Flamma® 648 Isothiocyanate
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 648 Isothiocyanate
Cat. No. List below
Description
Key Features
1. Excitation/Emission maxima at 648/663 nm
2. Compatible with various laser lines in the far-red spectrum
3. High extinction coefficient of ≥ 227,000 cm?¹M?¹
4. Excellent optical properties and brightness
5. Excellent chemical stability in water and most organic solvents
6. Low CF280 value of 0.03, minimizing interference with protein measurements
7. Optimal conjugation at pH above 9
Applications
1. Fluorescence microscopy
2. Flow cytometry
3. In vivo imaging
4. Protein and peptide labeling
5. Immunofluorescence
6. Long-term cellular tracking studies
7. Labeling of antibodies, ligands, and amplification substrates
8. Amino-modified oligonucleotide labeling
Advantages
1. Exceptional brightness and photostability for clear, long-lasting signals
2. Versatile conjugation to various biomolecules
3. Ideal for long-term studies and challenging experimental conditions
4. Reduced autofluorescence and improved tissue penetration due to far-red spectrum
5. Spectral similarity to popular dyes (Alexa647, DyLight650, Cy5) for easy protocol integration
6. Suitable for deep-tissue and whole-body imaging applications
7. Enhanced stability compared to NHS esters in aqueous solutions
Flamma® Fluors 648 Isothiocyanate is part of the broader Flamma® Fluors series, which covers a full spectral range from UV to NIR. These dyes are characterized by strong absorption, high fluorescence quantum yield, and excellent photostability. They maintain good fluorescence activity and stability after conjugation to biomolecules, allowing for the detection of low-abundance biological structures with great sensitivity.
The isothiocyanate reactive group offers unique advantages for protein modification, particularly at pH levels above 9. This makes it especially suitable for labeling basic-environment tolerant biomolecules such as DNA and most polysaccharides, as well as proteins that maintain stability at higher pH levels.
Researchers can leverage Flamma® Fluors 648 Isothiocyanate for a wide range of applications in life sciences and biotechnology. Its superior optical properties, stability, and versatility make it a valuable tool for cutting-edge biological studies, drug discovery, and medical diagnostics. The dye's far-red spectral characteristics make it particularly useful for deep-tissue imaging and in vivo applications, where reduced light scattering and absorption by biological tissues are crucial.
- Fluorophore: Flamma® Fluors 648
- Reactive group: Isothiocyanate
- Excitation/Emission Max.(nm): 648/663
- Spectrally similar dyes: Alexa647, DyLight650, Cy5
- Extinction coefficient: ≥ 227,000 cm-1M-1
- CF280: 0.03
- Appearance: Blue Solid
- Molecular Weight: 769.01 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWI1001 | Flamma® 496 Isothiocyanate | 494 | 520 | Alexa488, FITC, Cy2 |
| PWI1122 | Flamma® 552 Isothiocyanate | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWI1415 | Flamma® 581 Isothiocyanate | 578 | 593 | Alexa594, DyLight594 |
| KWI1215 | Flamma® 648 Isothiocyanate | 648 | 663 | Alexa647, DyLight650, Cy5 |
| KWI1515 | Flamma® 675 Isothiocyanate | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWI1308 | Flamma® 749 Isothiocyanate | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWI1603 | Flamma® 774 Isothiocyanate | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Stability: Excellent stability in water and organic solvents
- Versatility: Suitable for a wide range of pH conditions
- Brightness: High extinction coefficient for enhanced sensitivity
- Durability: Forms stable thiourea linkages for long-term studies
| Feature | Flamma® Fluors 648 Isothiocyanate | NHS Ester Dyes |
| Optimal pH for Labeling | Above 9 | Around 8.3 |
| Stability in Aqueous Solutions | High | Moderate |
| Linkage Type | Thiourea | Amide |
| Labeling Stability | Excellent | Good |
| Versatility in Solvents | High | Moderate |
Flamma® Fluors Isothiocyanate
BioActs offers a series of Flamma® Fluors isothiocyanates, which are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates form reasonably stable thiourea linkage upon reaction with amines. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. This basic isothiocyanate labeling condition may be a factor for working with basic-environment tolerable biomolecules that DNA and most polysaccharides can be modified in a relatively basic pH.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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