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Flamma® 581 Maleimide

Product#: KWM1415

$1,100.80

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Flamma® 581 Maleimide

Cat. No. List below

Description

Flamma® Fluors 581 Maleimide is a versatile thiol-reactive fluorescent dye designed for generating stable fluorescence signals in bioimaging applications. This bright orange dye exhibits maximal excitation at 581 nm and emission at 596 nm, making it spectrally similar to Alexa 594, DyLight 594, and a suitable replacement for Cy3.5 and Alexa 568.

Key features:
1. Excitation/Emission maxima: 581/596 nm
2. High extinction coefficient: ≥ 128,000 M-1 cm-1
3. Excellent photostability and brightness
4. Maleimide reactive group for specific thiol labeling
5. Purple solid appearance
6. Molecular weight: 1041.19 g/mol
7. Soluble in DMF and DMSO

Applications:
1. Labeling of thiols on antibodies, peptides, proteins, and ligands
2. Cellular labeling and detection
3. Fluorescence microscopy and imaging
4. Amplification substrate labeling
5. Bioconjugation techniques

Advantages:
1. Selective labeling of cysteine residues via 1,4-addition pathway
2. High sensitivity for detecting low-abundance biomolecules
3. Compatible with 561, 568, or 578 nm laser excitation
4. Minimal reactivity with other amino acids (methionine, histidine, tyrosine)
5. Stable thioether linkage formation
6. Excellent optical properties for various fluorescence applications

Flamma® 581 Maleimide offers researchers a powerful tool for precise and sensitive labeling of thiol-containing biomolecules. Its specific reactivity towards cysteine residues, combined with its bright fluorescence and stability, makes it an ideal choice for a wide range of biological studies and imaging applications. The dye's spectral characteristics allow for multicolor imaging experiments and its high extinction coefficient ensures strong signal generation even at low concentrations.

For optimal results, store Flamma® 581 Maleimide at 4°C and protect it from light. Researchers can confidently use this dye for various applications, including protein labeling, cellular imaging, and fluorescence-based assays, knowing that it provides reliable and sensitive detection of their target molecules.

Specifications

Fluorophore: Flamma® Fluors 581
Reactive group: Maleimide
Excitation/Emission Max.(nm): 581/596
Spectrally similar dyes: Alexa594, DyLight594
Extinction coefficient: ≥ 109,000 cm-1M-1
CF280: 0.18
Appearance: Purple Solid
Molecular Weight: 1041.19 g/mol
Solubility: DMF, DMSO
Storage conditions: 4 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWM1001	Flamma® 496 Maleimide	496	520	Alexa488, FAM
CWM1058	Flamma® 552 Maleimide	550	565	Alexa555, DyLight549, Cy3, ATTO550, CF555
KWM1415	Flamma® 581 Maleimide	581	596	Alexa594, DyLight594
KWM1042	Flamma® 648 Maleimide	648	663	Alexa647, DyLight650, Cy5, ATTO 647N, CF647
PWM1415	Flamma® 675 Maleimide	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWM1215	Flamma® 749 Maleimide	749	774	Alexa750, DyLight755, Cy7, IRDye750
PWM1515	Flamma® 774 Maleimide	774	806	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Flamma^® Fluors maleimide

Maleimide is an excellent reactive group that can specially label thiol of cysteine residue without interacting with amino functionality. In labeling process, thiol is added to the double bond of maleimide via 1,4-addition pathway to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they are reacted with amines in higher pH than reaction of maleimides. BioActs offers Flamma^® Fluors maleimide series as thiol-reactive fluorescence dyes.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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