Flamma® 749 Isothiocyanate
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 Isothiocyanate
Cat. No. List below
Description
Flamma® Fluors 749 Isothiocyanate is an advanced near-infrared (NIR) fluorescent dye designed for high-performance bioimaging applications. This reactive dye, derived from a cyanine structure, offers a stable and intense fluorescence signal ideal for various biological imaging techniques.
Flamma 749 Isothiocyanate is particularly useful for antibody labeling (primary and secondary), protein and peptide conjugation, in vitro imaging, in vivo NIR imaging, and fluorescence-based detection methods.
To utilize Flamma 749 Isothiocyanate effectively, first prepare the biomolecule (protein, antibody, or peptide) in a buffer with pH above 9. Then react the isothiocyanate group with primary amines under alkaline conditions. Finally purify the conjugate to remove unreacted dye. Use the labeled molecule for imaging or other fluorescence-based applications.
The NIR emission of Flamma 749 makes it particularly valuable for in vivo and deep tissue imaging applications. Its spectral properties allow for multiplexing with other fluorophores in multi-color imaging experiments.
The high extinction coefficient and low CF280 value (0.03) indicate that Flamma 749 can provide strong fluorescence signals with minimal interference in protein quantification assays based on absorption at 280 nm.
The isothiocyanate reactive group offers an alternative to NHS esters for labeling, particularly when working with pH-sensitive biomolecules or when longer reaction times are preferred. The stability of isothiocyanates in various solvents also provides flexibility in experimental design.
Flamma® Fluors 749 Isothiocyanate represents a powerful tool in modern biomedical research, offering high sensitivity, specificity, and versatility for a wide range of imaging and labeling applications, particularly where NIR fluorescence is desired for deep tissue or in vivo imaging under alkaline conditions.
What are the advantages?
Stability: Moderately reactive but stable in various solvents
High Brightness: With an extinction coefficient of ≥ 200,000 cm?¹M?¹, Flamma 749 offers exceptional brightness for sensitive detection
NIR Emission: Ideal for deep tissue imaging with minimal autofluorescence
Versatile Conjugation: Forms stable thiourea linkages with primary amines
pH Flexibility: Can be used in alkaline conditions (pH > 9) for optimal labeling
- Fluorophore: Flamma® Fluors 749
- Reactive group: Isothiocyanate
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.03
- Appearance: Green Solid
- Molecular Weight: 795 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWI1001 | Flamma® 496 Isothiocyanate | 494 | 520 | Alexa488, FITC, Cy2 |
| PWI1122 | Flamma® 552 Isothiocyanate | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWI1415 | Flamma® 581 Isothiocyanate | 578 | 593 | Alexa594, DyLight594 |
| KWI1215 | Flamma® 648 Isothiocyanate | 648 | 663 | Alexa647, DyLight650, Cy5 |
| KWI1515 | Flamma® 675 Isothiocyanate | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWI1308 | Flamma® 749 Isothiocyanate | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWI1603 | Flamma® 774 Isothiocyanate | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Isothiocyanate
BioActs offers a series of Flamma® Fluors isothiocyanates, which are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates form reasonably stable thiourea linkage upon reaction with amines. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. This basic isothiocyanate labeling condition may be a factor for working with basic-environment tolerable biomolecules that DNA and most polysaccharides can be modified in a relatively basic pH.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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