Flamma® 496 Hydrazide
Size of product (mg)
-
1 mg
-
5 mg
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25 mg
Flamma® 496 Hydrazide
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 496/520 nm
2. High extinction coefficient: ≥ 43,000 cm?¹M?¹
3. Hydrazide reactive group for specific aldehyde and ketone labeling
4. Yellow solid appearance
5. Molecular weight: 428.34 g/mol
6. Soluble in DMF and DMSO
7. Spectrally similar to Alexa488, FITC, and Cy2
Applications:
1. Labeling of polysaccharides and glycoproteins
2. Visualization of free reducing sugars on biomolecules
3. Bioimaging of aldehyde and ketone-bearing molecules
4. Potential use in flow cytometry and fluorescence microscopy
5. Applicable in studies involving carbohydrate chemistry and glycobiology
Advantages:
1. Generates stable fluorescence signals in bioimaging
2. Excellent optical properties when excited with a 488 nm laser line
3. Forms stable imine linkages through reductive amination reactions
4. Enables labeling of biomolecules after oxidation of primary and secondary alcohols
5. Offers compatibility with common fluorescence instrumentation
6. Provides a bright green fluorescence signal with minimal background
Flamma® Fluors 496 Hydrazide exhibits maximal excitation at 496 nm and emission at 520 nm, placing it in the same spectral range as other popular green fluorescent dyes. This spectral similarity allows for easy integration into existing imaging protocols and systems using standard FITC filter sets.
The hydrazide reactive group of Flamma® 496 is highly selective for aldehyde and ketone groups, forming stable imine linkages through reductive amination reactions. This selectivity makes it particularly useful for labeling free reducing sugars on biomolecules. For applications involving polysaccharides and glycoproteins, researchers can oxidize primary and secondary alcohols to aldehydes and ketones prior to conjugation, expanding the range of targetable molecules.
Engineered to perform in complex biological environments, Flamma® Fluors 496 Hydrazide exhibits excellent solubility in DMF and DMSO. Its bright green fluorescence and high extinction coefficient ensure strong signal generation even at low concentrations, enabling the visualization of low-abundance biological structures with great sensitivity.
The dye's stability and excellent optical properties make it suitable for various advanced bioimaging techniques, including fluorescence microscopy and flow cytometry. Its compatibility with the 488 nm laser line, commonly found in many imaging systems, further enhances its versatility in research settings.
For optimal results and longevity, store Flamma® Fluors 496 Hydrazide at -20°C and protect it from light. With its superior brightness, excellent photostability, and specific reactivity towards aldehydes and ketones, this dye empowers researchers to achieve high-quality imaging results in studies involving carbohydrate chemistry, glycobiology, and related fields.
- Fluorophore: Flamma® Fluors 496
- Reactive group: Hydrazide
- Excitation/Emission Max.(nm): 496/520
- Spectrally similar dyes: Alexa488, FITC, Cy2
- Extinction coefficient: ≥ 43,000 cm-1M-1
- CF280: 0.12
- Appearance: Yellow Solid
- Molecular Weight: 428.34 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWH1001 | Flamma® 496 Hydrazide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWH1122 | Flamma® 552 Hydrazide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWH1415 | Flamma® 581 Hydrazide | 578 | 593 | Alexa594, DyLight594 |
| PWH1215 | Flamma® 648 Hydrazide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWH1515 | Flamma® 675 Hydrazide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWH1301 | Flamma® 749 Hydrazide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWH1603 | Flamma® 774 Hydrazide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Hydrazide
Flamma® Fluors hydrazide dyes can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. Fluorescent modification of aldehyde or carbonyl groups in carbohydrates is also frequently utilized for their analysis by HPLC, capillary electrophoresis and other methods. Hydrazide dyes can also label biomolecules, which introduced aldehyde by genetical or chemical modification.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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