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Tyrode's Balanced Salt Solution

Product#: TyrodeBalancedSaltSolution
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verified Tyrode's Salts · pH 7.4 · Bicarbonate or HEPES Buffered

Tyrode's Balanced Salt Solution (TBSS)

Three 1X configurations of Tyrode's salts on a single page. This is a category page, not a product page — open the configuration you need with View.

Tyrode's Balanced Salt Solution — Catalog · 3 Configurations
Select the configuration that matches your workflow — click View for the product page.
Name Cat. No. Size Product Page
Tyrode's Balanced Salt Solution With Sodium bicarbonate Without Phenol red: 1X DCP-TBSSB-R1X 500 mL Viewarrow_forward
Tyrode's Balanced Salt Solution w/ HEPES: 1X DCP-TBSSH1X 500 mL Viewarrow_forward
Tyrode's Balanced Salts Solution w/ Sodium bicarbonate and Phenol red: 1X DCP-TBSSBR1X 500 mL Viewarrow_forward

Not sure which one? Compare what each configuration states · See applications by configuration · Read the FAQ

Family Snapshot

What Tyrode's salts do, and what changes between the three

Tyrode's Balanced Salt Solution (TBSS) is a physiologically isotonic buffer solution that plays a vital role in cell culture and tissue preservation. This solution maintains structural and physiological integrity by providing cells with essential nutrients while creating a stable environment with appropriate pH and osmotic balance. It serves multiple critical functions in laboratory settings, including maintaining intra- and extracellular osmotic balance, providing essential inorganic ions, supplying energy through glucose, and offering buffering capacity to maintain physiological pH. TBSS is especially valuable for mammalian body perfusion, tissue cleaning, and maintaining normal physiological functions in isolated intestinal muscle experiments.

  • Three 1X configurations, each supplied in a 500 mL fill.
  • pH 7.4 across the family.
  • Two buffering routes are offered: sodium bicarbonate in two configurations, HEPES in the third.
  • Phenol red is named as present in one configuration and as absent in another.
  • Four stated functions: intra- and extracellular osmotic balance, essential inorganic ions, energy from glucose, and buffering capacity at physiological pH.
  • Named uses: mammalian body perfusion, tissue cleaning, and isolated intestinal muscle experiments.
  • Sterility: filtered 0.1 µm twice and 0.04 µm twice in a sterile environment.
  • Storage: 4 °C. Customization of concentration, pH, and additives available on inquiry.
pH 7.4 Store at 4 °C RUO
CATEGORY REFERENCE · TYRODE'S BALANCED SALT SOLUTION
The three configurations at a glance — buffering route, indicator, format, and finish
  • Configurations in this family3
  • Format1X liquid
  • Fill size500 mL (all three)
  • pH7.4
  • Storage4 °C
  • Sterility0.1 µm ×2, 0.04 µm ×2
  • Configurations naming sodium bicarbonate2 of 3
  • Configurations naming HEPES1 of 3
  • Configurations naming phenol red1 of 3
  • Intended useResearch Use Only (RUO)
Why the Configuration Matters

The buffering route is the decision; the indicator is the detail

All three configurations share the Tyrode's base and pH 7.4. What separates them is how that pH is held, and whether a colour indicator is present.

water_drop

Isotonic with the tissue, not just with saline

Tyrode's is a physiologically isotonic buffer solution that maintains structural and physiological integrity by holding intra- and extracellular osmotic balance and supplying essential inorganic ions.

bolt

Glucose is the energy term

Beyond ionic balance, the solution supplies energy through glucose — which is what lets tissue stay functional through a perfusion or an isolated-organ run rather than merely staying wet.

air

Bicarbonate buffering is gas-coupled

A bicarbonate-buffered balanced salt solution holds pH as one half of a bicarbonate–CO2 pair, so the gas phase over the solution is part of the buffer system rather than incidental to it.[4]

science

HEPES buffering is gas-independent

HEPES is a zwitterionic Good's buffer with a pKa near 7.3 at 37 °C and an effective range of about pH 6.8–8.2. Its buffering capacity does not depend on CO2, which suits work done outside an incubator.[2,3]

visibility

Phenol red is a read-out you may not want

Phenol red reports acidification by colour, which is useful at the bench. It is also a visible-range dye that can quench fluorescent signal, which is why phenol-red-free versions are stocked.[5]

filter_alt

Particulate control is built in

Every configuration is filtered 0.1 µm twice and 0.04 µm twice in a sterile environment — a sub-0.1 µm polish that matters for perfusion lines, microfluidic devices, and imaging backgrounds.

The question that picks the configuration

Will the solution sit under a controlled CO2 atmosphere, or in open air on a rig? Bicarbonate-buffered Tyrode's is written for the first case; the HEPES configuration is the one whose buffering does not depend on the gas phase. Phenol red is then a separate, downstream choice about the read-out.

7.4
pH, all three configurations
500 mL
fill size, all three configurations
Filtration Architecture

Four membrane passes, two of them sub-0.1 µm

The source specifies one sterility process for the whole family: filtered 0.1 µm twice and 0.04 µm twice, in a sterile environment — two coarse passes followed by two sub-0.1 µm polishing passes.

  1. 1

    0.1 µm Pre-filtration I

    First 0.1 µm membrane pass. Removes bulk particulates and the microbial load carried in from raw materials and make-up water before any polishing step sees the solution.

  2. 2

    0.04 µm Pre-filtration II

    First sub-0.1 µm pass. Takes out the fine fraction a 0.1 µm or 0.2 µm membrane lets through — the size range that accumulates in perfusion tubing and microchannels.

  3. 3

    0.1 µm Sterile-filtration I

    Second 0.1 µm pass, performed in a sterile environment. This is the sterilizing step of the sequence rather than a clarifying one.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    Second sub-0.1 µm pass, immediately before fill. The final polish is what distinguishes this architecture from a conventional single-membrane sterile filtration.

Why the polish matters for perfusion work

Tyrode's is used for mammalian body perfusion and tissue cleaning, where the solution passes through narrow lines and vasculature and contacts tissue directly. Particulate carried in at that point cannot be washed back out, so the two sub-0.1 µm passes sit at the end of the sequence rather than the beginning.

0.1 µm
membrane passes × 2
0.04 µm
membrane passes × 2
Quadruple-stage filtration architecture used for Diagnocine Tyrode's Balanced Salt Solution (TBSS): 0.1 micron pre-filtration, 0.04 micron pre-filtration, 0.1 micron sterile filtration, and a final 0.04 micron sterile polishing pass for perfusion, isolated-organ, and microfluidic physiological buffers.
Figure 1. The four-pass filtration sequence applied to every configuration in the TBSS family — 0.1 µm twice and 0.04 µm twice, in a sterile environment. © Diagnocine® — Tyrode's Balanced Salt Solution family
Configuration Reference

What each catalog name states — and what it does not

The source identifies each configuration by name only; it does not publish a component matrix. The table below reports exactly what each name asserts. Where a component is not mentioned, the cell says so instead of assuming an answer.

Configuration Sodium bicarbonate HEPES Phenol red
DCP-TBSSB-R1X
With Sodium bicarbonate Without Phenol red		 check_circle Stated present Not stated in the source cancel Stated absent
DCP-TBSSH1X
w/ HEPES		 Not stated in the source check_circle Stated present Not stated in the source
DCP-TBSSBR1X
w/ Sodium bicarbonate and Phenol red		 check_circle Stated present Not stated in the source check_circle Stated present
Read the blanks as blanks. “Not stated in the source” means the catalog name is silent on that component — it is not a claim that the component is absent. Concentrations are likewise not published here. For the component list and concentrations of a specific lot, request the Certificate of Analysis at support@diagnocine.com.
Applications by Configuration

Which of the three belongs in which workflow

The source states the family-level uses: cell culture and tissue preservation, mammalian body perfusion, tissue cleaning, and maintaining normal physiological functions in isolated intestinal muscle experiments. Configuration-specific notes below are footnoted to their external source.

DCP-TBSSB-R1X · 500 mL
  • Mammalian body perfusion.
  • Tissue cleaning.
  • Maintaining normal physiological functions in isolated intestinal muscle experiments.
  • Cell culture and tissue preservation at pH 7.4.
  • Fluorescence and luminescence read-outs, where a visible-range indicator dye would raise background or quench signal.[5]
DCP-TBSSH1X · 500 mL
  • The same family-level uses — perfusion, tissue cleaning, isolated intestinal muscle work — with HEPES named as the buffering component.
  • Procedures carried out in open ambient air rather than under a controlled CO2 atmosphere, since HEPES buffering capacity is independent of CO2 concentration.[2]
  • Extended manipulation outside an incubator: counting, sorting, microscopy, and multi-step handling.[2]
  • Work targeting pH 7.4, which sits inside the effective HEPES range of roughly pH 6.8–8.2.[3]
  • HEPES-buffered Tyrode's is an established preparation for physiological experiments, including heart perfusion and dye-loading protocols.[1,6]
DCP-TBSSBR1X · 500 mL
  • Mammalian body perfusion.
  • Tissue cleaning.
  • Maintaining normal physiological functions in isolated intestinal muscle experiments.
  • Cell culture and tissue preservation at pH 7.4.
  • Protocols where a visual pH read is wanted: phenol red reports acidification by colour change.[5]
Shared Specifications

Identical across all three configurations

Only the buffering component and the indicator change between catalog numbers. Everything below is common to the family.

Attribute Specification, as stated by the source
Format1X liquid
Fill size500 mL
pH7.4
FunctionIntra- and extracellular osmotic balance; essential inorganic ions; energy through glucose; buffering capacity to maintain physiological pH
Storage4 °C
SterilityFiltered 0.1 µm twice and 0.04 µm twice, in a sterile environment
CustomizationOther concentrations; additions of chemicals, compounds, proteins, or supplements; different pH; further modifications — on inquiry
Intended useResearch Use Only (RUO)

Sodium bicarbonate or HEPES — how the two buffering routes differ

Property Sodium bicarbonate route HEPES route
Configurations in this family DCP-TBSSB-R1X, DCP-TBSSBR1X DCP-TBSSH1X
Buffer chemistry Inorganic bicarbonate, working as one half of a bicarbonate–CO2 pair[4] Zwitterionic Good's buffer, pKa near 7.3 at 37 °C[2]
Dependence on the gas phase Gas-coupled — the CO2 over the solution is part of the buffer system[4] Independent of CO2 concentration[2]
Effective buffering range Set by the bicarbonate–CO2 equilibrium Approximately pH 6.8–8.2[3]
Suits work outside an incubator cancel Not the intended condition check_circle Yes — extended manipulation in ambient air[2]
Stated pH of the finished solution 7.4 7.4
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed — support@diagnocine.com.
FAQ

Questions this catalog gets asked

TBSS is a physiologically isotonic buffer solution used in cell culture and tissue preservation. It maintains structural and physiological integrity by providing essential nutrients in a stable environment with appropriate pH and osmotic balance. The source names three uses in particular: mammalian body perfusion, tissue cleaning, and maintaining normal physiological functions in isolated intestinal muscle experiments.
Four things, as stated: it maintains intra- and extracellular osmotic balance, provides essential inorganic ions, supplies energy through glucose, and offers buffering capacity to maintain physiological pH.
All three configurations are supplied at pH 7.4. Two of them name sodium bicarbonate as the buffering component and one names HEPES. A bicarbonate system holds pH as one half of a bicarbonate and CO2 pair, so the gas phase is part of the buffer; HEPES buffering capacity is independent of CO2 concentration.[2,4]
When the work happens in open ambient air rather than under a controlled CO2 atmosphere — a perfusion rig, an organ bath, a microscope stage, or any extended manipulation outside an incubator. HEPES is a zwitterionic Good's buffer whose capacity does not depend on CO2, with an effective range of roughly pH 6.8 to 8.2 that comfortably brackets the stated pH 7.4.[2,3] DCP-TBSSH1X is the HEPES configuration.
It adds a visual pH read-out: the dye reports acidification by colour. The trade-off is optical — phenol red absorbs in the visible range and can quench fluorescent signal, so phenol-red-free formulations exist for fluorescence and imaging work.[5] DCP-TBSSBR1X names phenol red as present; DCP-TBSSB-R1X names it as absent.
Each configuration is supplied as a 1X liquid in a 500 mL fill. Every one is filtered 0.1 µm twice and 0.04 µm twice in a sterile environment — a four-pass architecture whose last two passes are sub-0.1 µm polishing steps. Storage is at 4 °C.
Yes. Other concentrations, additions of chemicals, compounds, proteins or supplements, a different pH, and other modifications are available — please inquire at support@diagnocine.com.
Scientific References

Primary literature and cited sources

The source description carries no citation or reference list. The entries below are supporting literature for the Tyrode's formulation and for the HEPES buffer class, followed by the external technical sources footnoted on this page. Neither primary paper carries a publisher DOI, so none is shown — no DOI has been invented.

Supporting Literature

  1. Tyrode MV. The mode of action of some purgative salts. Archives Internationales de Pharmacodynamie et de Thérapie. 1910;20:205–223. — the paper in which the Tyrode's formulation was published.
  2. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. — the paper that introduced HEPES and the other zwitterionic “Good's” buffers.

Footnoted Technical Sources

  1. ScienceDirect Topics. Tyrode's Solution — overview of composition and use in physiological experiments, including heart perfusion and dye-loading protocols. sciencedirect.com
  2. R&D Systems / Bio-Techne. HEPES Buffer Solution (1 M) — zwitterionic buffer, pKa 7.3 at 37 °C, buffering capacity independent of CO2 concentration. rndsystems.com
  3. Boston BioProducts. HEPES — pKa 7.5 at 25 °C and an effective buffering range of pH 6.8 to 8.2. bostonbioproducts.com
  4. Thermo Fisher Scientific. Balanced Salt Solutions — how the sodium bicarbonate level of a balanced salt solution sets the CO2 environment it is written for. thermofisher.com
  5. Thermo Fisher Scientific. Product FAQ on phenol red and fluorescent read-outs, and phenol-red-free formulations. thermofisher.com
  6. Cold Spring Harbor Protocols. HEPES-buffered Tyrode's solution — published recipe adjusted to pH 7.4. cshprotocols.cshlp.org
Configuration selection support. For help matching a configuration to a specific protocol, or for documentation requests including the Certificate of Analysis, contact support@diagnocine.com. Ready to order? Back to the Tyrode's Balanced Salt Solution catalog.

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