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Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit
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Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit
Competitive quantitative immunoassay for apolipoprotein B100 (Apo-B100) in rabbit serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | apolipoprotein B100 (Apo-B100) |
| Uniprot No. | P17165 |
| Species | Oryctolagus cuniculus (Rabbit) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 78 ng/mL-5000 ng/mL |
| Sensitivity | 19.5 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Competitive (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cardiovascular |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Competitive binding
450 nm detection
Standard curve
Quantification
In this competitive ELISA, free apolipoprotein B100 (Apo-B100) in the sample competes with plate-bound antigen for antibody binding. Signal intensity is inversely proportional to analyte concentration. Final quantification is derived from a standard curve fitted using Curve Expert software, covering 78 ng/mL-5000 ng/mL with a minimum detectable dose of 19.5 ng/mL.
This Rabbit Apo-B100 ELISA Kit was designed for the quantitative measurement of Rabbit Apo-B100 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.
STRING: 9986.ENSOCUP00000004631
UniGene: Ocu.1828
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 98-108 | |||||
| 1:800 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 87 | 82-92 | ||||
