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Human Eotaxin 2/CCL24 ELISA Kit
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Human Eotaxin 2/CCL24 ELISA Kit
Sandwich quantitative immunoassay for chemokine (C-C motif) ligand 24 in human serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | chemokine (C-C motif) ligand 24 |
| Uniprot No. | O00175 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 78.125 pg/mL-5000 pg/mL |
| Sensitivity | 67.660 pg/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
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Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, chemokine (C-C motif) ligand 24 in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 78.125 pg/mL-5000 pg/mL with a minimum detectable dose of 67.660 pg/mL.
This human CCL24 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL24 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL24 levels in the sample and is measured at 450 nm through a microplate reader.
CCL24, also known as eotaxin2, mainly chemoattracts eosinophils. It can promote the separation of eosinophils from endothelial cells and then combine with its sole ligand to promote eosinophils to enter the tissue. CCL24 promotes cell trafficking and regulates inflammatory and fibrotic activities mainly through the CCR3. CCL24 induces chemotaxis and activation of CCR3-expressing cells, including immune cells and fibroblasts. A study found that CCL24 stimulated human lung fibroblast proliferation and collagen synthesis and was associated with the progression of idiopathic pulmonary fibrosis. Suppressing the expression of CCL24 can reduce airway inflammation. Macrophages can also selectively induce the production of CCL24.
- This protein is an eosinophil chemoattractant that is up-regulated by human rhinovirus infection in cultured bronchial epithelial cells. PMID: 11467997
- alters eosinophil integrin function via mitogen-activated protein kinases PMID: 12034562
- Intradermal injection of CCL24 induces recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions in atopic and nonatopic volunteers. PMID: 12193745
- Plasma eotaxin-2 concentrations differed in asthmatic patients with respect to aspirin intolerance and tolerance, indicating that eotaxin-2 may be differentially up-regulated according to aspirin intolerance. PMID: 16304252
- Our results suggest that Eo2 +179T>C and Eo2 +275C>T of eotaxin-2 might be associated with the susceptibility of ulcerative colitis. PMID: 16391516
- These data support the idea that CCL24/eotaxin-2 is part of the mechanism of CD4 lymphocyte activation paracrinally induced by Nef. PMID: 17630924
- eotaxin-2 is a chemokine strongly associated with primary and metastatic tumors of colorectal origin PMID: 17908961
- the ability to produce eotaxin-2/CCL24 is acquired during the differentiation into eosinophilic lineage which is dependent on GATA-1 expression PMID: 17917245
- stimulates lung fibroblast proliferation and collagen synthesis PMID: 20143648
- CCR3 is differentially expressed on inflammatory cells in rheumatoid arthritis, while eotaxin-2, a potent CCR3 agonist, is differentially expressed in active disease. PMID: 20659406
- Phosphodiesterase 4 inhibitors, rolipram and RO-20-1724 have no effect on CCL24 expression in human primary bronchial epithelial cells. PMID: 22946025
- Study shows that CCL24 levels were significantly increased in age-related macular degeneration (AMD) patients despite Avastin treatment as compared with normal controls and those without Avastin. PMID: 23025269
- Pregnancy associated environments increased local CCL24/CCR3, supporting the process of decidualization in human early pregnancy. PMID: 23696919
- these data suggest that CCL26 and CCL24 are likely involved in the pathogenesis of chronic nasal hypereosinophilia, with a complex cooperation and different involvement of the various members of eotaxin family. PMID: 24989688
- HMGB1 did not elicit chemotaxis of human eosinophils alone and had no effect in combination with the eosinophil chemotactic agent, eotaxin-2 (CCL24). PMID: 25774667
- Study showed that CCL24 was upregulated in hepatocellular carcinoma (HCC) tissues and correlated with poor prognosis in HCC patients . Also, CCL24 was associated with the metastatic potential of HCC cell lines and promoted proliferation, migration, and invasion. PMID: 28042950
- Mean eotaxin 2 concentrations in nasal fluid in patients with perennial allergic rhinitis and nonallergic and allergic chronic rhinosinusitis with nasal polyps patients were significantly higher in comparison to control subjects. PMID: 28587510
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 91-101 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||