuation Tests

Growth Rates using R studio

Evolutionary Stability of Fluorescent Protein or Chromoprotein Expression

Phage Lysate Preparation

Measuring Phage Titers

Measuring Adsorption Rate of T7 Phage

Measuring Lysis Timing of T7 Phage

Measuring Burst Size of T7 Phage


Genome Resequencing and Mutation Validation (Souce: Barrick Lab, UT Austin)

Genomic DNA Isolation

Targeted Sequencing of a Specific Gene

Mutation Rates from Genome Resequencing

Next-generation Sequencing Illumina library preparation


Experimental Evolution Protocols (Souce: Barrick Lab, UT Austin)

Competitive Fitness Assays

Ribose Operon Deletions

Mutation Accumulation for Microsatellite Strains

Bacterial Mutation Accumulation Experiments

Using Emulsions to Select by Yield


Flow Cytometry and Cell Sorting Protocols (Souce: Barrick Lab, UT Austin)

Generic Flow Cytometry guidelines


Nucleic Acid Preparation, RNA-Seq, qPCR Protocols (Souce: Barrick Lab, UT Austin)

Ethanol Precipitation

Bacterial RNA isolation

Plant RNA isolation

RNAseq Library Preparation

RT-qPCR for differential gene expression

qPCR for plasmid copy number

Denaturing Formaldehyde Gels for Verifying RNA size

Polyacrylamide Gel Electrophoresis


Protein Expression and Purification (Souce: Barrick Lab, UT Austin)

Large-scale Protein Expression in E. Coli

Running an SDS-PAGE Gel

Purifying 6xHis-Tagged Proteins from E. Coli


in vitro Enzyme Activity Assays (Souce: Barrick Lab, UT Austin)

Measuring transcription in vitro Using Broccoli and Spinach Fluorescent RNA Aptamers

Intracellular ROS detection



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