Flamma® 749 Thiol
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 Thiol
Cat. No. List below
Description
Flamma® Fluors 749 Thiol is an advanced near-infrared (NIR) fluorescent dye derived from cyanine structure, specifically designed for generating stable fluorescence signals in bioimaging applications. This inactive form of the dye features a thiol group attached to the Flamma 749 fluorophore core through a spacer, offering unique labeling possibilities.
The dye exhibits excitation and emission maxima at 749 nm and 774 nm, respectively, positioning it firmly in the NIR region of the spectrum. These spectral characteristics are comparable to other popular NIR dyes such as Alexa 750, Cy7, IRDye 750, and DyLight 755. Flamma 749 can be efficiently excited using a 750 nm laser line or dye-pumped laser, with emission occurring in the NIR region. This spectral range is particularly advantageous for biological tissue imaging due to improved penetration and reduced autofluorescence.
Flamma® Fluors 749 Thiol is particularly useful for bioimaging (generates stable fluorescence signals in various biological imaging applications), protein labeling (can form disulfide bonds with thiol groups of cysteine residues in proteins), and reference standard: Serves as a reference standard for dye-conjugates.
The thiol functional group of Flamma® Fluors 749 Thiol allows for specific labeling of biomolecules through disulfide bond formation with cysteine residues. This labeling strategy offers several advantages such as site-specific labeling of proteins, reversible labeling under reducing conditions and potential for controlled release applications.
Flamma® Fluors 749 Thiol advantages:
1. Reduced photobleaching compared to visible light fluorophores
2. Lower background fluorescence in biological samples
3. Potential for multiplexing with other fluorophores in different spectral regions
4. Enhanced tissue penetration for in vivo imaging applications
The thiol functionality of this dye also opens up possibilities for click chemistry applications, conjugation to nanoparticles or other thiol-reactive surfaces, and development of activatable probes based on disulfide chemistry.
These properties make Flamma® Fluors 749 Thiol a valuable tool for researchers in fields such as molecular biology, biochemistry, and biomedical imaging, where high-sensitivity detection, specific labeling, and low background interference are crucial. Its unique thiol reactivity expands the toolkit available for bioconjugation and probe development in the NIR spectral range.
- Fluorophore: Flamma® Fluors 749
- Functional group: Thiol
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 150,000 cm-1M-1
- CF280: 0.103
- Appearance: Green Solid
- Molecular Weight: 772.05 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWT1001 | Flamma® 496 Thiol | 494 | 520 | Alexa488, FITC, Cy2 |
| CWT1058 | Flamma® 552 Thiol | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWT1415 | Flamma® 581 Thiol | 578 | 593 | Alexa594, DyLight594 |
| KWT1042 | Flamma® 648 Thiol | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWT1415 | Flamma® 675 Thiol | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWT1215 | Flamma® 749 Thiol | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWT1515 | Flamma® 774 Thiol | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Thiol
Flamma® Fluors thiol dyes have an attached primary thiol, which connected through a spacer. These thiol dyes can be labeled to biomolecules through disulfide bond formation with thiol of cysteine residue.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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