Columbia Agar Base Plates/W Penicillin G
Catalog No: DCP-CABPPG
pH: 7.3 ± 0.2
Uses: Standard method base for enumeration and detection of bacteria in food and environmental samples, and clinical speciments
Size: 100mm
Concentration: 10 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: light amber, clear to slightly opalescent firm gel. Adding 5% sheep (or horse/rabbit) blood, the medium becomes opaque red
Sterile
Shelf life: 2 Weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Columbia Agar base is a highly nutritious, general-purpose medium developed for the isolation and cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and non-clinical materials. It is frequently used as a base for selective media formulations, where different combinations of antimicrobial agents can be added to enhance selectivity for specific organisms.
The rich nutrient composition—derived from a blend of peptones, heart and meat extracts, and yeast extract—provides essential amino acids, vitamins (including those of the B complex), and other growth factors necessary for luxuriant bacterial growth. Sodium chloride maintains osmotic balance, while maize starch serves as an energy source and helps neutralize toxic metabolites.
When supplemented with Penicillin G, a β-lactam antibiotic, Columbia Agar base becomes more selective by inhibiting many penicillin-sensitive Gram-positive bacteria and some susceptible Gram-negative species, depending on their intrinsic and acquired resistance.. This allows for improved isolation and detection of penicillin-resistant bacteria and certain gram-negative species in samples with mixed microbial populations. The medium can be applied to samples such as food, dairy, water, and pharmaceuticals, especially in contexts where penicillin-susceptible background flora need to be suppressed
Columbia Agar base with Penicillin G is especially useful in applications where suppression of background flora is needed to recover target organisms, such as in the detection of resistant strains or the isolation of specific pathogens from complex specimens.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
