Nonfat milk based blocking buffers are commonly used reagents in Western blotting and other immunoassay techniques. The primary function of nonfat milk based blocking buffer is to reduce non-specific binding of antibodies to the membrane, thereby decreasing background noise and improving signal-to-noise ratio in Western blots and other immunoassays. These work well with a wide range of antibodies and detection methods. Nonfat milk often provides the lowest background, especially on nitrocellulose membranes. These products contain ProClin 300 which helps maintain the integrity of the solution, preventing microbial growth. ProClin 300 is more effective than sodium azide as a preservative. Sodium Azide interferes with enzymatic activities such as phosphatase/ Peroxidase used in blotting experiments, while ProClin 300 does not interfere and is compatible with enzymes and antibodies used in diagnostic assay.
Storage: 4 ℃
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.