Flamma® 749 ADIBO
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 ADIBO
Cat. No. List below
Description
The dye exhibits excitation and emission maxima at 749 nm and 774 nm, respectively, which places it in the NIR region of the spectrum. These spectral characteristics are similar to other well-known NIR dyes such as Alexa 750, Cy7, IRDye 750, and DyLight 755. Flamma 749 can be efficiently excited using a 750 nm laser line or dye-pumped laser, with emission occurring in the NIR region, making it particularly advantageous for biological tissue imaging due to improved penetration and reduced autofluorescence.
Flamma® Fluors 749 ADIBO participates in SPAAC reactions, coupling with azide-functionalized biomolecules to form 1,4-disubstituted 1,2,3-triazoles without the need for coupling reagents or catalysts. This unique feature allows for efficient labeling inside living systems while preserving native biochemical processes. To utilize this dye effectively, researchers must first introduce azide functionality onto the target biomolecule through chemical or genetic modification.
Flamma® Fluors 749 ADIBO is particularly useful for cellular imaging (provides stable fluorescence signals for various biological imaging applications), nucleotide functionalization (enables specific labeling of nucleic acids), bioconjugation (facilitates the attachment of the dye to various biomolecules through click chemistry), and in vivo imaging (the NIR properties make it suitable for deep tissue imaging without interfering with biological systems).
Flamma® Fluors 749 ADIBO advantages:
1. Reduced Background Fluorescence: The NIR properties minimize interference from biological samples.
2. Enhanced Tissue Penetration: Suitable for in vivo applications where deep tissue imaging is required.
3. Versatile Labeling Options: Can be used for a broad range of biomolecules once azide groups are introduced.
These features make Flamma® Fluors 749 ADIBO a valuable tool for researchers in molecular biology, biochemistry, and biomedical imaging. Its unique reactivity and spectral properties expand the toolkit available for bioconjugation and probe development, particularly in studying complex biological systems within living organisms.
- Fluorophore: Flamma® Fluors 749
- Reactive group: ADIBO
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 145,000 cm-1M-1
- Appearance: Green Solid
- Molecular Weight: 969 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| DWC1001 | Flamma® 496 ADIBO | 496 | 520 | Alexa488, FITC, Cy2 |
| DWC1011 | Flamma® 552 ADIBO | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| DWC1415 | Flamma® 581 ADIBO | 581 | 596 | Alexa594, DyLight594 |
| DWC1021 | Flamma® 648 ADIBO | 648 | 663 | Alexa647, DyLight650, Cy5 |
| DWC1051 | Flamma® 675 ADIBO | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| DWC1031 | Flamma® 749 ADIBO | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| DWC1061 | Flamma® 774 ADIBO | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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