Flamma® 496 ADIBO
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 496 ADIBO
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 494/520 nm
2. High extinction coefficient: ≥ 70,000 cm?¹M?¹
3. Low CF280: 0.12
4. ADIBO (aza-dibenzocyclooctyne) reactive group for strain-promoted azide-alkyne cycloaddition
5. Yellow solid appearance
6. Molecular weight: 672.63 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Strain-promoted azide-alkyne cycloaddition (SPAAC) reactions
2. Cellular imaging and detection
3. Nucleotide functionalization
4. Biomolecule labeling and tracking
5. Live-cell imaging studies
6. Fluorescence microscopy
7. Flow cytometry
8. Metabolic labeling
9. Peptide and protein engineering
Advantages:
1. Spectral similarity to popular dyes (Alexa488, FITC, Cy2)
2. Excellent optical properties when excited with a 488 nm laser line
3. Copper-free click chemistry for biocompatible labeling
4. Stable fluorescence signal for extended imaging sessions
5. High sensitivity for detecting low-abundance biological structures
6. No need for coupling reagents or catalysts
7. Minimal interference with native biochemical processes
8. Bioorthogonal labeling strategy
9. Compatibility with living systems
- Fluorophore: Flamma® Fluors 496
- Reactive group: ADIBO
- Excitation/Emission Max.(nm): 494/520
- Spectrally similar dyes: Alexa488, FITC, Cy2
- Extinction coefficient: ≥ 70,000 cm-1M-1
- CF280: 0.12
- Appearance: Yellow Solid
- Molecular Weight: 672.63 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| DWC1001 | Flamma® 496 ADIBO | 496 | 520 | Alexa488, FITC, Cy2 |
| DWC1011 | Flamma® 552 ADIBO | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| DWC1415 | Flamma® 581 ADIBO | 581 | 596 | Alexa594, DyLight594 |
| DWC1021 | Flamma® 648 ADIBO | 648 | 663 | Alexa647, DyLight650, Cy5 |
| DWC1051 | Flamma® 675 ADIBO | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| DWC1031 | Flamma® 749 ADIBO | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| DWC1061 | Flamma® 774 ADIBO | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.
