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qFlamma® Black01 Azide

Product#: QWZ1001

$778.24

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5 mg
25 mg

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qFlamma® Black01 Azide

Cat. No. List below

Description

qFlamma® Black01 Azide is a versatile click chemistry reagent designed as a universal dark quencher for various biochemical and biological research applications. This green solid compound exhibits exceptional properties, including a high absorption coefficient, remarkable stability, and excellent aqueous solubility. The Black01 quencher component demonstrates a broad absorption range from 400 to 800 nm, with a maximum absorption at 490 nm, and functions without native fluorescence13.

The azide moiety of qFlamma® Black01 Azide enables its use in both copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC) reactions. In these reactions, the azide couples with an alkyne to form a 1,4-disubstituted 1,2,3-triazole1. This versatility allows researchers to introduce the quencher to various probes or biomolecules through chemical or genetic modification.

qFlamma® Black01 Azide is soluble in water, DMF, and DMSO, making it compatible with a wide range of experimental conditions. For optimal performance and longevity, it should be stored at -20°C and protected from light

Applications
1. Design of fluorescence resonance energy transfer (FRET) probes
2. Development of activatable probes for various biological assays
3. Nucleic acid hybridization studies
4. Multiplexed bio-analysis
5. Click chemistry-based labeling of biomolecules
6. Protein and peptide modification
7. Cellular imaging and microscopy techniques

Advantages
1. Broad absorption range: Effectively quenches a wide variety of commercially available fluorescent dyes from 400 to 800 nm
2. High absorption coefficient: Ensures efficient quenching of fluorescence
3. Superb stability: Maintains performance under various experimental conditions
4. Excellent aqueous solubility: Facilitates easy manipulation in aqueous buffers
5. No native fluorescence: Eliminates background fluorescence, improving signal-to-noise ratio
6. Versatility in click chemistry reactions: Compatible with both CuAAC and SPAAC, offering flexibility in experimental design
7. Compatibility: Works well with most biomolecules and can be easily introduced through chemical or genetic modification
8. Efficient quenching: Provides superior quenching efficiency compared to traditional quenchers like Dabcyl, especially for far-red dyes

These features make qFlamma® Black01 Azide an excellent choice for researchers seeking a reliable and efficient dark quencher for their experiments in biochemical and biological fields, particularly those involving click chemistry applications.

Specifications

Functionality: Dark quencher
Reactive group: azide
Absorption max. (nm): 490
Absorption range (nm): 400 ~ 800
Appearance: green solid
Solubility: Water, DMF, DMSO
Storage conditions: -20 ℃, protect from light

qFlamma® Quenchers

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
QWS1001	qFlamma® Black01 NHS ester	QWSN1001	qFlamma® Black01 Sulfo-NHS ester
QWA1001	qFlamma® Black01 Vinylsulfone	QWC1001	qFlamma® Black01 Carboxylic acid
QWM1001	qFlamma® Black01 Maleimide	QWE1001	qFlamma® Black01 Amine
QWT1001	qFlamma® Black01 Thiol	QWR1001	qFlamma® Black01 Dichlorotriazine
QWG1001	qFlamma® Black01 PEG4-Alkyne	QWD1001	qFlamma® Black01 ADIBO
QWB1001	qFlamma® Black01 Biotin	QWK1001	qFlamma® Black01 Alkyne
QWZ1001	qFlamma® Black01 Azide	QWH1001	qFlamma® Black01 Hydrazide

Background

qFlamma® Quenchers

Dark quenchers are substances that absorb excitation energy from a fluorophore and dissipate the energy as heat without any native fluorescence. The combination of a fluorescent dye and quencher may act as an on/off switch of fluorescence that the original fluorescence might be restored as the distance between them increases or when their connection is cleaved. In conjunction with fluorophores, dark quenchers are regarded as key components in designing FRET probes, in protease activity assays, nucleic acid hybridization, PCR, etc. The main advantage of dark quencher is the non-emissive excitation and elimination of background fluorescence. BioActs developed a new generation of azo-anthraquinone derivative dark quenchers, qFlamma® quenchers, to overcome issues associated with existing dark quenchers such as stability, quenching ability and water solubility.

The absorbance peaks of qFlamma® quenchers completely cover spectral range from visible to near-infrared (NIR) that all of commercially available fluorescent dyes can be effectively quenched. The quenchers display high absorption coefficient together with excellent quenching ability, superb stability and aqueous solubility so that it can easily manipulated in aqueous buffers. The four of qFlamma® quenchers (Black 01, Red, Blue and Orange) series cover the electromagnetic spectrum from 400 nm to NIR region and provides excellent spectral overlap across the entire range of known fluorescent dyes. The complete spectral overlap of qFlamma® quencher series along with their variety of reacting groups enable to design single-tube multiplexing assay system owing their capability for efficient quenching and the preventing cross-talking between dyes. BioActs offers qFlamma® quenchers as universal dark quenchers for the construction of a variety of hybridization and custom-synthesized probes incorporated spectrally paired fluorophores and quenchers for multiplexed bio-analysis, PCR, nucleic acid hybridization, and high-throughput imaging assays.

Completely dissipate fluorescent energy without native fluorescence
Can quench all of commercially available fluorescent dyes
High absorption coefficient, excellent quenching ability, superb stability and aqueous solubility
High purity and compatible with most of biomolecules.

Types of qFlamma^® Quenchers

Figure 1. Absorption spectra of 4 qFlamma^®quenchers with emission maxima of commonly used fluorescent dyes.

Citation & Reference

1. Arnaud Chevalier. Bioconjugatable Azo-Based Dark-Quencher Dyes: Synthesis and Application to Protease-Activatable Far-Red Fluorescent Probes. Chemistry 19.5 (2013): 1686-99.

2. Arnaud Chevalier. Universal Dark Quencher Based on “Clicked” Spectrally Distinct Azo Dyes. Org. Lett 2013 15.23 (2013): 6082–6085.

3. Arnaud Chevalier. Straightforward synthesis of bioconjugatable azo dyes. Part 1: Black Hole Quencher-1 (BHQ-1) scaffold. Tetrahedron Letters 55.50 (2014): 6759-6763.