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Rat Interleukin 1β,IL-1β ELISA Kit
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Rat Interleukin 1β,IL-1β ELISA Kit
Sandwich quantitative immunoassay for Rat Interleukin 1β,IL-1β in rat serum, plasma, tissue homogenates. available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | Q63264 |
| Species | Rattus norvegicus (Rat) |
| Sample types | serum, plasma, tissue homogenates. |
| Detection range | 0.156 ng/ml-10 ng/ml. |
| Sensitivity | 0.039 ng/ml. |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Rat Interleukin 1β,IL-1β in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.156 ng/ml-10 ng/ml. with a minimum detectable dose of 0.039 ng/ml..
Interleukin-1 beta (IL-1β) is a potent pro-inflammatory cytokine that plays a central role in immune responses and inflammatory processes. Activated macrophages, monocytes, and other immune cells produce this cytokine in response to pathogens and tissue damage. IL-1β acts as a key mediator in starting and amplifying inflammatory cascades. It influences fever induction, acute-phase protein synthesis, and T-helper cell activation. Dysregulation of IL-1β has been linked to numerous inflammatory diseases and autoimmune conditions, making it an important target for immunological research.
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||
10 | 2.903 | 2.834 | 2.868 | 2.689 | |||
5 | 2.208 | 2.167 | 2.187 | 2.008 | |||
2.5 | 1.563 | 1.508 | 1.536 | 1.356 | |||
1.25 | 0.872 | 0.859 | 0.865 | 0.686 | |||
0.625 | 0.579 | 0.551 | 0.565 | 0.386 | |||
0.312 | 0.363 | 0.353 | 0.358 | 0.178 | |||
0.156 | 0.284 | 0.274 | 0.279 | 0.099 | |||
0 | 0.184 | 0.175 | 0.180 |
| |||
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 88 | ||||
Range % | 80-96 | |||||
1:2 | Average % | 92 | ||||
Range % | 90-105 | |||||
1:4 | Average % | 94 | ||||
Range % | 91-103 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 | |||||
The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 99 | 90-105 | ||||
