Prestained Protein Marker Ⅳ (8-200 kDa)
Cat. No.: SB-G2083-250UL
Size: 250UL
Description
Prestained Protein Marker Ⅳ consists of 11 highly purified and pre-stained recombinant proteins. The indicated molecular weight range in Tris-Glycine Gel was 10-200 kDa (~10, ~15, ~20, ~30, ~40, ~55, ~65, ~90, ~110, ~140, ~200 kDa). 90 and 30 kDa are orang e-red bands. 10 kDa is rose-red band; All others are blue bands. Therefore, it is convenient to observe the Electrophoretic state of protein dynamically or judge the effect of protein transmembrane. It is suitable for SDS-PAGE and Western blot.
Storage and transportation
Ship with wet ice; Store at -20℃, valid for 12 months.
Product Components
| Component Number |
Component |
G2083-250UL |
| G2083-1 |
Prestained Protein Marker Ⅳ |
250 μL |
| Product Manual |
1 copy |
Assay Protocol / Procedures
- 1. This product is ready-to-use, without adding reducing agent or heating. Remove Prestained Protein Marker stored at -20 ℃ before thawing at room temperature and mix gently and thoroughly
- 2. Take 5-10 μL (G2083) and the experimental samples for protein electrophoresis; It is suggested that qualified laboratories can determine the appropriate sample amount by pre-experiment according to their own experimental conditions and experimental habits when using this product for the first time, which can not only save costs, but also obtain better experimental pictures.
- 3. Store at -20 ° C immediately after using Prestained Protein Marker (It is recommended that 5-10 μL be stored separately).
note
- 1. This pre-dyed protein molecular weight standard cannot be heated or boiled at 100 ℃ , which will lead to degradation or decolorization of protein bands.
- 2. It is necessary to prolong the transmembrane time or increase the transmembrane voltage for Western blot of large molecular weight proteins.
- 3. The molecular weight of reference prestained proteins in the figure is calibrated according to the molecular weight of non-prestained proteins.
- 4. For your safety and health, please wear a lab coat and disposable gloves when operating.