High Activity Adipose Tissue Enzymatic Digestion Kit (Mouse and Rat), 50T
High Activity Adipose Tissue Enzymatic Digestion Kit (Mouse and Rat), 50T
Cat No.: RWD-DHAE-5010Number of reactions: 50T
Description
The High Activity Adipose Tissue Enzymatic Digestion Kit (Mouse and Rat) enables gentle, rapid, and efficient preparation of high-viability single-cell suspensions from mouse and rat adipose tissues. The optimized protocol maximizes recovery and viability of single cells while preserving key surface antigen epitopes. The resulting suspensions consist primarily of non-mature adipose cells, making them ideal for SVF (stromal vascular fraction) cell culture, cell sorting, and downstream applications such as single-cell sequencing.
Mouse white adipose
The test showed a cell viability rate of 91.80%, with a total cell yield up to 0.57 × 10^6 cells per 100 mg tissue. The average cell diameter in the single-cell suspension was 12.41 μm (viable cells: 12.55 μm; dead cells: 11.07 μm).
Primary Cell Cultures
Primary culture results for single-cell suspensions from both white and brown adipose tissues showed good cell status. Under the microscope, cells were observed to adhere and grow well. High-quality single-cell suspensions support successful downstream primary culture experiments.
Scope of Application:
| Adipose type | Type of cells |
| White adipose | Immature adipocytes (Stromal Vascular Function cells) |
| Beige adipose | |
| Brown adipose |
FAQs
Q: What are the storage requirements for the kit?
A: Storage temperature depends on the types of enzymes used. Store each kit at 4°C or –20°C as specified in the instructions.
Q: What precautions are needed during tissue pre-processing?
A: Carefully remove all blood vessels and residual clots from samples, and rinse tissues thoroughly to ensure complete cleanliness before digestion.
Q: What is the main target cell population extracted by this kit?
A: The kit focuses on isolating non-mature adipose cells—i.e., SVF cells—including fibroblasts, endothelial cells, macrophages, stromal cells, immune cells, and preadipocytes.
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