Flamma® 675 ADIBO
Size of product (mg)
-
1 mg
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5 mg
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25 mg
Flamma® 675 ADIBO
Cat. No. List below
Description
Spectral Properties:
Excitation/Emission maxima: 674/688 nm
Spectrally similar to Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680
Can be excited using a 633 nm laser line
Emits in the NIR region, allowing for deep tissue penetration
Chemical Reactivity
Flamma 675 ADIBO is engineered for copper-free click chemistry reactions. It couples with azide-functionalized biomolecules to form 1,4-disubstituted 1,2,3-triazoles through SPAAC reactions. This process occurs efficiently in living systems without the need for coupling reagents or catalysts, preserving native biochemical processes.
Its applications are cellular imaging, nucleotide functionalization, in vivo and in vitro bioimaging, and bioorthogonal labeling in complex biological environments.
To utilize Flamma 675 ADIBO, introduce azide functionality to the target biomolecule through chemical or genetic modification. Then perform the SPAAC reaction to couple the dye with the azide-modified target. Finally, proceed with imaging or further experimental procedures
The NIR emission of Flamma 675 ADIBO makes it particularly valuable for in vivo imaging applications. NIR light can penetrate deeper into biological tissues compared to visible light, allowing for enhanced signal-to-noise ratios and improved imaging depth.
The ADIBO reactive group's strain-promoted cycloaddition chemistry allows for rapid and efficient labeling under mild conditions, It is ideal for sensitive biological samples and live-cell imaging
Potential for multiplexing with other fluorophores due to its distinct spectral properties
Flamma® Fluors 675 ADIBO represents a state-of-the-art tool in modern biomedical research, offering high sensitivity, specificity, and versatility for a wide range of imaging and labeling applications, particularly where copper-free click chemistry is desired or required.
- Fluorophore: Flamma® Fluors 675
- Reactive group: ADIBO
- Excitation/Emission Max.(nm): 674/688
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT
- Extinction coefficient: ≥ 220,000 cm-1M-1
- Appearance: Blue Solid
- Molecular Weight: 1203.42 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| DWC1001 | Flamma® 496 ADIBO | 496 | 520 | Alexa488, FITC, Cy2 |
| DWC1011 | Flamma® 552 ADIBO | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| DWC1415 | Flamma® 581 ADIBO | 581 | 596 | Alexa594, DyLight594 |
| DWC1021 | Flamma® 648 ADIBO | 648 | 663 | Alexa647, DyLight650, Cy5 |
| DWC1051 | Flamma® 675 ADIBO | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| DWC1031 | Flamma® 749 ADIBO | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| DWC1061 | Flamma® 774 ADIBO | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Copper-free: Eliminates potential toxicity associated with copper catalysts
- Fast reaction kinetics: ADIBO's strained alkyne structure promotes rapid cycloaddition
- Biocompatible: Efficient under physiological conditions
- Clean reaction: Produces no interfering by-products
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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