Tris-based Buffers
Product#: Tris-based Buffers
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Tris-based Buffers
Tris-buffered saline (TBS) is an isotonic, non-toxic buffer used in various biochemical experiments to maintain a narrow, slightly alkaline pH range of 7-9. While the conjugate acid of Tris has a pKa of 8.07 at 25 °C, the solution pH shift is observed as temperature changes (0.03 units per degree Celsius). TBS buffer is commonly used as a laboratory reagent for washing, diluting, cell culture processing, Western blotting, ELISA and multiple assays and immunohistochemistry steps. Tris acts as a buffering agent, and NaCl regulates the solution's tonicity, allowing it to be isotonic or hypertonic based on the desired concentration.
Protease (-), DNAse (-), RNAse (-), Endonuclease (-)
All Reagents Filtered TWICE (2X) through 0.1 micron membrane filters. No mycoplasma.
| Tris Buffered Saline | |||
| Name | Cat. No. | Size | Link to Order |
| TBS, 1X (Tris Buffered Saline), Non-Sterile | DCP-TBS1X_500ml | 500 ml |  |
| DCP-TBS1X_1000ml | 1000 ml | ||
| DCP-TBS1X_10 Liter | 10 L | ||
| TBS, 10X (Tris Buffered Saline), Non-Sterile | DCP-TBS10X_500 ml | 500 ml | |
| DCP-TBS10X_1000 ml | 1000 ml | ||
| TBS, 20X (Tris Buffered Saline), Non-Sterile | DCP-TBS20X_500 ml | 500 ml | |
| DCP-TBS20X_1000 ml | 1000 ml | ||
| TBST (Tris-buffered saline, 0.1% Tween 20) | DCP-TBST0.1X_500ml | 500 ml | |
| DCP-TBST0.1X_1 L | 1000 ml | ||
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Description:
Tris-HCl buffer is widely used in molecular biology techniques like gel electrophoresis, nucleic acid work, protein extraction and purification. It is used for preparing SDS-PAGE gel solutions, electrophoresis running buffers, and cell culture media. It is a mild buffer that does not significantly interfere with or inhibit most biochemical processes and protein-protein interactions. Its buffering capacity prevents abrupt changes in pH during purification steps, which could denature or degrade proteins.
| Name | Cat. No. | Size | Link to Order |
| Tris-HCl Buffer, Sterile | DCP-THB1X_100 ml | 100 ml | |
| DCP-THB1X_500 ml | 500 ml | ||
| DCP-THB1X_1000 ml | 1000 ml |
