ALPQuench-TBS

Cat No.: DCP-ALPTBS1X

Size: 100 mL

Non-Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and 0.04-micron membrane ONCE.

ALPQuench-TBS represents a significant advancement in alkaline phosphatase (ALP) signal management for Tris-buffered saline-based immunoassay systems, providing precise control in complex, multi-step immunolabeling protocols. This advanced formulation, engineered with a unique combination of enzyme inhibitors and stabilizing agents, effectively neutralizes ALP activity between multiple staining cycles without affecting sample integrity or subsequent antibody-antigen interactions. By eliminating unwanted ALP activity and minimizing background staining, ALPQuench-TBS significantly enhances the accuracy and reliability of multiplex immunohistochemistry and ELISA techniques. Especially valuable for protocols involving multiple ALP-conjugated antibodies, it enables clear, non-overlapping labeling of various targets within a single specimen. Its optimized composition not only improves staining specificity but also streamlines workflows, reducing overall assay duration and reagent usage, making it an essential tool for advanced immunological research.

Applications:

• Multiplex immunohistochemistry (mIHC) using sequential ALP-based staining cycles in TBS-based systems

• Multi-round immunofluorescence protocols requiring ALP inactivation between labeling steps

• ELISA procedures involving multiple ALP-conjugated antibodies or detection reagents

• Endogenous alkaline phosphatase suppression in tissues with high background activity (e.g., intestine, liver, kidney)

• Multi-round IHC protocols using same-species primary antibodies with ALP-conjugated secondaries

• Automated IHC/IF staining platforms requiring standardized ALP quenching steps

Advantages:

• Complete ALP Inactivation — neutralizes residual alkaline phosphatase activity between staining rounds, preventing signal overlap and cross-reactivity

• Sample Integrity Preserved — does not affect tissue morphology or interfere with subsequent antibody-antigen interactions

• Cleaner Results — minimizes non-specific background in both chromogenic and fluorescent detection systems

• TBS-Optimized — formulated in Tris-buffered saline for superior pH stability and compatibility with TBS-based immunoreagents

• Easy to Use — integrates seamlessly into existing multi-step protocols with no specialized equipment required

• Versatile — suitable for FFPE and frozen tissue sections as well as ELISA-based applications

• Workflow Efficiency — reduces overall assay duration and reagent consumption across multi-round labeling protocols

Composition:
 

Quality Control:

• Appearance: Clear, Colorless Liquid.

• pH: 7.4

• Sterility: Non-Sterile

Storage and Shelf Life:

• Storage: 4℃ 

• Shelf life: 1 year

CUSTOMIZATION:  Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.