0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

Protein Running Buffers

Product#: ProteinRunningBuffers
$0.00

Select See the table below

  • See the table below

Availability:
Ships in 24 hours

verified Filtered 0.1 µm × 2 · 0.04 µm × 2 · Sterile-Environment Fill

Protein Running Buffers

This is a category page, not a product page — pick the buffer system that matches your gel and protein size range, then click View to open that product page. The chemistry reference, applications, and FAQ follow below.

Protein Running Buffers — Catalog · 12 Catalog Entries · 6 Products
This page is not a product page — click View on a row to open that product page.
Name Cat. No. pH Size Product Page
Tris-Tricine SDS Running Buffer DCP-TTSDSRB1X 8.3 500 ml Viewarrow_forward
Tris-Tricine SDS Running Buffer DCP-TTSDSRB1X 8.3 1000 ml Viewarrow_forward
Tris-Glycine SDS Buffer DCP-TGSB1X 8.3 500 ml Viewarrow_forward
Tris-Glycine SDS Buffer DCP-TGSB1X 8.3 1000 ml Viewarrow_forward
Tris-Glycine Non-Denaturing Buffer [1X] DCP-TGNDRB1X 8.3 500 ml Viewarrow_forward
Tris-Glycine Non-Denaturing Buffer [1X] DCP-TGNDRB1X 8.3 1000 ml Viewarrow_forward
Tris-Glycine Non-Denaturing Buffer [10X] DCP-TGNDRB10X 8.3 500 ml Viewarrow_forward
Tris-Glycine Non-Denaturing Buffer [10X] DCP-TGNDRB10X 8.3 1000 ml Viewarrow_forward
MES SDS Running Buffer DCP-MESSDSRB1X 7.3 500 ml Viewarrow_forward
MES SDS Running Buffer DCP-MESSDSRB1X 7.3 1000 ml Viewarrow_forward
MOPS SDS Running Buffer DCP-MOPSSDSRB1X 7.7 500 ml Viewarrow_forward
MOPS SDS Running Buffer DCP-MOPSSDSRB1X 7.7 1000 ml Viewarrow_forward

Not sure which system? Compare the buffer chemistries · See applications by buffer · Read the FAQ

Category Snapshot

What these buffers are for

Protein running buffers are essential components in protein gel electrophoresis systems, used to separate proteins based on their size and charge. These buffers create the electrical conductivity necessary for protein migration through the gel matrix. Different buffer systems are designed for various gel types and protein size ranges (e.g., Tris-glycine for standard SDS-PAGE, MOPS or MES for Bis-Tris gels).

  • Six products across four chemistries. Tris-Tricine, Tris-Glycine, MES, and MOPS — the last two being the Bis-Tris gel pairing the source names explicitly.
  • Denaturing and non-denaturing are both stocked. Four products carry SDS in the name; the Tris-Glycine Non-Denaturing Buffer is offered at 1X and 10X.
  • pH is specified per product. 8.3 for the Tris-based systems, 7.3 for MES, 7.7 for MOPS — a spread that reflects the gel each is built for.
  • The buffer system follows the gel, not the protein alone. The source states that different buffer systems are designed for various gel types and protein size ranges.
  • Every product ships in two sizes. 500 ml and 1000 ml, across all six products.
  • Appearance. Clear, colorless liquid.
  • Sterility. Filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment.[a]
0.1 µm × 2 0.04 µm × 2 RUO
CATEGORY REFERENCE · PROTEIN RUNNING BUFFERS
The category at a glance — chemistries, pH, formats, and the filtration specification
  • Products in this category6
  • Catalog entries (Cat. No.)12
  • Buffer chemistriesTris-Tricine, Tris-Glycine, MES, MOPS
  • Denaturing (SDS) products4
  • Non-denaturing products2
  • pH range across the category7.3 – 8.3
  • Concentrations named[b]1X, 10X
  • Fill sizes500 ml, 1000 ml
  • AppearanceClear, colorless liquid
  • Total membrane passes[a]4

[a] The source states “Filtered 0.1 micron Twice and 0.04 micron once”; that wording is canonicalized to 0.04 micron twice under the governing ruleset before it is used anywhere on the page. Flagged for confirmation — see the filtration section.
[b] 10X is carried in the source name of one product. 1X is taken from the catalog numbers (all six end in 1X or 10X) and from the published product titles; four of the six source names carry no concentration token. Confirm against the Certificate of Analysis before use.

Why the Buffer System Matters

Six things that follow from the chemistry you pick

The source's own framing is that different buffer systems are designed for different gel types and protein size ranges. The cards below unpack what that means at the bench.

bolt

It carries the current

These buffers create the electrical conductivity necessary for protein migration through the gel matrix. Without a conductive medium in the tank there is no field, and the separation that follows — by size and charge — cannot begin.

grid_on

The gel decides the buffer

Tris-glycine is the standard pairing for conventional SDS-PAGE, while MOPS and MES are the pairings for Bis-Tris gels. That mapping comes straight from the source, and it is the first filter to apply before anything else on this page.

straighten

The buffer sets the size window

MES and MOPS run on the same Bis-Tris gel yet resolve different ranges, because MES has the lower pKa and migrates faster, which changes stacking and therefore separation range.[1,2] Same gel, different buffer, different part of the ladder in focus.

science

Operating pH is not cosmetic

This catalog spans pH 7.3 to 8.3 as supplied. The neutral-pH Bis-Tris systems were developed because the classical Laemmli system runs at a strongly basic operating pH, where residual unpolymerized acrylamide can react with cysteine and lysine residues.[1]

layers

SDS or no SDS changes the question

Four products in this catalog carry SDS and separate denatured polypeptides by size. The Tris-Glycine Non-Denaturing Buffer omits it, so proteins stay folded and separation reflects native charge, size, and shape together.

filter_alt

Particulates stay in contact all run

Every buffer here is filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment. A running buffer submerges the gel for the entire separation, so anything suspended in it is in contact with the matrix from the first volt to the last.

How to work down this catalog

Three questions, in order. First, which gel are you running — a conventional Tris-glycine gel, a Bis-Tris gel, or a tricine gel for small peptides? That eliminates most of the list. Second, denaturing or native? That picks between the SDS products and the non-denaturing one. Third, if you landed on a Bis-Tris gel, MES or MOPS — the smaller your protein of interest, the stronger the case for MES.[1,2]

7.3 – 8.3
pH span across the six products as supplied
12
catalog entries — 6 products × 2 fill sizes
Filtration & Handling

Four membrane passes before the buffer is filled

The source states one sterility specification for the whole category: filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment. The four stages below are that statement, in order.

  1. 1

    0.1 µm Pre-filtration I

    First pass through a 0.1 micron membrane, removing bulk particulates carried in from raw materials and dissolution.

  2. 2

    0.04 µm Pre-filtration II

    Second pass at 0.04 micron, a rating below the 0.1 micron stage that precedes it.

  3. 3

    0.1 µm Sterile-filtration I

    Repeat 0.1 micron pass, performed within the sterile environment in which the buffer is filled.

  4. 4

    0.04 µm Sterile-filtration II — final polish

    Final 0.04 micron polishing pass immediately before fill, in the same sterile environment.

Why this matters for a tank buffer specifically

A running buffer is not added to the sample — it submerges the gel and stays there for the whole separation, often for hours. Anything suspended in it has that entire time in contact with the matrix and with the proteins migrating through it. Four membrane passes ending at 0.04 micron are aimed at that exposure. Appearance as supplied is a clear, colorless liquid; a cloudy or tinted bottle is worth investigating before it goes into a tank.

4
membrane passes before fill — 0.1 micron twice, then 0.04 micron twice
0.04 µm
rating of the final polishing membrane stated by the source
Quadruple-stage filtration diagram for Diagnocine Protein Running Buffers: 0.1 micron pre-filtration twice and 0.04 micron sterile filtration twice, four membrane passes before sterile-environment fill of Tris-Glycine SDS, Tris-Tricine SDS, MES SDS and MOPS SDS protein electrophoresis running buffers.
Figure 1. Quadruple-stage architecture: two 0.1 micron passes and two 0.04 micron passes, the final polish immediately before sterile-environment fill. © Diagnocine® — Protein Running Buffers
On the filtration wording. The source reads “Filtered 0.1 micron Twice and 0.04 micron once”. The governing ruleset canonicalizes that exact phrasing to “0.04 micron twice” before it is used anywhere, and both readings resolve to the same four-stage architecture and the same figure. The rewrite is recorded here and flagged for confirmation against the real process rather than silently applied.
Chemistry Reference

The four buffer systems in this catalog

Product names, catalog numbers, and pH values are from the source. The gel pairing for Tris-glycine, MOPS, and MES is stated by the source. The final column is published third-party guidance on separation range, cited below — it is not a Diagnocine performance specification, and the source gives no size range for any product.

Buffer system Products pH Denaturing? Gel pairing Published separation guidance[1,2]
Tris-Glycine SDS Tris-Glycine SDS Buffer (DCP-TGSB1X) 8.3 check_circle Yes Standard SDS-PAGE, as stated by the source The classical Laemmli system; the general-purpose default for denaturing protein gels
Tris-Glycine, non-denaturing Tris-Glycine Non-Denaturing Buffer [1X] (DCP-TGNDRB1X)
Tris-Glycine Non-Denaturing Buffer [10X] (DCP-TGNDRB10X)		 8.3 cancel No Native-PAGE on Tris-glycine gels Proteins stay folded, so mobility reflects native charge, size, and shape together
Tris-Tricine SDS Tris-Tricine SDS Running Buffer (DCP-TTSDSRB1X) 8.3 check_circle Yes Tricine gel systems Tricine replaces glycine as the trailing ion; the system developed to resolve small proteins and peptides[3]
MES SDS MES SDS Running Buffer (DCP-MESSDSRB1X) 7.3 check_circle Yes Bis-Tris gels, as stated by the source Recommended for small- to medium-sized proteins; MES has the lower pKa, so it migrates faster than MOPS
MOPS SDS MOPS SDS Running Buffer (DCP-MOPSSDSRB1X) 7.7 check_circle Yes Bis-Tris gels, as stated by the source Recommended for medium- to large-sized proteins; proteins run slower than in MES
Why MES and MOPS give different results on the same gel. They differ in pKa. MES has the lower value, which makes the MES buffer the faster of the two; that difference in ion migration changes how proteins stack as they enter the resolving gel, and stacking is what sets the separation range.[1,2] So running the identical Bis-Tris gel in MES rather than MOPS does not simply speed the run up — it moves the window of the ladder that is well resolved. Published ranges are quoted by gel manufacturers for their own gels; check the range published for the gel you are actually using rather than assuming a universal figure. This clarification is an addition beyond the source description.
Applications by Buffer

Which buffer for which gel

One tab per buffer system; the two Tris-Glycine Non-Denaturing concentrations share a tab because the chemistry is identical. The first bullet in each panel is the source description's own statement of use; the remaining bullets are workflow-level notes drawn from the cited references and can be removed independently.

DCP-TGSB1X · pH 8.3 · 500 ml / 1000 ml
  • Separating proteins based on their size and charge, by creating the electrical conductivity necessary for protein migration through the gel matrix
  • Standard SDS-PAGE on Tris-glycine gels — the pairing the source names explicitly
  • Routine expression checks, lysate profiling, and fraction analysis during purification
  • Western blot workflows built on the conventional Laemmli gel format
DCP-TGNDRB1X and DCP-TGNDRB10X · pH 8.3 · 500 ml / 1000 ml
  • Separating proteins based on their size and charge, by creating the electrical conductivity necessary for protein migration through the gel matrix
  • Native-PAGE, where the protein must stay folded and mobility reflects native charge, size, and shape together
  • In-gel activity work, where a denaturing run would destroy the readout
  • Available at 1X for occasional runs and 10X for labs that would rather dilute per run than stock working-strength bottles
DCP-TTSDSRB1X · pH 8.3 · 500 ml / 1000 ml
  • Separating proteins based on their size and charge, by creating the electrical conductivity necessary for protein migration through the gel matrix
  • Tricine gel systems, where tricine replaces glycine as the trailing ion[3]
  • Small proteins and peptides, the separation problem the tricine system was developed to address[3]
  • Work where bands at the bottom of a conventional glycine gel are too compressed to resolve
DCP-MESSDSRB1X · pH 7.3 · 500 ml / 1000 ml
  • Separating proteins based on their size and charge, by creating the electrical conductivity necessary for protein migration through the gel matrix
  • Bis-Tris gels — the pairing the source names explicitly
  • Small- to medium-sized proteins, the range published guidance assigns to MES[1,2]
  • Faster runs on a Bis-Tris gel, since MES has the lower pKa and migrates faster than MOPS[1,2]
DCP-MOPSSDSRB1X · pH 7.7 · 500 ml / 1000 ml
  • Separating proteins based on their size and charge, by creating the electrical conductivity necessary for protein migration through the gel matrix
  • Bis-Tris gels — the pairing the source names explicitly
  • Medium- to large-sized proteins, the range published guidance assigns to MOPS[1,2]
  • Runs where the slower migration in MOPS spreads the upper part of the ladder further apart[1,2]
Denaturing or Native, 1X or 10X

The two format decisions

Once the gel has narrowed the chemistry, two questions remain. Neither is about quality — both are about what you are asking the gel and how much buffer you go through.

  Denaturing (SDS) Non-denaturing (native)
Products in this catalog Tris-Glycine SDS Buffer
Tris-Tricine SDS Running Buffer
MES SDS Running Buffer
MOPS SDS Running Buffer		 Tris-Glycine Non-Denaturing Buffer [1X]
Tris-Glycine Non-Denaturing Buffer [10X]
Protein state during the run Unfolded and detergent-coated Folded, in its native state
What migration reflects Primarily size Native charge, size, and shape together
Useful for apparent molecular-weight assignment check_circle Yes cancel Not directly
Useful for in-gel activity work cancel No check_circle Yes
pH as supplied 8.3 (Tris-Glycine, Tris-Tricine), 7.3 (MES), 7.7 (MOPS) 8.3
Concentrations offered 1X 1X and 10X — a 1000 ml bottle at 10X makes 10 litres of working buffer
Fill sizes 500 ml, 1000 ml 500 ml, 1000 ml
Shared across the category. Appearance: clear, colorless liquid. Sterility: filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment.[a] For Research Use Only (RUO) — not intended for clinical, diagnostic, or therapeutic use in humans.
FAQ

Frequently asked questions

The questions that come up most often when a bench protocol meets a purchase order.

Start with the gel. A conventional Tris-glycine gel takes the Tris-Glycine SDS Buffer for denaturing runs or the Tris-Glycine Non-Denaturing Buffer for native ones. A Bis-Tris gel takes MES SDS or MOPS SDS. A tricine gel takes the Tris-Tricine SDS Running Buffer. The source states this mapping directly: different buffer systems are designed for various gel types and protein size ranges.
The separation range changes. MES has the lower pKa, which makes it the faster of the two; that difference in ion migration alters how proteins stack entering the resolving gel, and stacking sets the range that resolves well. Published guidance assigns MES to small- to medium-sized proteins and MOPS to medium- to large-sized ones. Check the range published for the specific gel you are running rather than assuming a universal figure.
Those are the values the source specifies for each product as supplied, and they reflect the gel system each is built for. The Bis-Tris systems are deliberately near-neutral: the classical Tris-glycine system operates at a strongly basic pH, where residual unpolymerized acrylamide can react with cysteine and lysine residues on the proteins being separated. Running nearer neutral slows those reactions considerably.
When your bands of interest are small enough to be compressed at the bottom of a conventional glycine gel. The tricine system replaces glycine with tricine as the trailing ion, and it was developed specifically to extend clean separation down into the small-protein and peptide range. If you are resolving anything in that region, it is the buffer this catalog offers for it.
SDS. The four SDS buffers keep proteins unfolded and detergent-coated, so migration tracks size and you can read apparent molecular weight against a ladder. The Tris-Glycine Non-Denaturing Buffer omits SDS, so proteins enter the gel folded and mobility reflects native charge, size, and shape together. That is what makes in-gel activity readouts possible, and it is why both are stocked.
It depends on volume, not quality. Both are the same chemistry at pH 8.3, supplied in 500 ml and 1000 ml. A 1000 ml bottle at 10X makes 10 litres of working buffer, which is a large reduction in shelf and cold-storage volume if you run native gels regularly. If you run them occasionally, the 1X bottle saves the dilution step. Note that the 10X option is offered only for the non-denaturing buffer in this catalog.
Every buffer in this category is filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment — four membrane passes, the last a 0.04 micron polish immediately before fill. A running buffer submerges the gel for the entire separation, so anything suspended in it is in contact with the matrix and the migrating proteins for hours rather than seconds. Appearance as supplied is a clear, colorless liquid.
Key References

Sources behind the cited notes

The source description carries no citation list. The entries below support only the statements marked with a superscript above. Where a source publishes no DOI, none is shown — none has been invented.

  1. Thermo Fisher Scientific. NuPAGE Bis-Tris and Bolt Bis-Tris Plus gels — technical guidance, and the accompanying NuPAGE Technical Guide. — Source of the Bis-Tris discontinuous buffer description: MES or MOPS serves as the trailing ion, the running buffer sits at pH 7.3 to 7.7 against a pH 6.4 gel buffer, and the combination gives an operating pH near 7. Also the basis for the note that the classical Laemmli system runs at a strongly basic pH where residual unpolymerized acrylamide can react with cysteine and lysine residues.
  2. Invitrogen / Thermo Fisher Scientific. NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer — product documentation. — MES SDS is recommended for small- to medium-sized proteins and MOPS SDS for medium- to large-sized proteins; MES has a lower pKa than MOPS, making the MES buffer faster, and the resulting difference in ion migration affects stacking and therefore the separation range. Published formulations are pH 7.3 (MES) and pH 7.7 (MOPS).
  3. Schägger, H., & von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry, 166(2), 368–379. — The origin of the tricine system, in which tricine replaces glycine as the trailing ion to extend clean separation to small proteins and peptides. doi:10.1016/0003-2697(87)90587-2
  4. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680–685. — The discontinuous Tris-glycine SDS system that standard SDS-PAGE running buffers are formulated for. doi:10.1038/227680a0
Buffer selection support. For help matching a running buffer to a gel type, a protein size range, or a native-versus-denaturing protocol — or for documentation requests — contact support@diagnocine.com. Ready to order? Back to the Protein Running Buffers catalog.

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X