PBS with 5% Nonfat Soy Milk
PBS with 5% Nonfat Soy Milk
A research-grade phosphate-buffered saline blocking buffer formulated with 5% nonfat soy milk for Western blotting and immunoblotting workflows. Buffered to pH 7.4 with a defined PBS salt system. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
- 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications
- PBS base at pH 7.4 with 137 mM Sodium Chloride, 2.7 mM Potassium Chloride, 10 mM Sodium Phosphate dibasic, and 1.8 mM Potassium Phosphate monobasic
- 5% Nonfat Soy Milk blocking agent - casein-free, biotin-free, and free of animal proteins
- 0.05% ProClin 300 preservative protects solution integrity against microbial growth
- DNase- and RNase-activity tested (18 hr incubation, room temperature) - no activity detected
- Manufactured under ISO 13485-certified facilities; final packaging and QC performed at the DiagnoCine R&D and Quality Testing Center, Totowa, New Jersey, USA
- Customizable concentration, pH, and additive content available on request — support@diagnocine.com
- pH7.4
- AppearanceClear, Colorless Liquid
- Filtration0.1 µm membrane filtration (single step)
- DNase ActivityNone Detected
- RNase ActivityNone Detected
- Storage4°C
- Shelf Life1 Year
- Sodium Chloride137 mM
- Sodium Phosphate dibasic10 mM
- Nonfat Soy Milk5%
Engineered where standard blocking buffers fall short
Blocking buffers can carry particulates, drift in pH and ionic strength, and introduce inconsistent lot-to-lot background in sensitive imaging and blotting assays. These buffers are made from the water source through final fill to reduce these failure modes.
0.1 µm filtered
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Precise, stable pH
Formulated to pH 7.4 with a defined PBS salt system (NaCl, KCl, Na2HPO4, KH2PO4) supporting consistent antibody-antigen interactions.
Ultrapure-grade water
Prepared with ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> water-quality practice.
Low-background blocking chemistry
Soy milk-based blocking is casein-free, biotin-free, and free of animal proteins, supporting a higher signal-to-noise ratio in immunoblotting.[1]
Defined, traceable composition
Every buffer salt, blocking agent, and preservative is documented by concentration for lot-level traceability.
Customization on demand
pH, molarity, blocking agent concentration, and additive content can be adjusted on request.
0.1 µm membrane filtration
DCP-DMB06 is filtered through a 0.1 µm membrane. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
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1
0.1 µm Membrane Filtration
Removes large particulates and aggregates, helping protect downstream applications.
Filtration
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
© Diagnocine® — DCP-DMB06
Where PBS with 5% Nonfat Soy Milk performs
Developed primarily as a blocking and antibody-dilution buffer for Western blotting and related protein-detection workflows.
Dot Blot & Protein Arrays
Blocks unoccupied binding sites on nitrocellulose or PVDF membranes in dot blot and protein array formats to reduce non-specific antibody binding.
Wash, Dilution & Reconstitution
A stable, defined-pH PBS system suitable for washing membranes and diluting reagents used in downstream analysis.
iPSC-Derived Model Handling
Defined PBS chemistry supports washing and buffer-exchange steps around sensitive iPSC-derived cell models.
Endothelial & Primary Cell Studies
Defined ionic composition supports buffer needs around endothelial and primary cell-based studies.
Western Blotting, ELISA & Blocking
The primary application of this product: soy milk-based blocking of non-specific binding sites and dilution of primary and secondary antibodies in immunoblotting.[1]
Microscopy & Optical Sensing
0.1 µm-filtered composition supports use as a wash/dilution buffer around optical and biosensor workflows.
Detailed product specifications
Specifications reflect the composition and quality control data documented for DCP-DMB06.
| Parameter | Specification |
|---|---|
| Formulation | PBS with 5% Nonfat Soy Milk blocking buffer |
| Appearance | Clear, Colorless Liquid |
| pH USP <791> | 7.4 |
| Sodium Chloride (NaCl) | 137 mM |
| Potassium Chloride (KCl) | 2.7 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 1.8 mM |
| Nonfat Soy Milk | 5% |
| ProClin 300 (preservative) | 0.05% |
| Parameter | Specification |
|---|---|
| Sterility | Nonsterile |
| DNase Activity | None detected (18 hr incubation with plasmid DNA, room temperature) |
| RNase Activity | None detected (18 hr incubation with ribosomal RNA, room temperature) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified facilities |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485 | ISO 13485-certified facilities |
| Regulatory Alignment | ISO 13485:2016 |
| Production Method | Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization and assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans. |
Full composition
Every component and concentration below is reported exactly as documented for this lot type; contact support@diagnocine.com for lot-specific Certificates of Analysis.
| Component | CAS Number | Concentration |
|---|---|---|
| Nonfat Soy Milk | 5% | |
| ProClin 300 | 0.05% | |
| Potassium Chloride (KCl) | 7447-40-7 | 2.7 mM |
| Sodium Chloride (NaCl) | 7647-14-5 | 137 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 7558-79-4 | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 7778-77-0 | 1.8 mM |
Manufacturing & compliance
DCP-DMB06 is manufactured, filtered, and packaged under a controlled quality system with lot-level testing.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified facilities (suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated using ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> practice.
Fill & Finish
0.1 µm membrane filtration (single step).
Micro-Batch Precision
Final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA.
DNase Activity QC Tested
None detected after 18 hr incubation of plasmid DNA with this product at room temperature.
RNase Activity QC Tested
No RNase activity detected after 18 hr incubation of ribosomal RNA with this product at room temperature.
Sterility
Nonsterile.
Documentation / CoA
A Certificate of Analysis documenting appearance, pH, and DNase/RNase results is available on request.
How DCP-DMB06 compares
A comparison of DCP-DMB06 against conventional blocking buffers.
| Parameter | DCP-DMB06 | Conventional | Standard alternative |
|---|---|---|---|
| Blocking agent | 5% Nonfat Soy Milk (casein-free, biotin-free) | Nonfat dry milk | Nonfat dry milk |
| DNase/RNase activity tested | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Not specified | Not specified |
| Manufacturing QMS | ISO 13485-certified | Not specified | Not specified |
| Preservative included | check_circle ProClin 300 | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-DMB06, PBS with 5% Nonfat Soy Milk.
Supporting literature
Citations directly relevant to this product, followed by curated supporting literature.
Citations
- Galva, C., Gatto, C., & Milanick, M. (2012). Soymilk: an effective and inexpensive blocking agent for immunoblotting. Analytical Biochemistry, 426(1), 22-23. doi:10.1016/j.ab.2012.03.028
Supporting Literature
- Mahmood, T., & Yang, P. C. (2012). Western blot: technique, theory, and trouble shooting. North American Journal of Medical Sciences, 4(9), 429-434. doi:10.4103/1947-2714.100998
- Kurien, B. T., & Scofield, R. H. (2015). A brief review of other notable protein blotting methods. Methods in Molecular Biology, 1312, 367-380. doi:10.1007/978-1-4939-2694-7_37
- Good, N. E., et al. (1966). Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467-477. doi:10.1021/bi00866a011
