TBST with 5% Nonfat Dry Milk
TBST with 5% Nonfat Dry Milk
A ready-to-use Tris-buffered saline blocking buffer formulated with 5% Nonfat Dry Milk and 0.05% Tween-20 for Western blotting and other protein-detection workflows. Buffered to pH 7.4 with 20 mM Tris-Base and 150 mM Sodium Chloride, the formulation is supplied nonsterile. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
- 0.1 µm membrane filtration for a clean, ready-to-use blocking buffer (nonsterile)
- Buffered to pH 7.4 with 20 mM Tris-Base and 150 mM Sodium Chloride for stable antibody-antigen interactions
- 5% Nonfat Dry Milk formulation reduces background banding across phospho-specific and total antibodies
- 0.05% Tween-20 detergent minimizes non-specific antibody binding
- DNase- and RNase-activity tested, with none detected after 18 hr incubation at room temperature
- Preserved with 0.05% ProClin 300 for a 1-year shelf life at 4°C
- Manufactured under ISO 13485-certified facilities
- Custom concentrations, additives, and pH formulations available on request
- pH7.4
- Tris-Base20 mM
- Sodium Chloride150 mM
- Nonfat Dry Milk5%
- Tween-200.05%
- ProClin 300 (Preservative)0.05%
- Filtration0.1 µm membrane filtration (single step)
- SterilityNonsterile
- Storage4°C
- Shelf Life1 year
Engineered where standard blocking buffers fail
Conventional milk-blocking buffers are often hand-mixed on the bench: inconsistent milk hydration, unfiltered particulates that clog and stain membranes, undefined pH, no nuclease testing, and no preservative to control microbial growth once opened. TBST with 5% Nonfat Dry Milk removes that variability with a defined, filtered, preserved, and quality-tested formulation.
0.1 µm filtered
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Stable, Buffered pH
20 mM Tris-Base and 150 mM Sodium Chloride hold the solution at pH 7.4 for consistent antibody-antigen interactions across runs.
Nuclease-Tested Formulation
No DNase activity and no RNase activity were detected after 18 hr incubation with plasmid DNA and ribosomal RNA, respectively, at room temperature.
Low-Background Detection
5% Nonfat Dry Milk plus 0.05% Tween-20 occupy non-specific binding sites and reduce background, improving signal-to-noise on Western blots.
Defined, Traceable Composition
Every component — Tris-Base, Sodium Chloride, Tween-20, Nonfat Dry Milk, and ProClin 300 — is formulated to a fixed concentration and preserved for shelf stability.
Customization on Demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, or a different pH can be formulated on request.
0.1 µm membrane filtration
DCP-DMB04 is passed once through a 0.1 µm membrane. This removes large particulates and aggregates, helping protect downstream applications. The product is supplied nonsterile for use as a bench blocking and antibody-dilution buffer.
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0.1 µm Membrane Filtration
Removes large particulates and aggregates, helping protect downstream applications.
0.1 µm membrane filtration
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
© Diagnocine® — DCP-DMB04
Where TBST with 5% Nonfat Dry Milk performs
Formulated as a blocking and antibody-dilution buffer for Western blotting and related protein-detection assays, with milk proteins that reduce background banding across a wide range of antibodies.
Dot Blot & Protein Arrays
Blocks unoccupied binding sites on nitrocellulose or PVDF membranes before antibody probing in dot blots and protein arrays.
Antibody Dilution & Blot Washing
Serves as a dilution medium for primary and secondary antibodies and as a wash/dilution buffer in immunoblotting procedures.
iPSC-Derived Lysate Analysis
Blocking and antibody dilution support for Western blot validation of marker proteins in iPSC-derived cell lysates.
Endothelial Signaling Protein Detection
Reduces background in Western blot detection of endothelial and primary cell signaling proteins.
Western Blot Blocking & Detection
Nonfat dry milk proteins occupy vacant binding sites on the membrane, reducing background noise and improving signal-to-noise ratio.
Downstream Target Validation
Supports Western blot confirmation of protein targets identified in live-cell imaging and biosensor experiments.
Product specifications
All values below reflect this product’s own formulation and quality-control results as documented for DCP-DMB04.
| Parameter | Specification |
|---|---|
| Formulation | TBST + 5% Nonfat Dry Milk |
| Appearance | Clear, Colorless Liquid |
| pH | 7.4 |
| Tris-Base concentration | 20 mM |
| Sodium Chloride concentration | 150 mM |
| Tween-20 concentration | 0.05% |
| Nonfat Dry Milk concentration | 5% |
| Parameter | Specification |
|---|---|
| Sterility | Nonsterile |
| Filtration | 0.1 µm membrane filtration (single step) |
| DNase activity | None detected (18 hr, room temperature, plasmid DNA) |
| RNase activity | None detected (18 hr, room temperature, ribosomal RNA) |
| Preservative | ProClin 300, 0.05% |
| Parameter | Specification |
|---|---|
| Storage temperature | 4°C (should always remain cold) |
| Shelf life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing facility | ISO 13485-certified (Suppliers of DiagnoCine Precision) ISO 13485 |
| Final packaging & QA | DiagnoCine R&D and Quality Testing Center |
| Custom assembly location | Totowa, New Jersey, USA |
| Intended use | Research Use Only (RUO) |
| Customization | Available on inquiry (concentrations, additives, pH) |
Full composition
Complete component list for TBST with 5% Nonfat Dry Milk, with concentrations as formulated.
| Component | CAS Number | Concentration |
|---|---|---|
| Nonfat Dry Milk | 5% | |
| ProClin 300 | 0.05% | |
| Tris-Base | 77-86-1 | 20 mM |
| Sodium Chloride | 7647-14-5 | 150 mM |
| Tween-20 | 9005-64-5 | 0.05% |
Manufacturing & compliance
DCP-DMB04 is manufactured, packaged, and tested under a controlled quality system spanning ISO 13485-certified supplier facilities and DiagnoCine’s own R&D and Quality Testing Center.
ISO 13485:2016 Quality System
Manufactured under ISO 13485-certified facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated using ultrapure Type 1 water (18.2 MΩ·cm).
Dedicated QA & R&D Testing
Final packaging, quality assurance, and testing are completed at the DiagnoCine R&D and Quality Testing Center.
Custom Micro-Batch Assembly
Specific customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
Nuclease Testing (DNase/RNase)
No DNase or RNase activity was detected after 18 hr incubation with plasmid DNA and ribosomal RNA, respectively, at room temperature.
Preservative Stability (ProClin 300)
0.05% ProClin 300 is included to extend shelf life across the 1-year storage period at 4°C.
pH Verification
Buffer is verified to pH 7.4 using 20 mM Tris-Base and 150 mM Sodium Chloride.
Documentation / CoA
QC results including appearance, pH, and nuclease testing are documented at the DiagnoCine R&D and Quality Testing Center.
How DCP-DMB04 compares
A comparison against a typical hand-mixed TBST-milk blocking buffer and a generic commercial alternative.
| Parameter | DCP-DMB04 | Hand-Mixed Buffer | Generic Alternative |
|---|---|---|---|
| Nonfat dry milk concentration | Precise, fixed 5% | Variable, operator-dependent | Variable |
| Filtration | 0.1 µm membrane filtration | Typically unfiltered | Typically unfiltered |
| Nuclease testing | check_circle DNase/RNase tested | cancel | cancel |
| Preservative included | check_circle ProClin 300, 0.05% | cancel | Varies |
| Buffered pH | check_circle pH 7.4 | cancel Not specified | cancel Not specified |
| Manufacturing QMS | check_circle ISO 13485-certified | cancel | cancel |
| Custom formulation | check_circle Available | cancel | cancel |
| Ready-to-use formulation | check_circle Pre-mixed | cancel Requires manual preparation | cancel Requires manual preparation |
Frequently asked questions
Common questions about DCP-DMB04, TBST with 5% Nonfat Dry Milk.
Supporting literature
Curated literature relevant to milk-based blocking buffers, Tris-buffered saline chemistry, and immunoblotting technique.
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012;4(9):429-434. doi:10.4103/1947-2714.100998
- Kurien BT, Scofield RH. Western blotting. Methods. 2006;38(4):283-293. doi:10.1016/j.ymeth.2005.11.007
- Kurien BT, Scofield RH. Common artifacts and problems in immunoblotting. Adv Exp Med Biol. 2015;869:517-524. doi:10.1007/978-1-4939-2599-1_37
- Alegria-Schaffer A, Lodge A, Vattem K. Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 2009;463:573-599. doi:10.1016/S0076-6879(09)63033-0
- Gordon JA, Balbach JJ. The effect of blocking agents on antibody binding: a comparative study. Anal Biochem. 1990;191(2):228-233. doi:10.1016/0003-2697(90)90212-2
- Bass JJ, Wilkinson DJ, Rankin D, et al. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017;27(1):4-25. doi:10.1111/sms.12702
- Hnasko TS, Hnasko RM. The Western blot. Methods Mol Biol. 2015;1318:87-96. doi:10.1007/978-1-4939-2742-5_9
- Ivell R, Teerds K, Hoffman GE. Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them. Endocrinology. 2014;155(3):676-687. doi:10.1210/en.2013-1971
- Junker B. Detergents: an overview. Methods Enzymol. 2009;463:27-35. doi:10.1016/S0076-6879(09)63003-2