0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

Citrate Buffer, Sterile [0.1X]

Product#: DCP-CB0.1X
$137.50
Availability:
Ships in 24 hours

ISO 13485 Certified Manufacturing

FluxMPS™ Citrate Buffer, Sterile [0.1X]

An MPS-grade, 0.1 M citrate buffer at pH 3.0, manufactured with quadruple-stage filtration for ultra-low particulate, mycoplasma-safe purity. Sterile, ultrapure, and DNase/RNase-tested, it is built for immunohistochemistry, nucleic acid work, and microfluidic channel-safe research applications.

  • Quadruple-stage filtration: 0.1 µm membrane filtered twice and 0.04 µm membrane filtered twice for ultra-low particulate purity
  • Precise, defined formulation: pH 3.0, 0.1 M citrate buffer (0.1X)
  • Sterile and ultrapure — filtered and packaged in a sterile environment
  • No detectable DNase or RNase activity after 18-hour incubation at room temperature
  • Manufactured with ultrapure Type 1 water (18.2 MΩ·cm)
  • Produced under ISO 13485-certified, CE-approved facilities
  • Supports IHC antigen retrieval, RNA extraction, DNA FISH, enzymatic reactions, and chromatography
  • Custom concentrations, pH, and additive formulations available on request
SKU: DCP-CB0.1X | 12352204 · Nucleic acids | Buffers & Water
Citrate Buffer, Sterile [0.1X] — 500 mL
  • pH3.0
  • Molarity / Concentration0.1 M
  • Dilution Factor0.1X
  • AppearanceClear, Colorless Liquid
  • Filtration0.1 µm membrane x2, 0.04 µm membrane x2
  • SterilitySterile, filtered in a sterile environment
  • DNase ActivityNone detected (18 hr, RT)
  • RNase ActivityNone detected (18 hr, RT)
  • Storage4°C
  • Shelf Life18 months
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard buffers fail

Conventional 0.22 µm-filtered buffers can carry subvisible particulates, drift in pH, and permit mycoplasma-sized organisms to persist — all of which accumulate in microchannels, elevate assay background, and compromise reproducibility. FluxMPS™ Citrate Buffer is engineered against every one of these failure modes.

filter_alt

Microchannel-safe purity

Quadruple-stage 0.1 µm / 0.04 µm filtration removes fine particulates that a single-pass 0.22 µm filter can miss, protecting delicate microfluidic channels and valves.

target

Precise, stable pH

Formulated to a defined 0.1 M molarity at pH 3.0, and designed to maintain relatively stable pH across a range of temperatures, suiting protocols with temperature changes.

water_drop

Ultrapure-grade water

Manufactured with ultrapure Type 1 water (18.2 MΩ·cm), consistent with USP <85> water-quality practice.

visibility

Low background for imaging & assays

As a citrate-based antigen retrieval buffer it is known to enhance staining intensity without increasing background signal, improving signal-to-noise in IHC and imaging workflows.

science

Defined, traceable composition

Tested and confirmed free of detectable DNase and RNase activity after 18-hour incubation, with all packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center.

tune

Customization on demand

Other concentrations, pH values, and additions of chemicals, compounds, proteins, or supplements can be arranged — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

DCP-CB0.1X is filtered with a 0.1-micron membrane twice and a 0.04-micron membrane twice, all within a sterile filling environment, delivering an ultra-low particulate, mycoplasma-safe buffer for cell and molecular biology work.

  1. 1

    0.1 µm Pre-filtration I

    Removes large particulates and aggregates, extending the working life of downstream filtration stages.

  2. 2

    0.04 µm Pre-filtration II

    Retains fine particulates and bioburden. The smallest mycoplasma species can be as small as approximately 0.2 micron, so this stage is a key line of defense against mycoplasma contamination.

  3. 3

    0.1 µm Sterile-filtration I

    A second 0.1-micron pass adds redundancy ahead of final polishing.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    A second 0.04-micron pass delivers the final polish, completed in a sterile environment to prevent mycoplasma contamination.

Performance vs. conventional buffer

Sequential 0.1 µm and 0.04 µm filtration, applied twice each, removes finer particulates and mycoplasma-sized contaminants than a single 0.22 µm pass used in conventional buffer solutions.

0.04 µm
Final filtration stage
4
Total filtration stages
Filtered and packaged under sterile conditions consistent with USP <71> sterility testing practice; no DNase or RNase activity was detected after 18-hour incubation at room temperature.
FluxMPS DCP-CB0.1X quadruple-stage 0.1 micron and 0.04 micron filtration diagram for sterile citrate buffer used in organ-on-a-chip and microfluidic research at Diagnocine
Figure 1. Quadruple-stage filtration architecture (0.1 µm x2, 0.04 µm x2) applied to DCP-CB0.1X.
© Diagnocine® — DCP-CB0.1X
Applications

Where DCP-CB0.1X performs

Citrate buffer is a versatile reagent across immunohistochemistry, nucleic acid preparation, molecular cytogenetics, and analytical chemistry, with formulation choices tuned to protect sensitive downstream workflows.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion systems and robotic liquid handlers, an optional 0.01 µm (10 nm) ultra-filtered grade of this buffer can be arranged to further reduce particulate load on valves, sensors, and long-duration perfusion loops.

  • Total Particulate Exclusion: Finer filtration reduces the risk of particulate accumulation in automated fluidic pathways.
  • Valve & Sensor Protection: Lower particulate load helps protect precision valves and in-line sensors from fouling.
  • Extended Perfusion Stability: Cleaner input buffer supports longer unattended perfusion runs.

Inquiry Required: The 0.01 µm ultra-filtered grade is available on request — contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

MPS-grade purity suited to organ-on-a-chip and lab-on-a-chip workflows where microchannel integrity matters.

OoCToCBoCLoCMPS
Immunohistochemistry

Heat-Induced Epitope Retrieval (HIER)

Breaks protein cross-links formed during formalin fixation to unmask antigens and epitopes on FFPE tissue sections, enhancing antibody staining intensity with a very low background.

FFPEIHCHIERAntigen Retrieval
Nucleic Acid Prep

RNA Extraction Support

Helps prevent base hydrolysis during RNA extraction protocols.

RNA ExtractionHydrolysis Prevention
Molecular Cytogenetics

DNA Fluorescent In Situ Hybridization

Used in DNA FISH workflows as part of probe hybridization and detection protocols.

DNA FISHCytogenetics
Analytical Chemistry

Enzymatic Reactions & Chromatography

Its chelating action on calcium ions and stable low pH support use in enzymatic reaction buffers and chromatography workflows.

Enzymatic AssaysChromatography
Live-Cell Imaging

Microscopy & Optical Sensing

Low-background performance supports high signal-to-noise imaging and optical detection applications.

ConfocalBiosensorsTEER
Technical Specifications

Specifications at a glance

Measured and declared values for DCP-CB0.1X, Citrate Buffer, Sterile [0.1X].

Physical & Chemical Parameters
Parameter Specification
Formulation / Composition Citrate Buffer, 0.1 M (0.1X)
Appearance Clear, Colorless Liquid
pH USP <791> 3.0
Molarity / Concentration 0.1 M
Dilution Factor 0.1X
Sterility, Purity & Safety Parameters
Parameter Specification
Sterility USP <71> Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment
DNase Activity None detected after 18-hour incubation with plasmid DNA at room temperature
RNase Activity None detected after 18-hour incubation with ribosomal RNA at room temperature
Water Quality Ultrapure Type 1 water (18.2 MΩ·cm)
Storage, Handling & Logistics
Parameter Specification
Storage Temperature 4°C
Shelf Life 18 months
pH Stability Maintains relatively stable pH over a range of temperatures
Raw Materials & Regulatory Traceability
Parameter Specification
Raw Material Grade Ultrapure
Manufacturing QMS ISO 13485 ISO 13485-certified, CE-approved supplier facilities
Traceability Final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center
Production Method Customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA
Intended Use Research Use Only (RUO)
Formulation

Full composition

Citrate buffer system formulated to 0.1 M at pH 3.0, prepared and packaged under sterile, quadruple-stage filtered conditions.

Component CAS Number Concentration
Citrate Buffer (Sodium Citrate / Citric Acid System)   0.1 M
Other concentrations, pH values, and additions of chemicals, compounds, proteins, or supplements can be arranged — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

DCP-CB0.1X is manufactured under a formal quality system, with final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center.

verified

ISO 13485:2016 QMS

Manufactured under ISO 13485-certified and CE-approved supplier facilities.

water_drop

Ultrapure Type 1 Water

Formulated with ultrapure Type 1 water (18.2 MΩ·cm).

biotech

Sterile Fill & Finish

Filtered and packaged in a sterile environment to prevent mycoplasma contamination.

assignment

Micro-Batch Precision

Customization and assembly completed at DiagnoCine Precision, Totowa, New Jersey, USA.

DNase / RNase Testing

No DNase or RNase activity detected after 18-hour incubation with plasmid DNA or ribosomal RNA at room temperature.

Sterility Methodology USP <71>

Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment.

pH Methodology USP <791>

Formulated and controlled to pH 3.0.

Documentation / CoA

A Certificate of Analysis can be requested for this lot.

CoA documentation is available upon request — contact support@diagnocine.com.
Product Comparison

How DCP-CB0.1X compares

A side-by-side view of DCP-CB0.1X against conventional single-pass filtered citrate buffer.

Parameter DCP-CB0.1X (FluxMPS™) Conventional 0.22 µm buffer Standard alternative buffer
Defined pH & Molarity (pH 3.0, 0.1 M) check_circle cancel cancel
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 1 1
Sterility methodology USP <71> check_circle cancel cancel
DNase / RNase tested absence check_circle cancel cancel
Ultrapure Type 1 water base check_circle cancel cancel
ISO 13485-certified, CE-approved manufacturing check_circle cancel cancel
Microfluidic channel compatibility check_circle cancel cancel
Stable pH across temperature range check_circle cancel cancel
Custom formulation available check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about DCP-CB0.1X, Citrate Buffer, Sterile [0.1X].

Yes. Its quadruple-stage 0.1 µm / 0.04 µm filtration and sterile, ultrapure formulation are designed to be microchannel-safe for OoC, ToC, BoC, LoC, and other MPS-based platforms.
DCP-CB0.1X is filtered with a 0.1-micron membrane twice and a 0.04-micron membrane twice in a sterile environment. Sequential filtration at these two pore sizes removes finer particulates and mycoplasma-sized organisms (the smallest of which can be about 0.2 micron) that a single 0.22 µm pass may not fully exclude.
DCP-CB0.1X is formulated at pH 3.0 and 0.1 M molarity (0.1X). Ionic strength is not independently specified. Other concentrations, pH values, and chemical, compound, protein, or supplement additions can be arranged — contact support@diagnocine.com.
A specific measurement temperature for the pH 3.0 value is not stated. The buffer is designed to maintain a relatively stable pH over a range of temperatures, making it suitable for applications involving temperature changes, and should be stored at 4°C.
Yes. Please inquire if other concentrations, additions of chemicals, compounds, proteins, or supplements, different pH, or other modifications are needed — contact support@diagnocine.com.
Endotoxin level is not part of the standard specification published for this product. Testing under USP <85> Bacterial Endotoxins Test methodology can be arranged upon request — contact support@diagnocine.com.
Yes. A CoA covering pH, appearance, sterility, and DNase/RNase test results can be requested — contact support@diagnocine.com.
Scientific References

Supporting literature

Curated literature relevant to citrate buffer use in antigen retrieval, nucleic acid work, microfluidics, and sterility control.

  1. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining. J Histochem Cytochem. doi:10.1177/002215549104100601
  2. Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem. doi:10.1177/002215549704501001
  3. Ingber DE. Reverse engineering human pathophysiology with organs-on-chips. Cell. doi:10.1016/j.cell.2016.05.021
  4. Huh D, Matthews BD, Mammoto A, et al. Reconstituting organ-level lung functions on a chip. Science. doi:10.1126/science.1188302
  5. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. doi:10.1038/nbt.2989
  6. Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures. Curr Protoc Mol Biol. doi:10.1002/0471142727.mb2404s106
  7. Levine AJ. Buffers and pH control in molecular biology reactions. Methods Enzymol. doi:10.1016/S0076-6879(09)64001-2
  8. Speirs V, Ellison DW. Citrate buffer-based antigen retrieval protocols for immunohistochemistry. Methods Mol Biol. doi:10.1007/978-1-60761-950-5_9

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X