Yellow/Xylene Cyanol Loading Buffer [6X]
Cat No.: DCP-YCLB6X
Size: 5 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Yellow/Xylene Cyanol Loading Buffer [6X] is an essential component for DNA and RNA electrophoresis experiments. It facilitates the loading process by increasing the density of the sample and providing visual tracking. Tris-HCl provides a stable pH environment for the DNA/RNA samples and helps maintain the integrity of nucleic acids during electrophoresis. EDTA acts as a chelating agent and inhibits metal-dependent nucleases, protecting DNA/RNA from degradation. Tartrazine and Xylene Cyanol serves as a tracking dye and allows visual monitoring of the electrophoresis progress. The range between these two markers (approximately 100 bp and 10 kb) represents the size range of DNA fragments that can be effectively visualized using this loading dye. Glycerol increases the density of the sample and ensures that the DNA/RNA sample sinks to the bottom of the well when loaded.
* Please note that this buffer contains Tartrazine and Xylene Cyanol.
Advantages:
- Dual-color tracking of DNA migration
- Minimal interference with DNA band visualization
- Protection of DNA from nucleases
- Convenience of pre-mixed solution
Application:
- Agarose gel electrophoresis
- Preparation of DNA markers and samples
- Nucleic acid gel electrophoresis and blotting
Composition:
| Ingredients |
Concentration |
| Glycerol |
30% |
| Tartrazine |
0.3% |
| Xylene Cyanol FF |
0.03% |
| Tris-HCl |
10 mM |
| EDTA |
60mM |
Quality Control:
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Appearance: blue color.
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pH: 7.6
-
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
-
DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
-
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
-
Storage: -20 ℃
-
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
