High Activity Neonatal Brain Enzymatic Digestion Kit(Mouse and Rat), 50T

Cat No.: RWD-DHNBE-5002
Number of reactions: 50T

   

Description

The High Activity Neonatal Brain Enzymatic Digestion Kit (Mouse and Rat) enables gentle, rapid, and efficient dissociation of neonatal mouse and rat brain tissue (including embryonic and postnatal brains, P ≤ 7) into high-viability single-cell suspensions. This optimized protocol maximizes cell yield and viability while preserving critical cell surface antigen epitopes. The resulting single-cell suspension is suitable for downstream applications such as primary culture, cell sorting, and single-cell sequencing.
 

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  Mouse neonatal brain

• Using this kit with standardized protocol and single-cell suspension system, the cell viability reached 96.78%.

• Total cell yield was up to 4.77 x 10^6 cells per 100 mg tissue.

• Mean cell diameters: total cells 9.55μm, viable cells 9.62μm, nonviable cells 8.47μm.

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FAQs

Q: What are the storage conditions for brain tissue enzymatic digestion kits?
A: Enzyme composition varies by kit. Always store all kit components according to the product manual. Most enzymes require storage at either 4°C or -20°C, as indicated in the instructions. Do not freeze debris removal reagents unless otherwise specified.

Q: What tissue pre-processing steps are required?
A: Remove all blood vessels and residual clots carefully while collecting the tissue. Rinse brain tissue thoroughly with buffer to remove contaminants and blood before enzymatic digestion.

Q: What age range is the Neonatal Brain Kit suitable for?
A: This kit is suitable for neonatal mice or rats up to seven days old (P ≤ 7).

 

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