Flamma® 581 Isothiocyanate
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 581 Isothiocyanate
Cat. No. List below
Description
Key Features
1. Excitation/Emission maxima at 578/593 nm
2. Compatible with 561, 568, or 578 nm laser lines
3. High extinction coefficient of ≥ 109,000 cm?¹M?¹
4. Excellent optical properties and brightness
5. Moderate reactivity with high stability in water and most organic solvents
6. Forms stable thiourea linkages with primary amines
7. Optimal conjugation at pH above 9
Applications
1. Antibody labeling
2. Peptide and protein tagging
3. Ligand labeling
4. Amplification substrate optimization
5. Cellular labeling and detection
6. Fluorescence microscopy
7. Flow cytometry
8. In vivo imaging
9. Single-molecule detection
10. High-content screening
Advantages
1. Spectral similarity to popular dyes (Alexa594, DyLight594) allows easy integration into existing protocols
2. Superior brightness and photostability compared to traditional fluorophores
3. Versatile conjugation to various biomolecules
4. Stable in challenging experimental conditions
5. Low CF280 value (0.18) minimizes interference with protein absorbance measurements
6. Excellent solubility in DMF and DMSO for flexible experimental design
7. More stable than NHS esters, leading to longer shelf life
8. Suitable for labeling basic-environment tolerable biomolecules
Flamma® Fluors 581 Isothiocyanate is part of the broader Flamma® Fluors series, which covers a full spectral range from UV to NIR. These dyes are characterized by strong absorption, high fluorescence quantum yield, and excellent photostability. They maintain good fluorescence activity and stability after conjugation to biomolecules, allowing for the detection of low-abundance biological structures with great sensitivity.
The isothiocyanate reactive group offers unique advantages for protein modification. While NHS esters typically require a pH of 8.3 for protein labeling, Flamma® 581 Isothiocyanate performs optimally at pH above 9. This higher pH requirement makes it particularly suitable for labeling basic-environment tolerant biomolecules such as DNA and most polysaccharides.
Researchers can leverage Flamma® 581 Isothiocyanate for a wide range of applications in life sciences and biotechnology. Its superior optical properties, stability, and versatility make it a valuable tool for cutting-edge biological studies, drug discovery, and medical diagnostics. The dye's compatibility with various laser lines and fluorescence imaging systems ensures its utility across different experimental setups and instrumentation.
- Fluorophore: Flamma® Fluors 581
- Reactive group: Isothiocyanate
- Excitation/Emission Max.(nm): 578/593
- Spectrally similar dyes: Alexa594, DyLight594
- Extinction coefficient: ≥ 109,000 cm-1M-1
- CF280: 0.18
- Appearance: Purple Solid
- Molecular Weight: 1003.21 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWI1001 | Flamma® 496 Isothiocyanate | 494 | 520 | Alexa488, FITC, Cy2 |
| PWI1122 | Flamma® 552 Isothiocyanate | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWI1415 | Flamma® 581 Isothiocyanate | 578 | 593 | Alexa594, DyLight594 |
| KWI1215 | Flamma® 648 Isothiocyanate | 648 | 663 | Alexa647, DyLight650, Cy5 |
| KWI1515 | Flamma® 675 Isothiocyanate | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWI1308 | Flamma® 749 Isothiocyanate | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWI1603 | Flamma® 774 Isothiocyanate | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Isothiocyanate
BioActs offers a series of Flamma® Fluors isothiocyanates, which are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates form reasonably stable thiourea linkage upon reaction with amines. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. This basic isothiocyanate labeling condition may be a factor for working with basic-environment tolerable biomolecules that DNA and most polysaccharides can be modified in a relatively basic pH.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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