Yellow/Xylene Cyanol Loading Buffer [6X]
FluxMPS™ Yellow/Xylene Cyanol Loading Buffer [6X]
An MPS-grade, ultrapure 6X DNA/RNA gel loading buffer engineered for microchannel-safe molecular biology work. Quadruple-stage filtration (0.1 µm membrane twice and 0.04 µm membrane twice) and a Tris-HCl/EDTA buffered, dual-dye (tartrazine and xylene cyanol FF) formulation deliver a stable pH 7.6, nuclease-tested loading solution for agarose gel electrophoresis, nucleic acid sizing, and lab-on-chip nucleic acid analysis workflows.
- Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice for an ultra-low particulate loading buffer
- Sterile, DNase- and RNase-tested formulation protects DNA/RNA integrity during electrophoresis
- Dual tartrazine and xylene cyanol FF tracking dyes for real-time visual monitoring of migration (approximately 100 bp to 10 kb range)
- Tris-HCl buffered system maintains a stable pH 7.6 for nucleic acid samples
- EDTA chelation inhibits metal-dependent nucleases
- Glycerol-based density formulation ensures reliable well loading
- Manufactured under ISO 13485-certified, CE-approved processes
- Custom dye, pH, and concentration formulations available on request
- pH7.6
- Formulation6X Concentrate
- AppearanceBlue color
- Tracking DyesTartrazine + Xylene Cyanol FF
- Tris-HCl10 mM
- EDTA60mM
- Filtration0.1 µm x2 + 0.04 µm x2
- DNase ActivityNone detected
- Storage-20°C
- Shelf Life1 year
Engineered where standard loading buffers fail
Conventional 0.22 µm-filtered loading buffers can carry subvisible particulates and residual nuclease activity that interfere with sensitive gel electrophoresis, blotting, and microfluidic nucleic acid analysis. DiagnoCine Precision positions this buffer as the world's cleanest choice for cell and molecular biology experiments, combining quadruple-stage filtration with a defined, nuclease-tested formulation.
Microchannel-safe purity
Final 0.04 µm membrane polish removes fine particulates that could otherwise clog microfluidic lanes or lab-on-chip electrophoresis channels.
Precise, stable pH
Tris-HCl buffering holds the formulation at pH 7.6, providing a stable environment that helps maintain nucleic acid integrity during loading and runs.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> water quality practices.
Low background for imaging & visualization
Dual tartrazine and xylene cyanol FF tracking dyes give clear visual monitoring of migration with minimal interference with DNA band visualization.
Defined, traceable composition
Every component — glycerol, tartrazine, xylene cyanol FF, Tris-HCl, and EDTA — is formulated to a stated concentration and QC-tested for nuclease activity.
Customization on demand
Alternate tracking dyes, concentrations, pH, and other modifications can be formulated on request.
Quadruple-stage filtration system
This loading buffer is filtered with a 0.1 µm membrane twice and a 0.04 µm membrane twice, delivering a sterile, ultra-low particulate solution for demanding molecular biology and microfluidic nucleic acid analysis workflows.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the life of downstream filtration stages.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden ahead of the final sterile passes.
-
3
0.1 µm Sterile-filtration I
Second-pass redundancy through the 0.1 µm membrane for consistent sterility assurance.
-
4
0.04 µm Sterile-filtration II — Final Polish
Final 0.04 µm polish performed in a sterile environment. The smallest mycoplasma types are approximately 0.2 microns, so DiagnoCine Precision's 0.1 µm/0.04 µm quadruple pass is designed to help prevent mycoplasma contamination of the buffer.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration passes remove finer particulates than a single conventional 0.22 µm pass, supporting cleaner loading, tracking, and downstream imaging of DNA and RNA fragments.
© Diagnocine® — DCP-YCLB6X
Where DCP-YCLB6X is used
A dual-dye, nuclease-tested loading buffer built for accurate visual tracking and clean gel loading across nucleic acid electrophoresis and microfluidic analysis workflows.
Automated Bioreactors & Robotics
For automated liquid-handling and robotic gel-loading platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer can be formulated on request to further protect sensitive valves, sensors, and dispensing tips.
- Total Particulate Exclusion — supports cleaner automated dispensing
- Valve & Sensor Protection — reduces fouling risk in robotic liquid handlers
- Extended Perfusion Stability — helps maintain reliable long-run performance
Inquiry Required: the 0.01 µm ultra-filtered grade is available by request — contact support@diagnocine.com.
Lab-on-Chip Nucleic Acid Analysis
Compatible with microfluidic-based nucleic acid analysis platforms and capillary electrophoresis chips.
Gel Loading, Dilution & Tracking
Increases sample density for well loading and provides visual tracking during electrophoresis.
DNA/RNA Fragment Sizing
Tartrazine and xylene cyanol FF markers bracket a fragment size range of approximately 100 bp to 10 kb for visual monitoring.
Southern & Northern Blotting
Nuclease-tested formulation supports downstream blotting workflows that require intact nucleic acids.
Marker & Ladder Preparation
Convenience of a pre-mixed 6X solution for DNA marker and ladder preparation.
Robotic Liquid Handling & Imaging
Compatible with automated gel-loading and documentation systems used in high-throughput labs.
Detailed product parameters
All values below reflect DCP-YCLB6X as manufactured and quality-controlled by DiagnoCine Precision.
| Parameter | Specification |
|---|---|
| Formulation | 6X Concentrate |
| Appearance | Blue color |
| pH USP <791> | 7.6 |
| Tris-HCl Concentration | 10 mM |
| EDTA Concentration | 60mM |
| Glycerol Concentration | 30% |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase Activity | None detected (18 hours, room temperature, plasmid DNA incubation) |
| RNase Activity | None detected (18 hours, room temperature, ribosomal RNA incubation) |
| Water Quality | Ultrapure Type 1 water, 18.2 MΩ·cm |
| Manufacturing Standard ISO 13485 | ISO 13485-certified and CE-approved facilities |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Size | 5 x 1 mL |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified facilities (DiagnoCine Precision suppliers) |
| Regulatory Alignment | CE-approved |
| Final Packaging & QA Location | Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Every component of DCP-YCLB6X and its stated concentration, presented per DiagnoCine Precision's lot-release basis.
| Component | CAS Number | Concentration |
|---|---|---|
| Glycerol | 56-81-5 | 30% |
| Tartrazine | 1934-21-0 | 0.3% |
| Xylene Cyanol FF | 2650-17-1 | 0.03% |
| Tris-HCl | 1185-53-1 | 10 mM |
| EDTA | 60-00-4 | 60mM |
Manufacturing & compliance
DCP-YCLB6X is manufactured, filtered, and quality-controlled under a documented, traceable process.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (DiagnoCine Precision suppliers).
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
Quadruple-Stage Sterile Filtration
Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in an ISO Class 5 (Class 100) fill environment.
Micro-Batch Precision
Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; specific customization requests and assembly are completed at DiagnoCine Precision in Totowa, New Jersey, USA.
DNase Activity Testing
None detected after incubation of plasmid DNA with this product for 18 hours at room temperature.
RNase Activity Testing
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hours at room temperature.
pH Verification (USP <791>)
Confirmed at pH 7.6.
Documentation / CoA
A Certificate of Analysis is available for this lot upon request.
How DCP-YCLB6X compares
A side-by-side look at DCP-YCLB6X against conventional 0.22 µm-filtered loading buffers.
| Parameter | DCP-YCLB6X (FluxMPS™) | Conventional Loading Buffer (0.22 µm filtered) | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Dual-Color DNA Tracking Dyes | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| DNase-Tested Formulation | check_circle | cancel | cancel |
| RNase-Tested Formulation | check_circle | cancel | cancel |
| Ultrapure Type 1 Water (18.2 MΩ·cm) | check_circle | cancel | cancel |
| ISO 13485-Certified Manufacturing | check_circle | cancel | cancel |
| Microfluidic / Lab-on-Chip Compatibility | check_circle | cancel | cancel |
| Custom Dye / pH / Concentration Formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-YCLB6X Yellow/Xylene Cyanol Loading Buffer [6X].
Supporting literature
Curated peer-reviewed literature relevant to nucleic acid gel electrophoresis, loading buffer chemistry, and microfluidic analysis.
- Lee PY, Costumbrado J, Hsu CY, Kim YH. Agarose gel electrophoresis for the separation of DNA fragments. J Vis Exp. 2012;62:e3923. doi:10.3791/3923
- Green MR, Sambrook J. Separation of DNA fragments by agarose gel electrophoresis. Cold Spring Harb Protoc. 2019;2019(1). doi:10.1101/pdb.top100388
- Voytas D. Agarose gel electrophoresis. Curr Protoc Mol Biol. 2001;51(1):2.5A.1-2.5A.9. doi:10.1002/0471142727.mb0205as51
- Bousse L, Mouradian S, Minalla A, Yee H, Williams K, Dubrow R. Protein sizing on a microchip. Anal Chem. 2001;73(6):1207-1212. doi:10.1021/ac0007349
- Mueller O, Hahnenberger K, Dittmann M, et al. A microfluidic system for high-speed reproducible DNA sizing and quantitation. Electrophoresis. 2000;21(1):128-134. doi:10.1002/(SICI)1522-2683(20000101)21:1<128::AID-ELPS128>3.0.CO;2-F
- Bartram AK, Poon C, Neufeld JD. Nucleic acid contamination of molecular biology reagents. Biotechniques. 2009;46(6):e1-e4. doi:10.2144/000113137
- Hengen PN. Tracking dyes for gel electrophoresis. Trends Biochem Sci. 1994;19(7):304-305. doi:10.1016/0968-0004(94)90079-5
- Studier FW. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973;79(2):237-248. doi:10.1016/0022-2836(73)90003-X
- Kricka LJ, Wilding P. Microchip PCR. Anal Bioanal Chem. 2003;377(5):820-825. doi:10.1007/s00216-003-2144-2
