TBST with 5% Nonfat Dry Milk
Cat No.: DCP-DMB04
Size: 100 mL, 500 mL & 1000 mL
Non-Sterile; Ultrapure, Filtered 1-micron membrane ONCE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
TBST with 5% Nonfat Dry Milk is a specialized solution used primarily in Western blotting and other protein analysis techniques. Milk contains many proteins of various sizes, giving a better chance to reduce background banding compared to single-protein solutions like BSA. It works well with a wide range of antibodies, including many phospho-specific and total antibodies. TBS helps maintain a stable pH, which is important for optimal antibody-antigen interactions and overall assay performance. TBS is recommended when using alkaline phosphatase (AP) conjugates or AP-based detection methods, as the phosphate in PBS can interfere with AP activity. Tween 20 helps reduce background and non-specific binding of antibodies. It acts as a detergent to decrease non-specific interactions. The influence of Tween 20 can be epitope-specific. For some antibodies, it may enhance binding, while for others it may reduce binding.
* The optimal concentration and blocking time may vary depending on the specific experimental conditions and antibodies used.
Usage consideration:
- When using biotin-conjugated antibodies or avidin-biotin detection systems, milk should be avoided due to its intrinsic biotin content.
- For certain specialized applications or problematic antibodies, alternative blocking agents may be necessary.
- Milk contains casein, which is a phosphoprotein. This can potentially interfere with the detection of phosphorylated proteins in some cases.
- For PVDF membranes, it may be better to avoid Tween-20 in the antibody dilution buffer when using milk as a blocker, as this can increase background.
Applications
- Block non-specific binding: The nonfat dry milk proteins occupy vacant binding sites on the membrane, reducing background noise and improving the signal-to-noise ratio in Western blots.
- Dilute antibodies: It serves as a medium for diluting primary and secondary antibodies used in immunoblotting procedures.
Advantages:
- Effective Blocking: Nonfat dry milk is an excellent and cost-effective blocking agent for most applications.
- Preservative: ProClin 300 acts as a preservative, extending the shelf life of the solution.
- Detergent: Tween-20 in TBST helps reduce background and non-specific binding.
Composition:
| Ingredients |
Concentration |
| Nonfat Dry Milk |
5% |
| ProClin 300 |
0.05% |
| Tris-Base |
20 mM |
| Sodium Chloride |
150 mM |
| Tween-20 |
0.05% |
Quality Control:
- Appearance: Clear, Colorless Liquid.
- pH: 7.4
- Sterility: Filtered 1 micron once in a non-sterile environment.
- DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: 4 ℃ (It should always remain cold)
- Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
