What makes the filtration process superior to standard 0.22 micron filtered media?

Standard 0.22 micron filtration does not target mycoplasma-sized particulates (0.2-0.3 micron). FluxMPS uses four sequential stages reaching 0.04 micron, delivering approximately 5x lower particulate counts per USP less than 788 Method 1 (light obscuration).

Does this medium require a CO2 incubator?

This formulation is HEPES-buffered (15 mM) with a reduced sodium bicarbonate level (1200 mg/L), giving reduced CO2 dependency. Standard 5 percent CO2 incubation remains fully compatible; validate against your specific culture vessel and sealing conditions.

FluxMPS Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid

Q: Can DCP-DM12-QT1X be supplemented with serum, growth factors, or other components?

Yes. This medium can be supplemented with FBS (5-20 percent), L-Glutamine, growth factors, antibiotics, or other additives per standard practice. Add supplements immediately before use. Serum and other protein-containing supplements should be pre-filtered through a 0.2 micron low-protein-binding PES or PVDF membrane; do not use a 0.04 micron membrane for supplement filtration.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP less than 791), osmolality (USP less than 785), endotoxin (USP less than 85 BET), sterility (USP less than 71), particulate matter (USP less than 788 Method 1), raw material traceability, manufacturing date, lot number, expiry, and authorized release signatures. Request via support@diagnocine.com.

ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid

Sodium Bicarbonate Phenol Red HEPES Calcium Magnesium Glucose Sodium Pyruvate L-Glutamine

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

Quadruple-stage nano-filtration validated to 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2), delivering approximately 5× lower particulate counts than 0.22 µm-filtered media
Endotoxin release specification < 0.05 EU/mL, verified per manufacturing batch by LAL assay (USP <85> BET)
DMEM/F-12 (1:1) base with 15 mM HEPES buffering and 1200 mg/L sodium bicarbonate for reduced CO₂ dependency
Formulated without L-Glutamine for user-controlled nitrogen source supplementation
High glucose (3151 mg/L) and sodium pyruvate (110 mg/L) support standard energy metabolism protocols
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under ISO Class 5 aseptic fill and finish
Manufactured under an ISO 13485:2016 quality management system with a per-batch Certificate of Analysis
Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request

DCP-DM12-QT1X |Size: 500 mL and 1000 mL |Cell Culture Media

UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid

Glucose3151 mg/L (3.151 g/L)
L-GlutamineNot present — supplement as needed
Sodium Pyruvate110 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)Determined per lot; see Certificate of Analysis
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened
ShippingCold pack (2–8°C)

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and batch-to-batch variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is built around a validated filtration architecture designed to reduce these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filter targets sub-mycoplasma-size particulates; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

Total metabolic control

L-Glutamine is excluded so users can define nitrogen source timing and concentration for their own metabolic flux and Warburg-effect protocols.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) per ASTM D1193 / ISO 3696, supporting low trace-metal and organic-carbon (TOC) background in the feed water.

visibility

Low background for imaging

Reduced particulate load supports cleaner optical fields for confocal microscopy, live-cell biosensors, and TEER measurements by minimizing scatter from subvisible particles.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid is processed through a four-stage serial filtration sequence — two dedicated prefilter + final-filter pairs — reaching a 0.04 µm final pore size.

1

0.1 µm Prefiltration I

Removes large particulates and aggregates; protects the first 0.04 µm final filter cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill & finish in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration.

5×

0.04

µm final filter pore size — sub-mycoplasma polishing

Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled via the 0.1 µm mycoplasma-retentive filtration stage (not tested per lot).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine 1X Liquid (DCP-DM12-QT1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II for organ-on-a-chip and microfluidic applications by Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DM12-QT1X

Applications

Designed for next-generation cell culture platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid is suited to organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this base formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion — this is a distinct, separately named tier from the Microfluidics Suitable product on this page (see the Grade note above).

Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade version.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports microchannel flow integrity in chip-based culture systems.

OoCToCBoCLoCMPS

Cancer Biology

Warburg Effect & Metabolic Research

Defined glucose and pyruvate content with excluded L-Glutamine enables precise nitrogen-source control for metabolic flux analysis.

MCF-7MDA-MB-231HeLaHT-1080

Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where particulate and endotoxin variability drive off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER-based assays.

HUVECsBHK-21Primary fibroblasts

Metabolomics

Metabolic Flux Analysis

Chemically defined base with excluded L-Glutamine supports isotope tracing studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports confocal imaging and biosensor integration by reducing optical scatter and interference.

ConfocalBiosensorsTEER

Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid undergoes the quality-release battery below before shipment.

Physical & Chemical Parameters

Parameter	Specification
Formulation	Contains Sodium Bicarbonate, Phenol Red, HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate; without L-Glutamine
Appearance	Red-colored, clear solution
pH USP <791>	7.4
Osmolality USP <785>	Determined per lot; see Certificate of Analysis
Glucose	3151 mg/L (3.151 g/L)
L-Glutamine	Not present — supplement as needed
Sodium Pyruvate	110 mg/L
Phenol Red	8.630 mg/L (present)
HEPES	3574.500 mg/L (15 mM)
Sodium Bicarbonate	1200.000 mg/L (~14.3 mM)

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth after 14 days
Mycoplasma	Controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	Compliant
Particulate ≥25 µm USP <788> Method 1	Compliant
Water Purity	Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, protected from light
Freeze-thaw	Not recommended
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	HEPES-buffered; reduced CO₂ dependency (~3% CO₂ equivalent from 1200 mg/L sodium bicarbonate); standard 5% CO₂ incubation also compatible

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Cell culture / reagent grade
Traceability	Full lot documentation, CoA available
Manufacturing QMS	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch precision manufacturing
Intended use	For Research Use Only (RUO)
Pack sizes available	500 mL, 1000 mL

Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Total: 52 components across 4 categories. Custom compositions available on request.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	154.500
Copper sulfate pentahydrate	7758-99-8	0.0013
Disodium hydrogen phosphate	7558-79-4	71.020
Ferric nitrate nonahydrate	7782-61-8	0.050
Ferrous sulfate heptahydrate	7782-63-0	0.417
Magnesium chloride hexahydrate	7791-18-6	61.200
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	311.800
Sodium bicarbonate	144-55-8	1200.000
Sodium chloride	7647-14-5	6996.000
Sodium dihydrogen phosphate monohydrate	10049-21-5	54.300
Zinc sulfate heptahydrate	7446-20-0	0.432

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	18.750
L-Alanine	56-41-7	4.450
L-Arginine hydrochloride	1119-34-2	147.500
L-Asparagine monohydrate	5794-13-8	7.500
L-Aspartic acid	56-84-8	6.650
L-Cysteine dihydrochloride	7048-04-6	31.290
L-Cystine hydrochloride monohydrate		17.560
L-Glutamic acid	56-86-0	7.350
L-Histidine hydrochloride monohydrate	5934-29-2	31.480
L-Isoleucine	73-32-5	54.470
L-Leucine	61-90-5	59.050
L-Lysine hydrochloride	657-27-2	91.250
L-Methionine	63-68-3	17.240
L-Phenylalanine	63-91-2	35.480
L-Proline	147-85-3	17.250
L-Serine	56-45-1	26.250
L-Threonine	72-19-5	53.450
L-Tryptophan	73-22-3	9.020
L-Tyrosine disodium salt	69847-45-6	48.100
L-Valine	72-18-4	52.850

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	8.980
D-Biotin	58-85-5	0.0035
D-Ca-Pantothenate	137-08-6	2.240
Folic acid	59-30-3	2.660
Nicotinamide	98-92-0	2.020
Pyridoxal hydrochloride	65-22-5	2.000
Pyridoxine hydrochloride	58-56-0	0.031
Riboflavin	83-88-5	0.219
Thiamine hydrochloride	67-03-8	2.170
Vitamin B12	68-19-9	0.680
OTHERS
myo-Inositol	87-89-8	12.600
D-Glucose	50-99-7	3151.000
DL-Thioctic acid	1077-28-7	0.105
HEPES	7365-45-9	3574.500
Hypoxanthine sodium salt		2.400
Linoleic acid	60-33-3	0.042
Phenol red sodium salt	34487-61-1	8.630
Putrescine hydrochloride	333-93-7	0.081
Sodium pyruvate	113-24-6	110.000
Thymidine	50-89-5	0.365

Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.

Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay on every manufacturing batch. Release specification: < 0.05 EU/mL. Assay sensitivity: 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result reported per lot on the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request via support@diagnocine.com with your lot number.

Product Comparison

How DCP-DM12-QT1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter	DCP-DM12-QT1X (FluxMPS™)	Conventional DMEM/F-12 (0.22 µm)	Standard DMEM/F-12 alternative
Grade	Microfluidics Suitable	Not tiered (0.22 µm)	Not tiered (0.22 µm)
Formulation trait	w/o L-Glutamine, 15 mM HEPES-supplemented	Standard, with L-Glutamine	Standard, with L-Glutamine
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 stages	1 stage	1–2 stages
Mycoplasma-retentive filtration	check_circle	cancel	cancel
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL		Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance	check_circle USP <788> Method 1	cancel	cancel
Water quality	Ultrapure Type 1 (18.2 MΩ)	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	Variable	Variable
Microfluidic channel compatibility	check_circle Suitable	cancel Risk of clogging	cancel Risk of clogging
Custom formulation	check_circle On request	cancel	Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid.

Is DCP-DM12-QT1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-DM12-QT1X is a Microfluidics Suitable formulation processed through Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering low particulate counts that help prevent microchannel clogging in OoC and MPS devices.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Standard 0.22 µm filtration does not target mycoplasma-sized particulates (0.2–0.3 µm). FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1 (light obscuration).

Why is L-Glutamine excluded, and how do I supplement for my cell type?expand_more

L-Glutamine degrades in liquid storage, so it is omitted and added fresh at time of use, typically at 2–4 mM L-Glutamine or a stabilized dipeptide alternative such as GlutaMAX, per your cell line’s requirements. Contact support@diagnocine.com for guidance.

Does this medium require a CO₂ incubator?expand_more

This formulation is HEPES-buffered (15 mM) with a reduced sodium bicarbonate level (1200 mg/L), giving reduced CO₂ dependency. Standard 5% CO₂ incubation remains fully compatible; validate against your specific culture vessel and sealing conditions.

Can DCP-DM12-QT1X be supplemented with serum, growth factors, or other components?expand_more

Yes. This medium can be supplemented with FBS (5–20%), L-Glutamine, growth factors, antibiotics, or other additives per standard practice. Add supplements immediately before use. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for supplement filtration, as it will strip serum proteins and lipoproteins and clog rapidly.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are on the Certificate of Analysis, available from support@diagnocine.com.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, lot number, expiry, and authorized release signatures. Request via support@diagnocine.com.

Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered media and microfluidic cell culture applications.

Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o L-Glutamine: 1X Liquid