Eosin Y Solution
Cat No.: DCP-EY1X
Size: 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Aqueous Eosin Y 1% Solution is a pH-neutral, alcohol-free histological stain designed for laboratories requiring extended shelf life without compromising cytoplasmic staining clarity. Free of glacial acetic acid, this formulation ensures stable performance while retaining flexibility for on-demand acidification.
Key Features:
- Alcohol-free formulation: Minimizes tissue distortion, ideal for delicate samples (e.g., renal biopsies, cytology smears).
- No acetic acid: Maximizes shelf life and reduces precipitation risk.
- Pre-filtered: 0.04 µm membrane-filtered to remove particulates, ensuring stain clarity.
Applications:
1. Routine Histology
- H&E workflows: Counterstain nuclei post-hematoxylin with minimal cytoplasmic overstaining.
- Connective tissue studies: Highlights collagen, muscle, and elastic fibers in pink-red hues.
2. Specialized Techniques
- Immunohistochemistry (IHC): Compatible with chromogen-based protocols (e.g., DAB).
- Educational use: Ideal for teaching labs due to extended usability.
Composition:
| Ingredients |
Concentration |
| Eosin Y |
1% |
Usage Protocol:
-
Post-hematoxylin staining: Immerse slides for 1–3 minutes (adjust based on tissue type).
-
Rinse: Dip in distilled water for 5–10 seconds.
-
Optional acidification: For intensified staining, add 0.5 mL glacial acetic acid per 100 mL solution.
Quality Control:
- Appearance: Pink to orange-pink
- pH: 6.0–7.5 (unmodified)
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at room temperature away from bright light.
- Shelf life is 6 months unopened.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
