TBS with 5% Nonfat Soy Milk
TBS with 5% Nonfat Soy Milk
A research-grade, Tris-buffered saline blocking and dilution buffer formulated with 5% nonfat soy milk for Western blotting and immunoassay workflows. Buffered at pH 7.4 with 20 mM Tris-Base and 150 mM Sodium Chloride. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
- 0.1 µm membrane filtration (single step)
- Tris-buffered saline base: 20 mM Tris-Base, 150 mM Sodium Chloride, pH 7.4
- 5% Nonfat Soy Milk blocking agent — casein-free, biotin-free, animal-protein-free
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
- DNase- and RNase-tested: none detected after 18 hr incubation at room temperature
- Manufactured under ISO 13485-certified facilities
- Customizable concentration, pH, and additive content available on request
- pH7.4
- Tris-Base20 mM
- Sodium Chloride150 mM
- Nonfat Soy Milk5%
- ProClin 300 (Preservative)0.05%
- Filtration0.1 µm membrane filtration (single step)
- AppearanceClear, Colorless Liquid
- Storage4°C
- Shelf Life1 year
- SterilityNonsterile
Engineered where standard blocking buffers fall short
Conventional blocking buffers can carry residual particulates, batch-to-batch pH drift, and animal-derived proteins that interfere with sensitive detection chemistries. TBS with 5% Nonfat Soy Milk is designed to address each of these issues.
0.1 µm filtered
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Precise, stable pH
Formulated at pH 7.4 with 20 mM Tris-Base and 150 mM Sodium Chloride to support optimal antibody-antigen interactions and reproducible blot-to-blot performance.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base for a low-background, reproducible buffer system.
Low-background blocking
Soymilk-based blocking has been reported to achieve a higher signal-to-noise ratio than nonfat dry milk, with effective blocking possible in as little as 10 seconds in some cases.[1]
Defined, traceable composition
Nonfat Soy Milk, ProClin 300, Tris-Base, and Sodium Chloride are each present at a controlled, stated concentration for consistent performance lot to lot.
Customization on demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, different pH, or other modifications can be produced on request.
0.1 µm membrane filtration
DCP-DMB05 is filtered through a 0.1 µm membrane before final fill.
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1
0.1 µm Membrane Filtration
Removes large particulates and aggregates, helping protect downstream applications.
Filtration
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
© Diagnocine® — DCP-DMB05
Where DCP-DMB05 is used
A Tris-buffered, soy-milk-based blocking and dilution buffer for Western blotting, immunoblotting, and related detection workflows.
Dot Blot & Protein Arrays
Blocks unoccupied binding sites on nitrocellulose or PVDF membranes before antibody probing in dot blot and protein array formats.
Wash, Dilution & Reconstitution
Serves as a medium for diluting primary and secondary antibodies used in immunoblotting procedures.
iPSC-Derived Model Handling
Tris-buffered saline base compatible with wash and dilution steps used alongside iPSC-derived model workflows.
Endothelial & Primary Cell Models
Low-particulate buffer suitable for wash and dilution steps with primary and endothelial cell models.
ELISA, Blotting & Blocking
Blocks non-specific binding sites on the membrane, reducing background noise and improving the signal-to-noise ratio in Western blots; TBS is recommended when using alkaline phosphatase (AP) conjugates since phosphate in PBS can interfere with AP activity.
Microscopy & Optical Sensing
Low-particulate, 0.1 µm-filtered formulation suited to imaging and optical sensing workflows.
Product specifications
Measured and declared values for DCP-DMB05, as released by Diagnocine.
| Parameter | Specification |
|---|---|
| Formulation | Tris-Buffered Saline with 5% Nonfat Soy Milk |
| Appearance | Clear, Colorless Liquid |
| pH USP <791> | 7.4 |
| Tris-Base | 20 mM |
| Sodium Chloride | 150 mM |
| Nonfat Soy Milk | 5% |
| ProClin 300 | 0.05% |
| Parameter | Specification |
|---|---|
| Sterility / Filtration | Nonsterile; 0.1 µm membrane filtration (single step) |
| DNase Activity | None detected (plasmid DNA, 18 hr, room temperature) |
| RNase Activity | None detected (ribosomal RNA, 18 hr, room temperature) |
| Water Quality | Ultrapure Type 1 Water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-Certified Facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-Certified |
| Production Method | Final packaging, QA, and testing at the Diagnocine R&D and Quality Testing Center; customization and assembly at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Every component of DCP-DMB05 with its stated concentration, as released per lot.
| Component | CAS Number | Concentration |
|---|---|---|
| Nonfat Soy Milk | 5% | |
| ProClin 300 | 0.05% | |
| Tris-Base | 77-86-1 | 20 mM |
| Sodium Chloride | 7647-14-5 | 150 mM |
Manufacturing & compliance
DCP-DMB05 is produced and released under a controlled quality system with defined testing at each stage.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified supplier facilities.
Ultrapure Type 1 Water
Prepared using Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base.
Controlled Fill & Finish
Final packaging, quality assurance, and testing are performed at the Diagnocine R&D and Quality Testing Center.
Micro-Batch Precision
Customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.
DNase Activity Testing
None detected after incubation of plasmid DNA with this product for 18 hr at room temperature.
RNase Activity Testing
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hr at room temperature.
Sterility & Filtration
Nonsterile; 0.1 µm membrane filtration (single step).
Documentation / CoA
A Certificate of Analysis is available on request for lot-specific release data.
How DCP-DMB05 compares
A side-by-side view of DCP-DMB05 against conventional dairy-milk-based TBS blockers and standard PBS-based blocking buffers.
| Parameter | DCP-DMB05 | Conventional TBS + Dry Milk Blocker | Standard PBS-Based Blocking Buffer |
|---|---|---|---|
| Animal-Protein-Free Blocking Agent | check_circle | cancel | cancel |
| Casein-Free Formulation | check_circle | cancel | cancel |
| Biotin-Free Formulation | check_circle | cancel | cancel |
| Compatible with Alkaline Phosphatase (AP) Detection | check_circle | check_circle | cancel |
| DNase / RNase Tested | check_circle | cancel | cancel |
| ISO 13485-Certified Manufacturing | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-DMB05, TBS with 5% Nonfat Soy Milk.
Supporting literature
Citations and curated literature relevant to soymilk-based blocking and Tris-buffered saline chemistry.
Citations
- Galva, C., Gatto, C., & Milanick, M. (2012). Soymilk: an effective and inexpensive blocking agent for immunoblotting. Analytical Biochemistry, 426(1), 22-23. doi:10.1016/j.ab.2012.03.028
Supporting Literature
- Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, 76(9), 4350-4354. doi:10.1073/pnas.76.9.4350
- Mahmood, T., & Yang, P. C. (2012). Western blot: technique, theory, and trouble shooting. North American Journal of Medical Sciences, 4(9), 429-434. doi:10.4103/1947-2714.100998
- Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., & Singh, R. M. (1966). Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467-477. doi:10.1021/bi00866a011