High Activity Adult Brain Enzymatic Digestion Kit(Mouse and Rat), 50T
High Activity Adult Brain Enzymatic Digestion Kit(Mouse and Rat), 50T
Cat No.: RWD-DHABE-5003Number of reactions: 50T
The High Activity Adult Brain Enzymatic Digestion Kit (Mouse and Rat) utilizes a combined approach of mechanical shearing and enzymatic extracellular matrix digestion to produce highly viable single-cell suspensions from adult mouse and rat brain, hippocampus, cortex, and spinal cord tissues (recommended for animals older than 7 days, typically P9–12 weeks). The optimized formula enables gentle, fast, and efficient dissociation, maximizing cell recovery and viability while preserving critical surface antigen epitopes.
Mouse brain
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Using standardized protocols, viability measured at 93.86% and total cell yield reached 1.03 × 10^6 cells per 100 mg adult mouse brain tissue.
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Cell diameter analysis: total cells 12.58μm, viable cells 12.63μm, non-viable cells 11.80μm.
Flow cytometry analysis of mouse brain
The kit digests tissue to isolate neurons, endothelial cells, microglia, oligodendrocytes, astrocytes.
Primary culture
The primary cells are in good condition and can be seen growing adherently.
FAQs
Q: What are the storage conditions for enzymatic digestion kits?
A: Storage requirements differ by enzyme type. Follow the instructions in each kit’s manual: store enzymes at either 4°C or –20°C according to specific guidelines.
Q: What are the tissue pre-processing precautions?
A: During sample collection, thoroughly remove all blood vessels and residual clots; rinse the tissue multiple times to ensure it is clean before proceeding with digestion.
Q: What animals and age range is the Adult Brain Kit suitable for?
A: For mice and rats older than seven days (P > 7); optimized for adult neural tissues, especially P9 to 12 weeks.
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