FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMA-R1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose — ATCC Modification — Phenol Red-Free formulation, engineered for research protocols where ATCC-validated formulation control and microfluidic-grade purity are both required. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 4.5 g/L Glucose, [+] L-Glutamine, [+] 1 mM Sodium Pyruvate, [+] 1.5 g/L Sodium Bicarbonate, [+] Calcium, [+] Magnesium | [-] Phenol Red.
- ATCC Modification: 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate, matching ATCC’s validated formulation for its authenticated reference cell line panel
- Phenol red-free formulation removes the dye’s own visible-light absorbance, supporting fluorescence-based assays and ER-positive cell line work
- Compatible with ATCC authentication protocols for HeLa, MCF-7, A549, HEK-293, and Vero reference lines
- Quadruple-stage filtration (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm final polish) reaching a 0.04 µm final pore size for microfluidic channel compatibility
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- 5% CO2 incubation recommended, consistent with the 1.5 g/L sodium bicarbonate buffering system
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Custom formulation modifications (pH, glucose, salts) available on request
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose (4.5 g/L), [+] Sodium Pyruvate (1 mM) | [-] Phenol Red
- AppearanceColorless (phenol red-free), clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- CO2 Requirement5% CO2 required (sodium bicarbonate-buffered)
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
The ATCC-specified formulation for authenticated cell line culture
ATCC (American Type Culture Collection) maintains specific media formulations validated against its authenticated reference cell lines. The ATCC Modification of DMEM uses precisely 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate — concentrations validated for ATCC-maintained cell lines including HeLa, MCF-7, A549, HEK-293, and Vero. Removing phenol red eliminates the dye’s own absorbance for fluorescence assays and hormone-sensitive cell lines. FluxMPS™ adds quadruple-stage 0.04 µm filtration to enable use on microfluidic and organ-on-a-chip platforms.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in chip geometries, including sub-100 µm channels.
ATCC-validated formulation
1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate match ATCC’s specification for its authenticated reference cell line panel.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) supports trace-metal and organic-carbon (TOC) control for sensitive analytical and imaging assays.
Low background for imaging
Phenol red-free formulation removes the pH indicator’s own visible-light absorbance interference. Combined with 0.04 µm final filtration, particulate baseline is minimized for confocal imaging and biosensor applications.
High-glucose energy support
4.5 g/L glucose plus 1 mM sodium pyruvate sustain the energy demands of glycolytically active and OXPHOS-dependent reference cell lines.
Customization on demand
pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma-retentive purity beyond conventional 0.22 µm filtered media.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2 effluent, but redundancy for Stage 4.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish in ISO Class 5 (Class 100) laminar-flow conditions.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMA-R1X
Specialized applications requiring ATCC Modification — Phenol Red-Free
FluxMPS™ DCP-DMEMA-R1X is designed for research protocols where ATCC Modification — Phenol Red-Free conditions are essential, combined with Microfluidics Suitable filtration purity for organ-on-a-chip and microfluidic platform compatibility.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available for automated bioreactor perfusion and robotic liquid handlers — see the Grade note in the filtration section above.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion circuits
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Organ-on-a-Chip & MPS
Ultra-clean 0.04 µm filtration prevents microchannel clogging. ATCC Modification — Phenol Red-Free formulation delivered particle-controlled for specialized OoC protocols.
HeLa Culture
Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for HeLa reference-line culture.
MCF-7 & Cancer Lines
Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for MCF-7 and related cancer cell line platforms.
HEK-293 Expression
Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for HEK-293 expression platforms.
Vero Cell Culture
Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for Vero cell culture platforms.
ATCC-Protocol Compatible
Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for ATCC-protocol compatible research platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose (4.5 g/L), [+] Sodium Pyruvate (1 mM) | [-] Phenol Red |
| Appearance | Colorless (phenol red-free), clear solution |
| Glucose | 4500 mg/L (4.5 g/L, per ATCC specification) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | 110 mg/L (1 mM) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 32 ingredients across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 browsable tabs |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (see § Manufacturing & Compliance for batch-release testing) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2-8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | 5% CO2 required (derived from 1.5 g/L sodium bicarbonate) |
| Pack sizes available | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose — ATCC Modification — Phenol Red-Free: 32 ingredients verified per lot with CAS numbers for full raw-material traceability. ATCC Modification formulation: 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate. Phenol red is absent. These concentrations are validated for ATCC-maintained cell lines — do not substitute standard DMEM for ATCC-protocol experiments.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 1500.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feed water — supports trace-metal and organic-carbon (TOC) control for sensitive assay outcomes.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMA-R1X compares
FluxMPS™ DCP-DMEMA-R1X vs. conventional 0.22 µm-filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMA-R1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade (no MPS designation) | Standard grade (no MPS designation) |
| DMEM ATCC Modification — 1.5 g/L bicarbonate validated for ATCC reference cell lines, phenol red-free for imaging | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle Yes (0.1 µm mycoplasma-retentive) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance (Method 1) | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMA-R1X — DMEM High Glucose ATCC Modification — Phenol Red-Free.
Supporting literature
Key publications supporting the research applications of FluxMPS™ DCP-DMEMA-R1X.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
- Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216-4220. doi:10.1073/pnas.77.7.4216
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
- Lincoln B, et al. ATCC animal cell culture guide: reference cell line authentication and quality control. ATCC Technical Bulletin. 2019. atcc.org
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j
