Potassium Acetate Buffer [1X]
Cat No.: DCP-PAB1X
Size: 100 mL & 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Potassium Acetate Buffer is used to precipitate and concentrate DNA and RNA from aqueous solutions and is often combined with ethanol or isopropanol precipitation. The presence of potassium acetate enhances the precipitation efficiency of the alcohol. The potassium ions help to neutralize the negative charges on the phosphate backbone of DNA which reduces the solubility of DNA in aqueous solutions and promotes precipitation. Additionally, it can precipitate and remove SDS and SDS-bound proteins from DNA solutions, allowing for the purification of DNA from protein contaminants. Potassium acetate is particularly useful for precipitating RNA for applications like cell-free translation because it avoids introducing sodium ions, which can interfere with some processes.
Advantages:
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It has the ability to create the optimal ionic conditions and neutralize the negative charges on nucleic acids which facilitates efficient precipitation.
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It helps with the removal of SDS-bound proteins from DNA solutions.
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Potassium acetate buffers have a buffering capacity in the pH range of approximately 5.5, making them useful for applications that require an acidic pH environment.
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Stability: Potassium acetate buffer demonstrates good stability, which is crucial for consistent experimental results.
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Non-toxic: The buffer lacks toxicity, making it safe for use in biological systems.
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Optimal pH range: With a pKa between 6.0 and 8.0, potassium acetate buffer operates in the optimal range for most biological reactions.
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Minimal interference: The buffer has minimal salt effects due to its ionic composition and minimal participation in biological reactions.
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Purity and quality: Manufactured using highly purified, analytical grade chemicals, potassium acetate buffers are extensively quality tested and free from enzymatic and hydrolytic activity.
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DNA precipitation: In molecular biology applications, potassium acetate is used for ethanol precipitation of DNA.
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Protein removal: Potassium acetate precipitates sodium dodecyl sulfate (SDS) and SDS-bound proteins, allowing their removal from DNA samples.
Composition:
| Ingredients |
Concentration |
| Potassium acetate |
2 M |
| Glacial acetic acid |
2 M |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 5.5
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
