PBST with 5% Nonfat Dry Milk
Cat No.: DCP-DMB03
Size: 100 mL, 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
PBST with 5% Nonfat Dry Milk is a commonly used blocking and antibody dilution solution for Western blotting. Milk contains many proteins of various sizes, giving a better chance to reduce background banding compared to single-protein solutions like BSA. It works well with a wide range of antibodies, including many phospho-specific and total antibodies. PBS helps maintain a stable pH, which is important for optimal antibody-antigen interactions and overall assay performance. The phosphate in PBS may interfere with the detection of phosphorylated proteins. Tween-20 in PBST helps reduce background and non-specific binding.
* The optimal concentration and blocking time may vary depending on the specific experimental conditions and antibodies used.
Usage consideration:
- Not recommended for detecting phosphorylated proteins due to potential interference from phosphate in PBS.
- May interfere with biotin-based detection systems due to endogenous biotin in milk.
Applications
- Block non-specific binding: The nonfat dry milk proteins occupy vacant binding sites on the membrane, reducing background noise and improving the signal-to-noise ratio in Western blots.
- Dilute antibodies: It serves as a medium for diluting primary and secondary antibodies used in immunoblotting procedures.
Advantages:
- Effective Blocking: Nonfat dry milk is an excellent and cost-effective blocking agent for most applications4.
- Preservative: ProClin 300 acts as a preservative, extending the shelf life of the solution12.
- Detergent: Tween-20 in PBST helps reduce background and non-specific binding.
Composition:
| Ingredients |
Concentration |
| Nonfat Dry Milk |
5% |
| ProClin 300 |
0.05% |
| Potassium Chloride |
2.7 mM |
| Sodium Chloride |
137 mM |
| Sodium Phosphate dibasic |
10 mM |
| Potassium Phosphate monobasic |
1.8 mM |
| Tween-20 |
0.05% |
Quality Control:
- Appearance: Clear, Colorless Liquid.
- pH: 7.4
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
- DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: 4 ℃
- Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
