FSD Fluor™ 800 NHS ester

Product#: POSC1803
$3,943.36

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FSD Fluor™ 800 NHS ester

Cat. No. List below

Description

FSD Fluor™ 800 NHS ester is a state-of-the-art, amine-reactive near-infrared (NIR) fluorescent dye developed by BioActs. This innovative dye offers exceptional optical properties, surpassing those of spectrally similar fluorophores, making it an excellent choice for a wide array of biochemical and biological analytical applications.

Key Features
1. Superior optical properties compared to similar fluorophores
2. Excellent fluorescence intensity after binding to biomolecules
3. Compatible with most common fluorescence imaging systems
4. Part of the FSD Fluor™ series, covering a wide spectral range from visible to NIR
5. Designed for complex detection in life science research
6. High quantum yield compared to traditional dyes

Applications
1. In Vitro Imaging: Conjugated primary and secondary antibodies serve as molecular probes
2. In Vivo NIR Imaging: Optimized for labeling antibodies, peptides, proteins, ligands, and amplification substrates
3. Molecular Biology: Amine-modified oligonucleotide labeling
4. Cell Biology: Protein tagging and cellular imaging
5. Medical Imaging: Deep tissue penetration for non-invasive diagnostics

Advantages
1. Enhanced Stability: Improved photostability of dye-protein conjugates
2. Superior Brightness: Maintains excellent fluorescence after conjugation to biomolecules
3. Reduced Background: Lower non-specific binding, resulting in improved signal-to-noise ratios
4. Versatility: Ideal for single-molecule detection, fluorescence correlation spectroscopy, and fluorescence polarization measurements

FSD Fluor™ 800 is compatible with various fluorescence imaging systems, including:
-Confocal microscopes with 750 or 785 nm laser excitation
-Flow cytometers with red laser excitation
-In vivo imaging systems with NIR capabilities

This cutting-edge dye is designed to meet the demanding requirements of complex detection in life science research, offering researchers a powerful tool for their analytical needs. Its superior properties make it an ideal choice for applications ranging from in vitro and in vivo imaging to molecular and cellular biology studies, as well as advanced medical imaging techniques.

 
Specifications
  • Fluorophore: FSD Fluor™ 800
  • Reactive group: NHS ester
  • Excitation/Emission Max.(nm): 774/790
  • Spectrally similar dyes: Cy7.5, DyLight800, IRDye800
  • Extinction coefficient: ≥ 232,000 cm-1M-1
  • CF280: 0.03
  • Appearance: Green Liquid
  • Molecular Weight: 1399.03 g/mol
  • Solubility: DMF, DMSO
  • Storage conditions: -20 ℃, protect from light
Quick link (Cat.#) FSD series EXmax (nm) EMmax (nm Spectrally similar dyes
KOSC1002 FSD Fluor™ 488 495 519 Alexa Fluor 488, FAM, Cy2
KOSC1003 FSD Fluor™ 555 554 565 Alexa Fluor 555, DyLight 549, Cy3, ATTO 550
KOSC1001 FSD Fluor™ 594 591 617 Alexa Fluor 594, DyLight 594
KOSC1315 FSD Fluor™ 647 651 667 Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650
KOSC1515 FSD Fluor™ 680 679 696 Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680
KOSC1702 FSD Fluor™ 750 749 774 Alexa Fluor 750, DyLight 755, Cy7, IRDye 750
POSC1803 FSD Fluor™ 800 774 790 Cy7.5, DyLight 800, IRDye 800
 
Background
FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.

Key Benefits: 
  • Superior brightness: up to 30% brighter than leading competitors, enabling detection fo low-abundance targets. 
  • Excellent Stability: Maintains high fluorescence intensity after bioconjugation
  • Versatile Applications: Ideal for antibody labeling, nucleic acid detection, and protein studies
  • Optimized for Single-Molecule Detection: Perfect for fluorescence correlation spectroscopy and polarization measurements
  • Higher quantum yield comparing to traditional dyes
  • Equipped with variety of reactive group
  • Covering the full fluorescence spectral range from visible to NIR
  • Enhanced Sensitivity: Detect targets with greater accuracy, even at low concentrations
  • Reduced Background: Higher signal-to-noise ratio for clearer images and more reliable data
  • Cost-Effective: Achieve superior results with less dye, maximizing your research budget

 

Figure 1.   Fluorescence intensity comparison of FSD Fluor™ and other dyes

 

Figure 2.   Fluorescence intensity comparison in varying dye/protein ratio

 

Figure 3.   Immunofluorescence comparison of dye-antibody conjugates and photostability test of fluorescent secondary antibodies

 

 Figure 4.   Biodistribution images of FSD Fluor™ 750 and FSD Fluor™ 800


Citation & Reference

1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.
 
 
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