Flamma® 774 Isothiocyanate
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 774 Isothiocyanate
Cat. No. List below
Description
Flamma® Fluors 774 Isothiocyanate is particularly suited for protein labeling, antibody conjugation, nucleic acid labeling, in vitro imaging, in vivo NIR imaging, fluorescence-based detection methods, and ligand labeling.
The isothiocyanate group reacts with primary amines to form stable thiourea linkages. The
optimal pH is above 9 (higher than NHS ester labeling, which typically occurs at pH 8.3). It targets primary amines on proteins, peptides, and amino-modified nucleic acids. It is generally slower than NHS ester reactions, allowing for more controlled labeling.
Flamma® Fluors 774 Isothiocyanate represents a powerful tool for researchers in life sciences, offering superior NIR fluorescence properties and stable conjugation for advanced imaging and detection applications. Its versatility, high sensitivity, and stability make it an excellent choice for a wide range of biological research and diagnostic applications, including challenging in vivo NIR imaging studies. The isothiocyanate reactive group provides an alternative to NHS esters, offering researchers more options for optimizing their labeling protocols based on specific experimental requirements.
What are the advantages?
1. Stability: Moderately reactive but quite stable in water and most organic solvents, offering extended shelf life and flexibility in handling.
2. NIR Fluorescence: Emits in the NIR region, allowing for deep tissue imaging with minimal autofluorescence background.
3. High Signal-to-Noise Ratio: Provides clear and distinct signals, crucial for sensitive detection applications.
4. Versatility: Compatible with various excitation sources, including 750 nm or 785 nm laser lines and dye-pumped lasers.
5. Stable Conjugation: Forms reasonably stable thiourea linkages with biomolecules, ensuring long-lasting labeling.
6. Wide Application Range: Suitable for labeling various biomolecules, including proteins, antibodies, peptides, and nucleic acids.
- Fluorophore: Flamma® Fluors 774
- Reactive group: Isothiocyanate
- Excitation/Emission Max.(nm): 774/800
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800
- Extinction coefficient: ≥ 182,000 cm-1M-1
- CF280: 0.1
- Appearance: Green Solid
- Molecular Weight: 1013 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWI1001 | Flamma® 496 Isothiocyanate | 494 | 520 | Alexa488, FITC, Cy2 |
| PWI1122 | Flamma® 552 Isothiocyanate | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWI1415 | Flamma® 581 Isothiocyanate | 578 | 593 | Alexa594, DyLight594 |
| KWI1215 | Flamma® 648 Isothiocyanate | 648 | 663 | Alexa647, DyLight650, Cy5 |
| KWI1515 | Flamma® 675 Isothiocyanate | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWI1308 | Flamma® 749 Isothiocyanate | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWI1603 | Flamma® 774 Isothiocyanate | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Isothiocyanate
BioActs offers a series of Flamma® Fluors isothiocyanates, which are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates form reasonably stable thiourea linkage upon reaction with amines. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. This basic isothiocyanate labeling condition may be a factor for working with basic-environment tolerable biomolecules that DNA and most polysaccharides can be modified in a relatively basic pH.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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