Protocol Collections
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FLOW CYTOMETRY (source: UCSD web pages)
Cell Sorting Checklist
Fluorescent Detection of Non-Viable Cells in Fixed Cell Preparations
DNA Content of Adherent Cells
Basic Antibody Staining
Detection of Apoptosis by Propidium Iodide Only
Cell Cycle Analysis by DNA Content (Propidium Iodide)
DNAse Treatment for Clumping Cells
Ethidium Monoazide (EMA) Staining for Detecting Viable Cells
Measurement of GFP and DNA Content in Fixed Cells
Isolated Nuclei DNA Staining
Nuclear Antigens and DNA
Surface Antigens and DNA: Hypotonic Propidium Iodide Staining
Antibody Titration
Yeast Cell Cycle with SYTOX Green
Yeast Cell Cycle with SYBR Green I
20X Phosphate Buffered Saline (PBS)
Erythrocyte Lysis
Dextran Sedimentation of Erythrocytes
Paraformaldehyde Fixative
MICROBIOLOGY (source: ASM Science web pages)
ACID-FAST STAIN
ANIMAL CELLS IN CULTURE
BACTERIAL AGGLUTINATION
BACTERIAL FLAGELLA STAIN
BACTERIOLOGICAL EXAMINATION OF WATERS: MEMBRANE FILTRATION
BLOOD AGAR PLATES AND HEMOLYSIS
CAMP TEST
CAPSULE STAIN
CARBOHYDRATE FERMENTATION
CATALASE TEST
CITRATE TEST
COAGULASE TEST
COLONY MORPHOLOGY
CYTOPATHIC EFFECTS OF VIRUSES
DECARBOXYLASE BROTH
ELISA (Microbiology)
ENDOSPORE STAIN
EOSIN-METHYLENE BLUE AGAR PLATES
GELATIN HYDROLYSIS TEST
GRAM STAIN
HEKTOEN ENTERIC AGAR
INDOLE TEST
KIRBY-BAUER DISK DIFFUSION SUSCEPTIBILITY TEST
LURIA BROTH (LB) AND LURIA AGAR (LA) MEDIA
MACCONKEY AGAR PLATES
MANNITOL SALT AGAR PLATES
METHYL RED AND VOGES-PROSKAUER TEST
MOTILITY TEST MEDIUM
NITRATE AND NITRITE REDUCTION TEST
OXIDASE TEST
OXIDATIVE-FERMENTATIVE TEST
PHENYLETHYL ALCOHOL AGAR
PLAQUE ASSAY
POLYMERASE CHAIN REACTION (microbiology)
PREPARING SPREAD PLATES
SABOURAUD AGAR FOR FUNGAL GROWTH
SERIAL DILUTION
STARCH AGAR
THE STREAK PLATE
TRANSFORMATION OF ESCHERICHIA COLI MADE COMPETENT BY CALCIUM CHLORIDE
TRIPLE SUGAR IRON AGAR
UREASE TEST
USE OF EC-MUG MEDIA TO CONFIRM ESCHERICHIA COLI CONTAMINATION IN WATER SAMPLES
ANIMAL WORK (source: Iruela-Arispe Lab of UCLA web pages)
Xenograft Tumor Assay Protocol
Tamoxifen Protocol
X-Gal Staining for Expression of LacZ in Cultured Cells
WHOLE MOUNT IN SITU HYBRIDIZATION PROCEDURE FOR CHICK (or MOUSE) EMBRYOS
Protocol for evaluating vascular permeability in wildtype and PRTg mice
Prot_Animl_superovulation
OVARIAN TRANSPLANT
Mouse Bone Marrow Transplantation
Modified Matrigel Assay
STAINING PROTOCOL FOR FACS
Protocol for isolation of EC from adult liver/heart (AL)
Cutaneous Wound Healing Assay (from Monika Krampert)
BIOCHEMISTRY (source: Iruela-Arispe Lab of UCLA web pages)
Protocol for Zymogram
VEGF Western on Tricine Gel
Tricine Gel after VEGF Digestion
Silver staining
Stripping Membrane with Luisa’s Protocol
Membrane Protein Labeling
Protocol for LacZ Staining
Immunoprecipitation
Biotinylated VEGF Digestion
GENOTYPING (source: Iruela-Arispe Lab of UCLA web pages)
VE-Cadherin Cre PCR Protocol
Sex Determination Protocol
ROSA PCR
PCR genotyping ADAMTS1 KO mice
PCR Protocol for PR-CRE Recombinase Genotyping
NOTCH LOX PCR PROTOCOL
Genotyping of mice by lacZ staining
ILK PCR
DNA Genotyping Protocol
CRE PCR
CIVE PCR Protocol
BIF PCR
PCR genotyping for ADAMTS1 KO mice
IMMUNOHISTOCHEMISTRY (source: Iruela-Arispe Lab of UCLA web pages)
Whole Mount Retina IHC
VE-Cadherin Protocol
Immunocytochemistry for Myosin Light Chain For Cells in Suspension
QH-1 Antibody Staining Protocol (quail only)
PECAM Protocol (cell chamber slides)
Modified LacZ staining
HAMSTER ANTI MOUSE CD31 MONOCLONAL ANTIBODY
LacZ staining Protocol
Immunohistochemistry Protocol for Whole-Mount or Vibratome-Sectioned Tissue PECAM Antibody Staining
IHC CD31 PARAFFIN SECTIONS
STAINING PROTOCOL FOR FACS
Citrate Buffer Epitope Retrieval Method
MOLECULAR BIOLOGY (source: Iruela-Arispe Lab of UCLA web pages)
Blue/White Color Screening
Annealing of Adapters
Whole mount In Situ Hybridization protocol
LIGATIONS
RNA Protection Assay (RPA)
RNA GEL
RNA EXTRACTION SOLUTIONS
RNA EXTRACTION FROM CELLS
Real Time PCR protocol
MINI-PREP DNA
Genotyping TGF ??Transgenic Mice
Nanostring Counter Sample Collection SOP
PROTEIN CHEMISTRY (source: Iruela-Arispe Lab of UCLA web pages)
Protocol for making 3D vitrogen gel
Protocol for coating 100 ng/mL VEGF bound plate
Transfection of [T47D] Cells
Soft Agar Colony Formation Assay
Isolation of Ovarian Epithelial Cells
Labeling Cells with Live Dyes
Isolation of Ovarian Epithelial Cells
Iodination and Injection of 125I-Protein
Immunoprecipitation
ISOLATION OF HUMAN DERMAL ENDOTHELIAL CELLS
Crystal Violet (CV) Staining of Cells and Clone counting
CELL SURFACE BIOTINYLATION
BAEC Isolation
Adhesion Asays
ADAMTS-1 purification
SUBCLONING GUIDE (source: Iruela-Arispe Lab of UCLA web pages)
Easy Subcloning
TISSUE CULTURE (source: Iruela-Arispe Lab of UCLA web pages)
X-Gal Staining for Expression of LacZ in Cultured Cells
Polyoma middle T PRODUCTION FROM THE PACKAGING CELL LINE GgP+E.
KDR Phosphorylation Assay
Media Recipes
MAEC ISOLATION (adapted from Core Lab Cardiology)
Lentiviral Infection of Endothelial Cells (HDMEC)
ISOLATION OF ENDOTHELIAL CELLS FROM MOUSE EMBRYOS
Protocol for isolation of Endothelial cells from embryos
Isolating endothelial cells from bovine aortas
Integrin Receptor Ligand Binding Assays
Immunocytochemistry
STAINING PROTOCOL FOR FACS
CaPO4 Transfection
35 S-Methionine Metabolic Labeling
VASCULAR IMAGING (source: Iruela-Arispe Lab of UCLA web pages)
Whole-mount Lectin Staining using Vector Elite ABC Kit
ImmunohistochemistryProtocolforWhole-Mount orVibratome-SectionedTissue PECAMAntibodyStaining
ImmunohistochemistryProtocolforParaffin-Embedded Sections PECAMAntibodyStaining
Labeling Cells with Live Dyes
Immuno-Staining For CD-31
Immunoperoxidase of Paraffin Embedded Sections
Protocol for ICC from Tom Murphy
BAD Immuno-Staining For CD-31
Immunohistochemistry Protocol for Whole-Mount or Vibratome-Sectioned Tissue Notch Antibody Staining
Vibratome Protocol
Animal Techniques
Experimental Surgery
Isolation of bone marrow
Isolation of colonic epithelium
Mouse Spleenectomy
Principles of Rodent Surgery for the New Surgeon-converted
Establishing Intracranial Brain Tumor Xenografts
Isolating Xenograft Tumor Cells for Tissue Culture or Transplantation
Xenograft Tumor Model Protocol
Biochemistry: Amino Acids & Protein
Basic Protein Chemistry Techniques
Acetylation (or Succinylation) of Amino Groups on Proteins
Amicon Stirred Ultrafiltration Cells
Protein arginine methylation
Permeabilization of gram-negative bacteria with KPi-hexane for the release of L-asparaginase
Purification of acidic phosphatase from mustard seedlings
Radioiodination
Biochemistry : Lipid
Estimation of Fat in Milk Samples
Isolation of LDL
Lipoprotein Isolation
Lipid extraction
Lipid Transmethylation
Liposome Preparation
Liposome Swelling Assay
Methylation of Fatty Acids (Kropinski Method)
Bioinformatics and Statistics
PolyPro - Polynucleotide probe design
Clone Library Dereplicator
GenBank to Fasta Converter
Cell Biology
Apoptosis A Laboratory Manual of Experimental Methods
Induced Apoptosis Using Anti-Fas mAb
JC-1 Stain of Apoptotic Cells
Staining Methods for cell death Z
Tissue Culture Methods
Cell Culture Protocols
Generating Induced Pluripotent Stem Cells in Mouse Embryonic Fibroblast Cells
Pheromone Halo Assay
Annexin V Staining Protocol for Flow Cytometry
BrdU Staining Protocol for Flow Cytometry
Cell Preparation for Flow Cytometry Protocols
Colorimetric FFPE?High pH Antigen Retrieval
Colorimetric FFPE?Low pH Antigen Retrieval
Colorimetric FFPE_Trypsin Digestion
OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry
ICC Formaldehyde Fixed Cells Direct Method
ICC Formaldehyde Fixed Cells Indirect Method
ICC Formaldehyde Fixed, Permeabilized Cells Direct
ICC Formaldehyde Fixed, Permeabilized Cells Indirect Method
ICC Methanol Fixed Cells Direct Method
ICC Methanol Fixed Cells Indirect Method
ICC Unfixed Cells Direct Method
IHC Frozen Tissue Direct Method
Immunofluorescent Staining of Intracellular Antigens on Cultured Cells
IHC FFPE Tissue Proteolytic-Induced Epitope Retrieval
Immunohistochemical Staining of Formalin-Fixed Paraffin-Embedded Tissues
Pharmacological Induction of Apoptosis with Camptothecin
Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers
Staining Cell Surface Targets for Flow Cytometry
Staining Cells with eFluor Proliferation Dyes for Flow Cytometry
Staining Intracellular Antigens for Flow Cytometry
IHC Frozen Tissue Indirect Method
IHC FFPE Tissue High pH Antigen Retrieval Direct Method
IHC FFPE Tissue Low pH Antigen Retrieval Direct Method
IHC Frozen Tissue Indirect Method Purified
IHC FFPE Tissue High pH Antigen Retrieval Indirect Method
IHC FFPE Tissue Low pH Antigen Retrieval Indirect Method
IHC FFPE Tissue Trypsin Digestion Antigen Retrieval Indirect Method
Viability Staining Protocol for Flow Cytometry
ELISPOT Ready-SET-Go! Protocols
Enzyme Linked Immunosorbent Assay (ELISA)
Immunoprecipitation Protocol
Instructions for Human Assays (384 tests)
Instructions for Human High Sensitivity Assays
Instructions for Mouse Assays (384 tests)
Instructions for Mouse High Sensitivity Assays
Instructions for Platinum Human Multiplex Assays
Instructions for Platinum Human Simplex Assays
Instructions for Rat Assays (384 tests)
Preparation of Antigen Standard
ProcartaPlex Multiplex Immunoassay (Rat isotyping assays)
ProcartaPlex Multiplex Immunoassay (Human coagulation panels)
ProcartaPlex Multiplex Immunoassay (Mouse isotyping assays)
ProcartaPlex Multiplex Immunoassay (Mouse IFN alpha-IFN beta assays)
ProcartaPlex Multiplex Immunoassay For Convenience and Mix&Match Panels (Mouse and rat assays)
ProcartaPlex Multiplex Immunoassay For Convenience and Mix&Match Panels (Human, NHP, and canine assays)
ProcartaPlex Multiplex Immunoassay For Mix&Match Panels (Porcine Assays)
Western Blotting Protocol (Immunoblotting)
Developmental Biology
Lab Protocols in Developmental Biology
Lineage Analysis Using Retrovirus Vectors
In vitro Culture of Early Chick Embryos
Shell-less cultures of chick embryos
Alcian green stain for cartilage
Shell-less Cultures of Chicken Embryos
Sea Urchin Fertilization
Sea Urchin Gamete collection
Essential Data
Molecular Weight Marker
DNA Molecular Weight Markers
General Lab Techniques
Beckman Rotor Converter Tool
Centrifugation Basis
Rotor Equilibration without Counterbalance
Protocol for Trichloroacetic Acid (TCA) Precipitation
Quantification made easy
Genetics & Genomics
In situ hybridisation to alpha satellite sequences (chromosome specific)
Micronucleus Assay in Blood Lymphocytes
Microarray Protocol
cDNA Microarray Protocol
Microarray Protocol_Genetics and Genomics
Modified Eberwine (antisense) RNA Amplification Protocol
DNA methyltransferase-based single-molecule (MAPit) assay for mapping protein-DNA interactions
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
Histology
Histotechniques for Pathology
Histological Fixatives
Histological Staining Techniques
Processing of Microdissected Tissue for DNA Analysis
Processing of Microdissected Tissue for RNA Analysis
Slide Preparation for Laser Capture Microdissection (LCM)
Slide Preparation for Manual Microdissection
Imaging Techniques
Blood Vessel Dihydroethidium (DHE) Quantification Using Image J
Microscopy Methods
Immunology
Antibody Storage
Antigen Design and Sera Purification Tech Sheet
Preparation Of Peptide-KLH Conjugates For Immunization
Protocol for Heat-induced Epitope Retrieval (HIER)
Testing Rabbit Bleeds By Elisa
Basic ELISA Protocol
Cellular ELISA Protocol
Competitive ELISA
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
ELISA Inhibition Assay
ELISA Procedure for Measuring Serum Antibody Titer
ELISA with Platelet
Gangliosides ELISA protocol
Live Cell ELISA Protocol
Peptide Inhibition ELISA Protocol
Periodate Treatment of Fixed Cell Plates
Media & Solutions
Compound solutions
How to Make Simple Solutions and Dilutions
Salt solutions
Single component solutions
Buffers
Antibiotic Concentration in Media
Buffer Listing
LB medium
Stock Solutions And Media
Molecular Biology Solutions
Phenol Equilibration
Phosphate (Sodium) buffer Chart
Preparation of Solutions
Reto s Buffers and Media Book
RNAlater
Stock Solution
Tissue culture stock solutions and media
Microbiology Protocols
Hydrolytic Activity of Bacterial Supernatants for Fungal Suppression
Probiotic Attributes of Lactic Acid Bacteria Isolates
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
Creation and Use of your Infectious Vector
Generation of recombinant baculoviruses by co-transfection
Retroviral infection of primary human keratinocytes (PHKs)
Animal Modeling, C. Elegans & Other Organisms
RNA Extraction from Arabidopsis
Dauer Pheromone Preparation
EMS MutagenesisM
Freezing Worm Stocks
Gene Knockout with Conventional Mutagens
Hypochlorite treatment
Integrating extrachromosomal arrays
Making Dauers with dauer pheromone
PCR on Worms
Worm Injections
Preparation of single-stranded probes from cloned cDNA
Molecular Biology ProtocolsM
Molecular Biology
Recommended Cycle Sequencing Protocols For ABI 3100
Isolation of Plasmid DNA
How to Extract DNA from Anything Living
Lance's Subcloning procedures
cDNA Library Construction Using the Gateway Technology
Clone Genes From Phage Libraries
Cloning of small RNAs with 5 Phosphate and 3 OH ends
DNA Cloning
PJBs hints for sub-cloning
miRNA Mimics
Home-made Taq Polymerase Purification
PCR clean up using 3M sodium acetate and chilled absolute ethanol
PCR reaction components
Preventing carry-over contamination with uracil-DNA glycosylase
primer extension
Setting up the laboratory to avoid contamination
Thermal Cycling Profile for Standard PCR
dsRNA synthesis Protocol
RNA interference protocol
in vivo RNAi Protocols
Neuroscience Protocols
Neuromelanin isolation and identification
Studying the Neural Basis of Adaptive Locomotor Behavior in Insects
Dissociated Cultures of Cerebellar Neurons
Physiology Protocols
Bleeding Time Test
Blood Smear Preparation and Staining
Clot Retraction Test
CMFDA Labeling of Platelet
Coating of Platelets with Antibody in vitro
Examining a Mammalian Blood Smear
Erythrophagocytosis
Platelet Preparation
Platelet-associated Ig (PAIg) Elution
Serum Separation from Whole Blood
Thrombophagocytosis
Western Blot with Platelet Protein
Plant Biology Protocols
Plant Molecular Biology Protocols
Accumulation and Fixation of Plant Metaphase Chromosomes
Isolation of Retroelement from Plant Genomic DNA
Lyophilization of cotton leaves
Measurement of Carbon Fixation Rates in Leaf Samples
Determination of Chlorophyll Content in Chloroplasts
Isolation of Chloroplasts from Spinach Leaves
Collection and Storage of Cotton Leaf Tissue
Plant Crosses
Research Tools
BioTool
Laboratory Protocols (source: Sloan Kettering Institute web pages)
Anti-En Ab Immunofluorescent Staining of Mouse Frozen Sections
Cryoprotection-and-x-gal
Double In Situ Hybridization
Free-Floating Immunohistochemistry
Immunofluorescent Staining of Mouse Frozen Sections
Immunohistochemistry on Whole Mouse Embryos
Non-Radioactive Section In Situ Hybridization for Embryos
Tamoxifen Preparation
Western Blotting
Whole Mount RNA In Situ
Cell Assay protocols (source: ITN Protocols)
Whole Blood Basophil Activation Assay
Performing Alloreactive ELISPOT Assays
Lymphocyte Proliferation Assay Using 3H-Thymidine Incorporation
Detection of IFNg and IL-10 Secreting Cells by ELISPOT
Cell/Tissue Isolation and Prep Protocols (Source: ITN Protocols)
Recovery of Frozen Mononuclear Cells
Separation and Freezing Technique for Spleen Mononuclear Cells
Thawing Frozen Mononuclear Cells
Collection and Preservation of Kidney or Liver Transplant Biopsy
Separation and Freezing Technique for Lymph Node Mononuclear Cells
Isolation and Cryopreservation of PBMC ? CPT without Plasma Collection
Separation of Mononuclear Cells from Whole Blood Using Ficoll Gradients
Whole Blood Washing and Flow Protocols (Source: ITN Protocols)
Whole Blood Washing to Remove Components that Interfere with Ab Binding
Flow Cytometry Panels Protocols (Source: ITN Protocols)
APC 1 Flow Panel
APC 2 Flow Panel
B Cell Flow Panel
Modified X-Trial T Cell Flow Panel
T Cell Exhaustion Flow Panel
Tfh-lin 1 Flow Panel
Tfh-lin 2 Flow Panel
Treg Flow Panel
X-Trial T Cell Flow Panel
RNA and DNA Protocols (Source: ITN Protocols)
Synthesizing cDNA Using ThermoScript RT-PCR Kit in 96 Plate Format
Synthesizing cDNA Using ThermoScript RT-PCR Kit
Assessing the quality of RNA using the Agilent 2100 Bioanalyzer
RNA and DNA Protocols (Source: IOWA State University Laboratory Protocols)
Golden Rice Case Study
Bt Corn vs the European Corn Borer (ECB) Activity
Chymosin Demonstration
DNA Extraction Bacteria
DNA Extraction Kiwi
DNA Extraction Onion
DNA Extraction fruit cup
DNA Extraction Smoothie
DNA Fingerprinting
DNA Transformation Ampicilin Resistance
DNA transformation Red Colony
Recombinant DNA Dual Antibiotic-Resistance Genes
DNA TRANSFORMATION Bacteria-Green Fluorescent Protein (GFP)
Evolution of Antibiotic Resistant Bacteria
High Sucrose Soybeans Invertase Teacher
High Sucrose Soybeans Invertase Student
HSSB-Thin Layer Chromatography Teacher
HSSB-Thin Layer Chromatography Student
Marker Assisted Selection of the K-Casein Allele
Pipettor Practice submitted by Jen Koenen, Hampton-Dumont High School, Hampton, Iowa
Plant Micropropagation Using African Violet Leaves
Plasmid Isolation and Analysis
Polymerase Chain Reaction PCR_Activity
Polymerase Chain Reaction PCRSimulation>
Polymerase Chain Reaction PCR-Alu-Sequence-Analysis-Beads
QuickStix™ Strip Test-Corn Leaf Tissue
QuickStix Strip Test-Corn Seed
QuickStix Strip Test-Roundup Ready Soybeans
Soybean Flavor Demonstration
Soy Drink Protocol
Cell Culture Protocols (Source: Bissell Lab, LBL-Programs)
1-HMT-3522 medium composition
2-HMT-3522 density
3-Splitting and passing HMT-3522 Cells
5-Freezing cells
9- On Top cells culture
EHS 3D Culture
Methyl cellulose
Anchorage Independence Assay using Methylcellulose
Invasion Assay
Soft Agar Assay for Colony Formation
Protein Lysis of 3D Cell Culture in Matrigel Imbedded or On Top using PBS
Isolation of T4 cells from xenograft tumors
Immunohistochemistry/Immunofluorescence Protocols (Source: Bissell Lab, LBL-Programs)
BrdU for human cells
Propidium Iodide Staining for DNA Ploidy
Occludin staining
In Situ Hybridization Method on Mammary Sections Using
Molecular and Biochemical Assays (Source: Bissell Lab, LBL-Programs)
FACS Analysis
RNA Extraction with TRIZOL
Chromatin immunoprecipitation assay
Mouse Mammary Gland Biology Protocols (Source: Bissell Lab, LBL-Programs)
Mouse mammary gland wholemounts
Primary Mammary Epithelial Cell Isolation
PCR and Sequencing Protocols (Souce: Barrick Lab, UT Austin)
Sequencing-Genotyping Primer Design>
Escherichia coli REL606 Genome Resources
Ordering Primers
Resuspending Primers
Standard PCR Polymerase Chain Reaction
Standard PCR Dpn I digestion
Overlap PCR
Agarose Gel Electrophoresis
DNA Concentration Determination
Sanger DNA Sequencing
16S rRNA Sequencing to Identify Unknown Microbes
Megaprimer whole plasmid cloning
Genetic Modification and Cloning Protocols (Souce: Barrick Lab, UT Austin)
Site-directed mutagenesis
Transformation Electrocompetent Cell
Transformation Chemically Competent Cells
Transformation General Conjugation
Assessing Transformation Efficiency
UV mutagenesis of Bacteria
Selecting Arabinose Marker Revertants
Colony Transformation
Restriction Enzyme Cloning
Gene Gorging Marker Mutations
Gene Gorging Evolved Alleles
FLP-FRT Recombination
Genome Modification
Gene replacement using pKOV vector
Gibson Cloning Assembly
Lambda red mediated ssDNA gene modification
pSLTS genomic editing for E coli
Golden Gate Assembly
Bee Microbiome Toolkit
P1 Transduction
Mutation Rates, Growth Rates and Evolutionary Stability (Souce: Barrick Lab, UT Austin)
Fluctuation Tests
Growth Rates using R studio
Evolutionary Stability of Fluorescent Protein or Chromoprotein Expression
Phage Lysate Preparation
Measuring Phage Titers
Measuring Adsorption Rate of T7 Phage
Measuring Lysis Timing of T7 Phage
Measuring Burst Size of T7 Phage
Genome Resequencing and Mutation Validation (Souce: Barrick Lab, UT Austin)
Genomic DNA Isolation
Targeted Sequencing of a Specific Gene
Mutation Rates from Genome Resequencing
Next-generation Sequencing Illumina library preparation
Experimental Evolution Protocols (Souce: Barrick Lab, UT Austin)
Competitive Fitness Assays
Ribose Operon Deletions
Mutation Accumulation for Microsatellite Strains
Bacterial Mutation Accumulation Experiments
Using Emulsions to Select by Yield
Flow Cytometry and Cell Sorting Protocols (Souce: Barrick Lab, UT Austin)
Generic Flow Cytometry guidelines
Nucleic Acid Preparation, RNA-Seq, qPCR Protocols (Souce: Barrick Lab, UT Austin)
Ethanol Precipitation
Bacterial RNA isolation
Plant RNA isolation
RNAseq Library Preparation
RT-qPCR for differential gene expression
qPCR for plasmid copy number
Denaturing Formaldehyde Gels for Verifying RNA size
Polyacrylamide Gel Electrophoresis
Protein Expression and Purification (Souce: Barrick Lab, UT Austin)
Large-scale Protein Expression in E. Coli
Running an SDS-PAGE Gel
Purifying 6xHis-Tagged Proteins from E. Coli
in vitro Enzyme Activity Assays (Souce: Barrick Lab, UT Austin)
Measuring transcription in vitro Using Broccoli and Spinach Fluorescent RNA Aptamers
Intracellular ROS detection
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