Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
PBS with 5% Nonfat Soy Milk is a specialized solution used primarily in Western blotting and other protein analysis techniques. Soy milk provides an alternative plant-based blocking agent for those avoiding animal products.Soymilk was found to have superior blocking efficacy compared to other blocking agents, including nonfat dry milk, in terms of achieving a higher signal-to-noise ratio [1]. Soymilk achieved effective blocking with very short blocking times, even as little as 10 seconds in some cases [1]. Soymilk is casein-free, biotin-free, and devoid of animal proteins [1]. This makes it advantageous in certain applications where these components might interfere. This can significantly reduce the overall time required for the Western blotting procedure. PBS helps maintain a stable pH, which is important for optimal antibody-antigen interactions and overall assay performance. The phosphate in PBS may interfere with the detection of phosphorylated proteins
* The optimal concentration and blocking time may vary depending on the specific experimental conditions and antibodies used.
Usage consideration:
Milk contains phosphatases that may interfere with phosphoprotein detection.
Soymilk is casein-free, biotin-free, and devoid of animal proteins, which can be advantageous in certain applications.
Applications
Block non-specific binding: The nonfat dry milk proteins occupy vacant binding sites on the membrane, reducing background noise and improving the signal-to-noise ratio in Western blots.
Dilute antibodies: It serves as a medium for diluting primary and secondary antibodies used in immunoblotting procedures.
Advantages:
Soymilk has been reported to have superior blocking efficacy compared to other blocking agents, including nonfat dry milk, in terms of higher signal-to-noise ratio.
Soymilk achieved effective blocking with very short blocking times, even as little as 10 seconds in some cases.
Soymilk performed better than nonfat dry milk on blots that were stripped for reuse.
Preservation: ProClin 300 helps maintain the integrity of the solution, preventing microbial growth.
Versatility: This formulation is compatible with a wide range of antibodies and target proteins
Composition:
Ingredients
Concentration
Nonfat Soy Milk
5%
ProClin 300
0.05%
Potassium Chloride
2.7 mM
Sodium Chloride
137 mM
Sodium Phosphate dibasic
10 mM
Potassium Phosphate monobasic
1.8 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
Reference:
[1] Galva, C., Gatto, C., & Milanick, M. (2012). Soymilk: an effective and inexpensive blocking agent for immunoblotting. Analytical biochemistry, 426(1), 22–23. https://doi.org/10.1016/j.ab.2012.03.028
