Blasticidin (10 mg/mL)
Cat No.: DCP-BCSH100MG
Size: 10 mL (10 x 1mL)
Sterile; Ultrapure, Filtered 0.22-micron membrane Once.
Description:
Blasticidin (10 mg/mL) in HEPES buffer is a sterile antibiotic solution used for selection of mammalian cells carrying the bsd or bsr resistance genes. It is typically supplied as a ready-to-use stock in 20 mM HEPES at pH 7.4, which supports convenient dilution into culture medium for routine selection workflows.
Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes and acts as a rapid inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. In cell culture, it is widely used as a selection agent because sensitive cells die quickly, often within about 10–14 days under selection pressure. The HEPES-buffered formulation helps maintain solution stability and makes the stock easy to handle in culture-based applications.
Applications
This product is commonly used for stable selection after transfection, transduction, or genome engineering when the expression cassette includes a blasticidin resistance gene. It is also used to maintain selective pressure in established stable cell lines. Because blasticidin acts quickly, it is useful when investigators want a short selection window and efficient elimination of non-resistant cells.
Quality Control:
- Appearance: Clear, Colorless Liquid.
- pH: 7.4
- Sterility: Filtered 0.1-micron membrane Once.
- DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: -80℃.
- Shelf life: 24 months (unopened)
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
