Saline-Sodium Citrate Buffer [2X]
Cat No.: DCP-SSCB2X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Saline Sodium Citrate Buffer is used as a hybridization buffer for DNA and RNA in techniques like Southern blotting, Northern blotting, in situ hybridization, and DNA microarrays. It is used to control stringency during washing steps by adjusting salt concentration. Higher concentration (2X) allows less stringent washing conditions, while lower concentrations (0.5X) increase stringency and reduce non-specific binding.
Saline Sodium Citrate Buffer is used for denaturation to facilitate screening of DNA libraries. It is employed as a transfer buffer for blotting DNA or RNA from agarose or polyacrylamide gels onto nitrocellulose or nylon membranes during Southern blotting or Northern blotting procedures. High concentrations (i.e., 20X) are typically used for nitrocellulose membranes to prevent loss of smaller DNA fragments. High concentrations (i.e., 20X) can also be used to prevent the drying of agarose gels during vacuum transfer processes.
* Please inquire about other concentrations.
Advantages:
- It helps to control stringency in hybridization techniques. Versatility in nucleic acid applications include Northern and Southern transfer protocols and nucleic acid preparations. It's a standard reagent in Southern and Northern hybridization procedures.
- It helps to maintain the stability of DNA and RNA during hybridization and acts as a buffering agent to control acidity, maintaining a stable pH for biological reactions. It also demonstrates good stability, which is crucial for consistent experimental results.
- Lacks toxicity, making it safe for use in biological system; with a pH of 7.0 ± 0.05 at 25°C, SSC buffer operates in the optimal range for most biological reactions (between 6.0 and 8.0).
- Has minimal salt effects due to its ionic composition and minimal participation in biological reactions, and prevents drying of agarose gels.
- It is used to transfer DNA/RNA to membranes.In hybridization procedures, SSC buffer can be used to control the stringency of wash steps, allowing for optimization of specific vs. non-specific binding.
Applications:
- Nucleic Acid Hybridization: It is commonly used as a hybridization buffer for DNA and RNA, particularly in Northern and Southern blotting procedures.
- Antigen Retrieval: Sodium citrate buffer is highly utilized in immunohistochemistry (IHC) for heat-induced antigen retrieval (HIER). It helps break the protein cross-links formed during fixation, thereby improving antigen detection.
- Blot Transfer: The buffer is employed in transfer procedures for DNA and RNA, such as Southern and Northern blotting.
- In Situ Hybridization: It is specifically developed for in situ hybridization on brain sections, helping to control the stringency of hybridization.
- Nucleic Acid Preparation: The buffer is used in various nucleic acid preparation protocols.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
0.3 M |
| Sodium Citrate |
0.03 M |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
