High Activity Tumor Tissue Enzymatic Digestion Kit (Mouse), 50T

Cat No.: RWD-DHTE-5001
Number of reactions: 50T

   

Description

The High Activity Tumor Tissue Enzymatic Digestion Kit (Mouse) enables gentle, rapid, and high-efficiency preparation of single-cell suspensions from transplanted mouse tumor tissues. The formulation is optimized to maximize total cell yield and viability while effectively preserving critical cell surface antigen epitopes. Single-cell suspensions obtained using this kit are highly suitable for downstream applications, including primary culture of tumor cells or tumor-infiltrating lymphocytes (TILs), cell sorting, and single-cell sequencing.

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Mouse colon cancer


 

 

Using this kit with a single-cell suspension dissociator and processing tubes under standardized protocols, viability reached 98.24% and total cell count up to 30 × 10^6 cells per 100 mg tissue.

Mean diameters: total cells 14.14μm, live cells 14.16μm, dead cells 12.55μm.

Mouse breast cancer

Standardized processing yielded viability of 97.59% and total cell yield up to 10 × 10^6 cells per 100 mg tissue.

Mean diameters: total cells 14.60μm, live cells 14.72μm, dead cells 12.49μm.
 

Flow cytometry analysis of immune cells in mouse tumor tissues

Immunophenotyping using surface markers CD45, CD3, CD4, CD8, CD44 enables identification of major immune cell types and tumor subpopulations, confirming high-quality enzymatic processing and effective preservation of antigen markers.

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Storage

Different kits may include different enzyme types. Always refer to the kit instructions:
Store enzymes as directed at 4°C or –20°C.

 

Tissue Sample Pre-Processing Precautions

• Carefully remove blood vessels and any residual blood clots during tissue collection.
• Rinse tissue repeatedly with buffer until thoroughly cleaned of contaminants and blood residues.

Requirements for Preparing Single-Cell Suspensions from Mouse Tumor Tissue

• Confirm the tissue type before processing.
• For human-derived tumors engrafted in mice (PDX), use the Human Tumor Tissue Digestion Kit.
• Always consult the appropriate enzymatic digestion kit manual for any tissue-specific processing steps or recommendations.
• Follow protocol adjustments found in the dedicated kit instructions for optimal yield and cell viability.

Scope of Application:
 

Tumor Types	Examples
Soft tissue	Melanoma, colon cancer
Medium-hard tissue	Lung cancer
Hard tissue	Breast cancer,liver cancer

  

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