Hank's Balanced Salt Solution w/o Calcium, Magnesium and Phenol Red: 1X
Cat No.: HBSS-CMR1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[-] Calcium Chloride, [-] Magnesium Sulfate, [-] Sodium Bicarbonate, [-] Magnesium Chloride, [-] Phenol Red
Description:
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). Hank's Balanced Salt Solution (HBSS) 1X Without Calcium Chloride, Magnesium Sulfate, and Phenol Red is a specialized cell culture reagent designed for applications requiring controlled ionic balance in low-CO₂ or CO₂-free environments. This formulation excludes components that could precipitate or interfere with specific experimental protocols while maintaining cellular osmotic balance. This formulation omits calcium and magnesium ions to prevent cell aggregation, making it ideal for applications requiring cell dissociation, clumping reduction, or low-density cultures. The exclusion of phenol red eliminates potential interference with light-sensitive assays or cloning efficiency.
Applications:
- Cell washing prior to dissociation.
- Transporting cell/tissue samples.
- Cell counting and dilution procedures.
- Suspension cultures requiring reduced adhesion.
- Experiments sensitive to phenol red.
Advantages:
- Calcium and Magnesium exclusion: Omits calcium and magnesium ions to prevent cell clumping, ideal for enzymatic dissociation protocols or suspension cultures where aggregation must be minimized.
- Assay Compatibility: Excludes phenol red, eliminating optical interference in fluorescence microscopy, flow cytometry, or cloning efficiency assays.
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Disodium Hydrogen Phosphate |
48.00 |
| Potassium Chloride |
400.000 |
| Potassium Dihydrogen Phosphate Anhydrous |
60.000 |
| Sodium Chloride |
8000.000 |
| Sodium Bicarbonate |
350.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
Quality Control:
- Appearance: Clear solution.
- pH: 7.4
- Osmolality in mOsm/Kg H2O: 260.00 - 300.00
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
