High Activity Tumor Tissue Enzymatic Digestion Kit (Human), 25T

Cat No.: RWD-DHTEH-2505
Number of reactions: 25T
   

Description

The High Activity Tumor Tissue Enzymatic Digestion Kit (Human) enables gentle, rapid, and efficient preparation of viable single-cell suspensions from primary human tumor tissue or xenografts. This optimized protocol maximizes single-cell yield and viability, preserving critical surface antigen epitopes needed for downstream applications. Resulting suspensions are ideal for cell sorting, tumor cell or TIL (tumor-infiltrating lymphocyte) culture, and single-cell sequencing.
 

---

Human breast cancer

Results show total cell viability generally > 90%.

Flow cytometry analysis of mouse tumor

Using RWD human tumor tissue gentle digestion kit, single-cell suspension preparation instrument, and tissue processing tubes, combined with standardized procedures, single-cell suspensions of various tumor tissues were analyzed by flow cytometry for cell subpopulation surface marker retention.

To improve data reliability, tumor tissues such as colorectal cancer, melanoma, lung cancer, and breast cancer were processed. Flow data demonstrates that high-quality single-cell suspensions retain a broad array of markers (CD24, CD44, CD90, CD133), enabling sorting and identification of tumor cell subtypes and ensuring good marker preservation.

Primary Cell Cultures

Using RWD human tumor tissue gentle digestion kit and standardized process, viable single-cell suspensions support high-quality primary cell cultures.

Cells adhere and grow well, form epithelial-like and fibroblast-like colonies, and proliferate rapidly. The described single-cell suspensions support high-quality and efficient downstream primary cell experiments.

---

Scope of Application:

Tumor Types	Examples
Soft tissue	Melanoma, ovarian cancer, colon cancer, nasopharyngeal carcinoma, renal clear cell adenocarcinoma, etc
Medium-hard tissue	Lung cancer, prostate cancer, etc
Hard tissue	Breast cancer, pancreatic cancer, liver cancer, head and neck squamous cell carcinoma (HNSCC), etc

FAQs

Q: What are the storage requirements for this kit?
A: The enzyme composition differs between kits; always store components as instructed, either at 4°C or –20°C as specified in the manual.

Q: What are the tissue pre-processing precautions?
A: During sampling, carefully remove blood vessels and residual clots, and rinse tissue thoroughly to ensure complete cleanliness before enzymatic digestion.

Q: What types of human tumor tissues can be processed?
A: The kit is suitable for tumor samples freshly obtained from patients, for patient-derived xenograft (PDX) samples grown in nude mice, and for human tumor cell lines cultured in nude mice.

Related Products