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Gelatin Based Blocking Buffers

Gelatin Blocking Buffers are used in various immunoassays, such as Western blotting and ELISA, to block non-specific binding sites on membranes and microplates. They help improve the sensitivity and specificity of the assays by reducing background noise. These buffer contains 1% gelatin, which is derived from animal collagen. Gelatin is known for its ability to form a gel-like matrix, effectively covering potential non-specific binding sites. Gelatin is generally less effective at blocking non-specific binding compared to casein and BSA.They are more suitable for blocking protein-protein interactions rather than protein-surface interactions, which can result in higher background noise and decreased sensitivity in some assays. Gelatin does not cross-react with mammalian antibodies, making it a good choice for assays involving mammalian samples.

Storage: 4 ℃ 
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.

CUSTOMIZATION:  Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.