FSD Fluor™ 647 NHS ester
Product#: KOSC1315
$3,943.36
Size of product (mg)
-
Trial
-
1 mg
-
5 mg
-
25 mg
Availability:
Ships in 1-2 Weeks
FSD Fluor™ 647 NHS ester
Cat. No. List below
Description
FSD Fluor™ 647 NHS ester is a next-generation, far-red fluorescent dye developed by BioActs. It offers superior optical properties compared to spectrally similar dyes, making it ideal for various biochemical and biological analytical applications.
FSD Fluor™ 647 NHS ester is optimized for labeling antibodies, peptides, proteins, ligands, and amplification substrates. These applications are particularly suited for cellular labeling and detection.
Elevate your fluorescence-based experiments with FSD Fluor™ 647 NHS ester and experience the difference in brightness, stability, and overall performance that only next-generation fluorophore technology can deliver. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule.
What are the advantages?
1. High Fluorescence Intensity: Excellent brightness after binding to biomolecules such as antibodies, nucleotides, and proteins.
2. Photostability: Enhanced stability for reliable and long-lasting fluorescence signals.
3. Single-Molecule Detection: Ideal for fluorescence correlation spectroscopy and fluorescence polarization measurements.
4. Reactivity: NHS esters readily react with amine-modified oligonucleotides and amino groups of proteins to form stable amide bonds.
Elevate your fluorescence-based experiments with FSD Fluor™ 647 NHS ester and experience the difference in brightness, stability, and overall performance that only next-generation fluorophore technology can deliver.
FSD Fluor™ 647 NHS ester offers superior fluorescence intensity compared to Alexa647 and DyLight650:
FSD Fluor™ 647 NHS ester is optimized for labeling antibodies, peptides, proteins, ligands, and amplification substrates. These applications are particularly suited for cellular labeling and detection.
Elevate your fluorescence-based experiments with FSD Fluor™ 647 NHS ester and experience the difference in brightness, stability, and overall performance that only next-generation fluorophore technology can deliver. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule.
What are the advantages?
1. High Fluorescence Intensity: Excellent brightness after binding to biomolecules such as antibodies, nucleotides, and proteins.
2. Photostability: Enhanced stability for reliable and long-lasting fluorescence signals.
3. Single-Molecule Detection: Ideal for fluorescence correlation spectroscopy and fluorescence polarization measurements.
4. Reactivity: NHS esters readily react with amine-modified oligonucleotides and amino groups of proteins to form stable amide bonds.
Elevate your fluorescence-based experiments with FSD Fluor™ 647 NHS ester and experience the difference in brightness, stability, and overall performance that only next-generation fluorophore technology can deliver.
FSD Fluor™ 647 NHS ester offers superior fluorescence intensity compared to Alexa647 and DyLight650:
| Feature | FSD Fluor™ 647 | Alexa647 / DyLight650 |
| Fluorescence Intensity | Up to 30% higher | Standard |
| Quantum Yield | Excellent | Good |
| Photostability | Enhanced | Standard |
| Low-Abundance Target Sensing | Higher sensitivity | Standard |
| Signal-to-Noise Ratio | Improved | Standard |
| Overall Performance | Next-generation performance | Well-established |
Specifications
- Fluorophore: FSD Fluor™ 647
- Reactive group: NHS ester
- Excitation/Emission Max.(nm): 651/667
- Spectrally similar dyes: Alexa647, DyLight650, Cy5
- Extinction coefficient: ≥ 217,000 cm-1M-1
- CF280: 0.03
- Appearance: Blue Liquid
- Molecular Weight: 1254.86 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm | Spectrally similar dyes |
| KOSC1002 | FSD Fluor™ 488 | 495 | 519 | Alexa Fluor 488, FAM, Cy2 |
| KOSC1003 | FSD Fluor™ 555 | 554 | 565 | Alexa Fluor 555, DyLight 549, Cy3, ATTO 550 |
| KOSC1001 | FSD Fluor™ 594 | 591 | 617 | Alexa Fluor 594, DyLight 594 |
| KOSC1315 | FSD Fluor™ 647 | 651 | 667 | Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650 |
| KOSC1515 | FSD Fluor™ 680 | 679 | 696 | Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680 |
| KOSC1702 | FSD Fluor™ 750 | 749 | 774 | Alexa Fluor 750, DyLight 755, Cy7, IRDye 750 |
| POSC1803 | FSD Fluor™ 800 | 774 | 790 | Cy7.5, DyLight 800, IRDye 800 |
Background
FSD Fluor™
FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.
Key Benefits:
- Superior brightness: up to 30% brighter than leading competitors, enabling detection fo low-abundance targets.
- Excellent Stability: Maintains high fluorescence intensity after bioconjugation
- Versatile Applications: Ideal for antibody labeling, nucleic acid detection, and protein studies
- Optimized for Single-Molecule Detection: Perfect for fluorescence correlation spectroscopy and polarization measurements
- Higher quantum yield comparing to traditional dyes
- Equipped with variety of reactive group
- Covering the full fluorescence spectral range from visible to NIR
- Enhanced Sensitivity: Detect targets with greater accuracy, even at low concentrations
- Reduced Background: Higher signal-to-noise ratio for clearer images and more reliable data
- Cost-Effective: Achieve superior results with less dye, maximizing your research budget
Figure 1. Fluorescence intensity comparison of FSD Fluor™ and other dyes
Figure 2. Fluorescence intensity comparison in varying dye/protein ratio
Figure 3. Immunofluorescence comparison of dye-antibody conjugates and photostability test of fluorescent secondary antibodies
Figure 4. Biodistribution images of FSD Fluor™ 750 and FSD Fluor™ 800
Citation & Reference
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