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Rat TNF-α ELISA kit
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Rat TNF-α ELISA kit
Sandwich quantitative immunoassay for Rat TNF-α ELISA kit in rat tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | P16599 |
| Species | Rattus norvegicus (Rat) |
| Sample types | tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid |
| Detection range | 15.6 pg/mL-1000 pg/mL |
| Sensitivity | 3.9 pg/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Rat TNF-α ELISA kit in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 15.6 pg/mL-1000 pg/mL with a minimum detectable dose of 3.9 pg/mL.
This ELISA kit provides quantitative measurement of TNF (tumor necrosis factor) in Rattus norvegicus samples, supporting immunology research focused on innate immune activation and inflammatory signaling. Because TNF is a rapidly induced, short-lived cytokine whose concentrations can vary dramatically between baseline and inflammatory states, this kit is designed to capture that full dynamic window across multiple sample contexts.
Detection Range: The 15.6–1000 pg/mL range spans from near-baseline circulating TNF levels in unstimulated rat tissues to the elevated concentrations observed in LPS-challenged or sepsis-model animals, minimizing the need for serial dilutions while covering both resting and acutely inflamed conditions.
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
1000 | 2.717 | 2.634 | 2.675 | 2.487 | |||
500 | 1.476 | 1.467 | 1.471 | 1.283 | |||
250 | 0.853 | 0.908 | 0.881 | 0.693 | |||
125 | 0.608 | 0.629 | 0.619 | 0.431 | |||
62.5 | 0.395 | 0.381 | 0.388 | 0.200 | |||
31.2 | 0.281 | 0.293 | 0.287 | 0.099 | |||
15.6 | 0.218 | 0.207 | 0.213 | 0.025 | |||
0 | 0.191 | 0.185 | 0.188 |
| |||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
|
| Sample | cell culture supernates(n=4) | ||||
| 1:1 | Average % | 107 | ||||
| Range % | 99-113 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 96-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-109 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| cell culture supernates (n=5) | 94 | 89-100 | ||||
