FSD Fluor™ 594 NHS ester

Product#: KOSC1001

$3,943.36

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FSD Fluor™ 594 NHS ester

Cat. No. List below

Description

FSD Fluor™ 594 NHS ester is a cutting-edge fluorescent dye developed by BioActs, offering superior optical properties compared to spectrally similar dyes. This bright orange, amine-reactive dye is designed for excellent performance in various biochemical and biological analytical applications.

FSD Fluor™ 594 is ideal for single-molecule detection of bioconjugates, fluorescence correlation spectroscopy, and fluorescence polarization measurements. The dye exhibits excellent fluorescence intensity when bound to biomolecules such as antibodies, nucleotides, and proteins. It can be excited using 561, 568, or 578 nm laser lines, displaying exceptional optical properties.

As an NHS ester, FSD Fluor™ 594 readily reacts with amine-modified oligonucleotides, amino groups of proteins (e.g., ε-amino groups of lysine), and amine terminus of nucleotides. This reactivity forms a chemically stable amide bond between the dye and the target biomolecule. The dye can be conjugated to low-abundance biomolecules with high sensitivity and molar ratios, enabling sensitive detection.

FSD Fluor™ 594 NHS ester is optimized for labeling antibodies, peptides, proteins, ligands, and amplification substrates. These labeled molecules are particularly suited for cellular labeling and detection.

FSD Fluor™ 594 NHS ester represents a significant advancement in fluorescent dye technology, offering researchers a powerful tool for various analytical and imaging applications in life sciences.

FSD Fluor™ 594 NHS ester offers several advantages over other spectrally similar dyes like Alexa Fluor 594 and DyLight 594:

Advantages:	FSD Fluor™ 594:	Alexa 594 / DyLight 594:
Fluorescence Intensity	Up to 30% higher	Standard
Quantum Yield	Excellent	Standard
Photostability	Enhanced	Standard
Single-Molecule Detection	Ideal	Standard
Sensitivity for Low-Abundance Targets	High	Standard
Background Reduction	Higher signal-to-noise ratio	Standard
Cost-Effectiveness	Cost-effective	Standard
Wide pH Range	Broad pH range (4-9)	Standard
Versatile Applications	Suitable for various applications	Standard

Specifications

Fluorophore: FSD Fluor™ 594
Reactive group: NHS ester
Excitation/Emission Max.(nm): 593/618
Spectrally similar dyes: Alexa594, DyLight594
Extinction coefficient: ≥ 81,000 cm-1M-1
CF280: 0.56
Appearance: Purple Liquid
Molecular Weight: 1104.60 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm	Spectrally similar dyes
KOSC1002	FSD Fluor™ 488	495	519	Alexa Fluor 488, FAM, Cy2
KOSC1003	FSD Fluor™ 555	554	565	Alexa Fluor 555, DyLight 549, Cy3, ATTO 550
KOSC1001	FSD Fluor™ 594	591	617	Alexa Fluor 594, DyLight 594
KOSC1315	FSD Fluor™ 647	651	667	Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650
KOSC1515	FSD Fluor™ 680	679	696	Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680
KOSC1702	FSD Fluor™ 750	749	774	Alexa Fluor 750, DyLight 755, Cy7, IRDye 750
POSC1803	FSD Fluor™ 800	774	790	Cy7.5, DyLight 800, IRDye 800

Background

FSD Fluor™

FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.

Superior fluorescence intensity than any other spectrally similar dyes
Maintaining excellent fluorescence after conjugated to biomolecules, surpassing that of any other spectrally similar fluorophore
conjugated biomolecules.
Higher quantum yield comparing to traditional dyes
Equipped with variety of reactive group
Covering the full fluorescence spectral range from visible to NIR

Figure 1. Fluorescence intensity comparison of FSD Fluor™ and other dyes

Figure 2. Fluorescence intensity comparison in varying dye/protein ratio

Figure 3. Immunofluorescence comparison of dye-antibody conjugates and photostability test of fluorescent secondary antibodies

Figure 4. Biodistribution images of FSD Fluor™ 750 and FSD Fluor™ 800

Citation & Reference

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