/Content/Upload/Product/Flamma648Thiol_1200.jpg

Flamma® 648 Thiol

Product#: KWT1042

$4,143.84

Size of product (mg)

1 mg
5 mg
25 mg

Qty:

Availability:

Ships in 1-2 Weeks

Flamma® 648 Thiol

Cat. No. List below

Description

Flamma® Fluors 648 Thiol is an advanced far-red fluorescent dye designed for cutting-edge bioimaging applications. Derived from a cyanine structure, this versatile fluorophore offers exceptional stability and optical properties for generating reliable fluorescence signals in complex biological environments.

Key features:
1. Excitation/Emission maxima: 648/663 nm
2. High extinction coefficient: ≥ 250,000 cm?¹M?¹
3. CF280: 0.03
4. Thiol functional group attached through a spacer
5. Blue solid appearance
6. Molecular weight: 743.99 g/mol
7. Soluble in DMF and DMSO

Applications:
1. Bioimaging and fluorescence microscopy
2. Labeling of biomolecules through disulfide bond formation
3. Reference standard for dye-conjugates
4. Super-resolution microscopy
5. Single-molecule detection
6. Fluorescence in situ hybridization (FISH)
7. Flow cytometry
8. Confocal laser scanning microscopy
9. Live-cell imaging
10. Deep-tissue and whole-body imaging

Advantages:
1. Operates in the optimal far-red spectrum, minimizing autofluorescence
2. Excellent signal-to-noise ratios
3. Spectral similarity to popular dyes (Alexa647, DyLight650, Cy5)
4. Efficient excitation using 593 or 633 nm laser lines
5. Versatile labeling options through disulfide bond formation with cysteine residues
6. Stable fluorescence signal for extended imaging sessions
7. High sensitivity for detecting low-abundance biological structures
8. Enhanced tissue penetration and reduced light scattering
9. Reduced phototoxicity to biological components
10. Compatibility with the "biological window" (650-900 nm) for improved in vivo imaging

Flamma® Fluors 648 Thiol features a unique structure where the thiol group is attached to the fluorophore through a spacer, enhancing its reactivity and flexibility in various experimental conditions. The dye's far-red spectral properties position it within the optimal "biological window," where light absorption by tissues is minimal, allowing for deeper penetration and improved signal-to-noise ratios in complex biological samples.

Specifications

Fluorophore: Flamma® Fluors 648
Functional group: Thiol
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 250,000 cm-1M-1
CF280: 0.03
Appearance: Blue Solid
Molecular Weight: 743.99 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWT1001	Flamma® 496 Thiol	494	520	Alexa488, FITC, Cy2
CWT1058	Flamma® 552 Thiol	550	564	Alexa555, DyLight549, Cy3, ATTO550
KWT1415	Flamma® 581 Thiol	578	593	Alexa594, DyLight594
KWT1042	Flamma® 648 Thiol	648	663	Alexa647, DyLight650, Cy5
PWT1415	Flamma® 675 Thiol	674	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWT1215	Flamma® 749 Thiol	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWT1515	Flamma® 774 Thiol	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Flamma^® Fluors Thiol

Flamma^® Fluors thiol dyes have an attached primary thiol, which connected through a spacer. These thiol dyes can be labeled to biomolecules through disulfide bond formation with thiol of cysteine residue.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.