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ICG Sulfo-NHS ester

Product#: POSN1604

$3,212.00

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ICG Sulfo-NHS ester

Cat. No. List below

Description

ICG Sulfo-NHS ester is a powerful and versatile near-infrared (NIR) fluorescent dye widely used in bioimaging applications. This amine-reactive form of indocyanine green (ICG) offers several advantages that make it an excellent choice for researchers in various fields.

ICG Sulfo-NHS ester readily reacts with amine groups on various biomolecules, including antibodies, peptides, proteins, ligands, and amine-modified oligonucleotides.

This reactivity allows for the formation of stable amide bonds between the dye and target molecules, particularly with the ε-amino groups of lysine residues or the amine terminus of nucleotides. ICG Sulfo-NHS ester can be excited using 750-800 nm laser lines or LEDs, offering flexibility in imaging setups. Its optical properties make it well-suited for various NIR imaging applications.

ICG is FDA-approved for certain medical diagnostics, which can facilitate its use in translational research. Some key applications include breast cancer sentinel lymph node navigation, detection of cerebral vessels, coronary arteries, & biliary trees, tumor localization & tracking, and identification of small hepatocellular carcinomas.

ICG Sulfo-NHS ester is a powerful tool for researchers in fields ranging from molecular biology to clinical diagnostics. Its unique combination of NIR fluorescence, water solubility, and versatile conjugation chemistry makes it an excellent choice for developing targeted imaging probes and studying biological processes in deep tissues.

What are the advantages?
1. Deep Tissue Imaging: The NIR fluorescence of ICG Sulfo-NHS ester allows for deep tissue imaging, penetrating further than visible light fluorophores. This property enables researchers to observe structures and processes beneath the skin surface, making it invaluable for in vivo imaging studies.
2. Enhanced Water Solubility: The sulfo-NHS ester group confers higher water solubility compared to standard NHS esters. This eliminates the need for organic co-solvents, simplifying the conjugation process and improving compatibility with biological systems

Specifications

Fluorophore: ICG
Reactive group: Sulfo-NHS ester
Excitation/Emission Max.(nm): 785/812
Extinction coefficient: ≥ 176,000 cm-1M-1
CF280: 0.05
Appearance: Green Solid
Molecular Weight: 908.09 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

ICG Dyes

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
RFP0815	ICG	RFP0915	ICG (water-soluble)
POS1604	ICG NHS ester	POA1616	ICG Vinylsulfone
POSN1604	ICG Sulfo-NHS ester	POI1616	ICG Isothiocyanate
POC1616	ICG Carboxylic acid	PWM1301	ICG Maleimid
POE1616	ICG Amine	PWT1301	ICG Thiol
POR2616	ICG Dichlorotriazine	POH1616	ICG Hydrazide
POK1616	ICG Alkyne	POG1616	ICG PEG4-Alkyne
DOC1061	ICG ADIBO	POZ1616	ICG Azide

Background

ICG Dyes

ICG is clinically approved NIR dye and used in medical diagnostics, in vitro, vivo and animal model study. NIR fluorescence allows to observe the deep image from the surface of skin and its usage spreads to a wide range of research fields. ICG fluorescence method is safe and cost effective technique and used in a wide range of medical fields such as breast cancer sentinel lymph node navigation, detecting cerebral vessels, coronary arteries and biliary trees, tracking tumor location, detecting small HCC, etc. The method has several advantages such as radiation free, compact instrumentation, real-time monitoring, easy operation, etc. The maximum excitation/emission values of ICG are 785/821 nm. When ICG is injected into a human body, it rapidly bound to plasma protein, mainly high-density lipoprotein, and generates red-shifted fluorescence (845 nm). ICG in aqueous solution is unstable over time, thus the fresh solution should be used for effective trials. BioActs provides ICG dye for biological research and medical diagnostics and also offers various reactive and functionalized ICG dyes for labeling of antibodies, peptides, proteins and ligands.

Table 1. ICG dye applications

Figure 1. Structure of Indocyanine Green (ICG) dye

Citation & Reference

1. Masashi Gotoh. Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy. J Thorac Cardiovasc Surg 126.6 (2003): 1916-21.

2. Aaron M. Mohs. An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors. IEEE Trans Biomed Eng 62.5 (2015): 1416-24.

3. Mohammed Hassan. Near Infrared Fluorescence Imaging with ICG in TECAB Surgery Using the da Vinci Si Surgical System in a Canine Model. J Card Surg 27.2 (2012): 158-162.

4. R. C. Benson. Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23.1 (1978): 159-63.

5. Mitsuharu Miwa. The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal 2 (2010): 26-28.